scholarly journals Study the Thorax Ossification Centers in Partridge Embryos by Alizarin Red and Alcian Blue Double Staining Techniques

2021 ◽  
Vol 52 (1) ◽  
pp. 39-46
Author(s):  
Masoud Sahimirad ◽  
Hassan Gilianpour ◽  
Mohammadreza Paryani ◽  
Bijan Radmehr ◽  
Abbas Veshkini
Keyword(s):  
Alcian Blue ◽  
Double Staining ◽  
Alizarin Red ◽  
Staining Techniques ◽  
Ossification Centers

Double staining technique for rat foetus skeletons in teratological studies

1979 ◽  
Vol 13 (4) ◽  
pp. 309-310 ◽  
Author(s):  
J. Whitaker ◽  
Kathleen M. Dix
Keyword(s):  
Alcian Blue ◽  
Staining Technique ◽  
Alizarin Red S ◽  
Double Staining ◽  
Alizarin Red

Cartilage and bone were differentiated using alcian blue and alizarin red S respectively. Anomalies of both cartilaginous and bony parts of the skeleton could be examined.

scholarly journals Comparing two methods of plastination and glycerin preservation to study skeletal system after Alizarin red-Alcian blue double staining

2013 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Bahman Rashidi ◽  
MohsenM Setayesh ◽  
EbrahimEsfandiari ◽  
AbbasA Rabiei ◽  
MahsaS Hanaei
Keyword(s):  
Alcian Blue ◽  
Double Staining ◽  
Skeletal System ◽  
Alizarin Red

scholarly journals Técnica de diafanización para describir el desarrollo embrionario del sistema óseo. Revisión de la literatura

2017 ◽  
Vol 57 (4) ◽  
pp. 488-501
Author(s):  
David Sandoval ◽  
Jelua Tellez ◽  
Guillermo Rivera ◽  
Sandra Moreno ◽  
Freddy Moreno
Keyword(s):  
Alcian Blue ◽  
Double Staining ◽  
Medical Subject Headings ◽  
Alizarin Red ◽  
Subject Headings

Introducción: La técnica anatómica de diafanización consiste en transparentar los tejidosblandos de organismos vertebrados (aclaramiento), para teñir los tejidos mineralizadosy poder visualizar los componentes óseos y cartilaginosos (tinción). Objetivo: Revisarlos reportes disponibles en la literatura especializada que han descrito el desarrolloembrionario del sistema óseo de mamíferos a través de técnicas de diafanización ytinción simple o tinción doble. Materiales y métodos: Se revisó la literatura sobreel tema en PubMed, Google Académico y SciELO, basándose en la metodologíaPRISMA, a través de la asociación del término double staining con los descriptores ensalud del Medical Subject Headings (MeSH) alizarin red, alcian blue, bone y cartilage,y la combinación con los operadores boleanos + y &. Resultados: En esta revisión dela literatura se incluyeron 22 artículos que describieron la técnica de diafanizacióny tinción simple o tinción doble empleada en la observación, registro y análisis del desarrollo embrionario del sistema óseo de mamíferos.Conclusión: La diafanización y tinciónsimple o tinción doble es una técnica anatómicade estudio —versátil y de bajo costo— del desarrolloembrionario del sistema óseo, la cualpuede emplearse en estudios toxicológicos, paradescartar la posibilidad de anomalías de desarrollodurante la formación del cartílago (condrogénesis)y del hueso (osteogénesis), asociadas a laexposición de un posible agente teratógeno.

scholarly journals Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton

2016 ◽  
Vol 5 (1) ◽  
pp. 19
Author(s):  
Muhammad Ja’far Luthfi
Keyword(s):  
Alcian Blue ◽  
Anatomical Study ◽  
Alizarin Red S ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Double Staining ◽  
Alizarin Red ◽  
Important Method ◽  
Clearing Method ◽  
And Cartilage

<p>Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.</p>

Mineralization in the syrinx and caudal tracheal rings in the ostrich

2017 ◽  
Author(s):  
Sukru Hakan Atalgin ◽  
Sevinc Ates ◽  
Ibrahim Kurtul ◽  
Hakan Terzi
Keyword(s):  
Alcian Blue ◽  
Bone Cells ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Double Staining ◽  
Alizarin Red ◽  
Struthio Camelus ◽  
Calcium Accumulation ◽  
Microscopic Features

This study documented macroscopic and microscopic features and mineralization of the caudally located tracheal rings and syrinx in two ostrich (struthio camelus) having three years of age. The syrinx and trachea of the birds were stained in toto with alcian blue and alizarin red for cartilage and mineralization. Observations on the syrinx and trachea, measurements and photography were performed under stereo-microscopy. They were stained grossly using alizarin red and alcian blue to visualize mineralization and histologically by hemotoxylin and eosin (HandE) to detect ossification areas, if any. Results revealed incomplete tracheal rings located caudally in-between the intact ones. Alizarin red and alcian blue staining displayed mineralized regions grossly. Histological slides by HandE staining showed no ossification. Overall results proposed that alizarin red and alcian blue double staining is a good toll to determine mineralization which is calcium accumulation in the tissue before the formation of bone cells.

scholarly journals STUDY OF VERTEBRAL MORPHOGENESIS OF COBIA LARVAE, (Rachycentron canadum) BY DOUBLE STAINING METHODS

2012 ◽  
Vol 7 (2) ◽  
pp. 105
Author(s):  
Afifah Nasukha ◽  
Titiek Aslianti ◽  
Agus Priyono
Keyword(s):  
Alcian Blue ◽  
Larval Stage ◽  
Blue Color ◽  
Double Staining ◽  
Alizarin Red ◽  
Rachycentron Canadum ◽  
Red Color ◽  
Organism Growth ◽  
Staining Methods ◽  
Vertebral Development

Vertebral development is one of the main indicators of organism growth. The aim of this study was to know the vertebral development of cobia Rachycentron canadum in larval stage (20 day post hatch). Vertebral assay was done with double staining methods. The result showed that cobia larvae from 0 dph up to 5 dph did not have cartilage. On 5 dph up to 10 dph had pre cartilage phase composed by calcium and on 10 dph up to 18 dph were cartilage phase and marked with blue color by alcian blue. Vertebral was formed perfectly as bones on 18 dph marked with red color by alizarin red. On 20 dph, cartilage had been fully transformed to bones, and the segment of vertebral was clearly formed. Measurement showed that length of cobia vertebrae was 20.20±3.90 mm, vertebrae segment was 0.91±0.11 mm and number of vertebral segments were between 25-26 segments.

scholarly journals Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 57-59
Author(s):  
Rakhmiyati Rakhmiyati ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Alcian Blue ◽  
Histochemical Staining ◽  
Alizarin Red S ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Tail Regeneration ◽  
Alizarin Red ◽  
Regenerate Tail ◽  
Hemidactylus Frenatus ◽  
Common House

Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.

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