Validation of Microbiological Assay Design of Neomycin Sulfate in 30 x 30 cm Rectangular Antibiotica Plate

Abstract A semiautomated microbiological folacin assay system is described. microcomputer A controls sample dilutions, medium addition, turbidity determination, and data acquisition. Assay capacity is 600 tubes per day, approximately twice that of comparable manual assays. Using the automated equipment, more samples can be compared within one assay, eliminating many sources of between-assay variation in large studies. Additional advantages of this sytem are reduced human errors, flexibility of assay design, and multifunctional component equipment. Folacin results from chicken liver, spinach, and breakfast cereal samples show equivalent precision for manual and automated assays.

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Microbiological Assay for Chloramphenicol Residues

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/68.5.1037 ◽

1985 ◽

Vol 68 (5) ◽

pp. 1037-1041 ◽

Cited By ~ 3

Author(s):

Carol J Singer ◽

Stanley E Katz

Keyword(s):

Muscle Tissue ◽

Minimal Medium ◽

Inhibitory Effect ◽

Microbiological Assay ◽

Bovine Urine ◽

Chicken Muscle ◽

Zones Of Inhibition ◽

Assay Design ◽

Urine And Serum ◽

Urine Serum

Abstract Procedures for the assay of chloramphenicol in milk, urine, serum, and muscle tissue are presented. The procedures specify an assay design with all standards as well as samples present on each plate, oxytetracycline in the buffer-diluent for greater sensitivity, a minimal medium to enhance the inhibitory effect of chloramphenicol on the assay organism, and a tetrazolium dye to improve the ability to measure the zones of inhibition. Recoveries of unbound chloramphenicol from bovine urine were 90.8%, from serum 88.3%, from milk 79.3%, from swine muscle 71.3%, and from beef and chicken muscle 61.0 and 61.4%, respectively. The lower levels of measurement in urine and serum were 0.25 ug/mL, 0.025 n-g/mL in milk, and 0.10 |xg/g in muscle tissue

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RELIABILITY CRITERIA FOR SATURATION ANALYSIS OF STEROIDS BY COMPETITIVE PROTEIN BINDING

Acta Endocrinologica ◽

10.1530/acta.0.065s061 ◽

1970 ◽

Vol 65 (1_Suppl) ◽

pp. S61-S78 ◽

Cited By ~ 3

Author(s):

Billy D. Reeves ◽

David W. Calhoun

Keyword(s):

Protein Binding ◽

Assay Method ◽

Statistical Control ◽

Protein Binding Assay ◽

Competitive Protein Binding Assay ◽

System 2 ◽

Competitive Protein Binding ◽

Single Method ◽

Assay Design ◽

Proper Perspective

ABSTRACT This communication is an attempt to delineate and define reliability criteria for saturation analysis of steroids by competitive protein binding assay. The discussion of these criteria evolved from three major considerations of assay method that help to place the ultimate criterion of accuracy in proper perspective. These major considerations are: 1) the measurement system, 2) the assay design and 3) the calculations and statistical control. Such an approach permits an evaluation, both relative and absolute, for a single method or for multiple methods.

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