Test Method Validation for Quantitative Determination of Heavy Metals in Olive Oils by Inductive Coupled Plasma (ICP – AES) using Low Cost and Simple Extraction Method

AbstractObjective: The purpose of this study was to develop a low-cost, yet sensitive and precise UHPLC method for the quantitative determination of ostarine from dietary supplements (DS) for athletes. The analytical performance of the method was verified on a DS legally acquired from a specialized website for athletes. The uniformity of mass and content of the ostarine DS was also verified.Methods: For the quantitative determination of ostarine a UHPLC method was developed and validated. The separation was performed using a reversed-phase C18 column, using a mixture of 75% methanol: 25% formic acid 0.1% in isocratic elution, at a flow rate of 0.5 ml/min. The uniformity of mass and content of DS was performed following the methodology described in the European Pharmacopoeia 7th Edition.Results: The validated method was specific and linear on the concentration range of 1-25 µg/ml and was precise and accurate at all concentration levels, according to the official guidelines for validating analytical methods. An average mass of 510 mg content was obtained for the ostarine capsules, with an RSD of 2.41%. Regarding the uniformity of the content, an average of 4.65 mg ostarine/capsule was obtained with an RSD of 1.05%.Conclusions: The developed UHPLC method was suitable, rapid, sensitive and allowed quantitative determination of active substance content in a DS with ostarine (92.91% ostarine/capsule from 5 mg ostarine/capsule declared by the manufacturer).

Download Full-text

The Determination of Chemical Forms of Heavy Metals in Shooting Area Contaminated Soil Using Sequential Extraction Method

Journal of Soil and Groundwater Environment ◽

10.7857/jsge.2015.20.6.111 ◽

2015 ◽

Vol 20 (6) ◽

pp. 111-116 ◽

Cited By ~ 1

Author(s):

Gyeonghye Moon ◽

Hongki Park ◽

Kyoungkeun Yoo ◽

Jha Manis Kumar ◽

Alorro Richad Diaz ◽

...

Keyword(s):

Heavy Metals ◽

Sequential Extraction ◽

Contaminated Soil ◽

Extraction Method ◽

Chemical Forms ◽

Sequential Extraction Method

Download Full-text

Quantitative determination of phenolic compounds using LC-DAD-ESI-MS/MS in cv. Ayvalik olive oils as affected by harvest time

Journal of Food Measurement & Characterization ◽

10.1007/s11694-016-9389-x ◽

2016 ◽

Vol 11 (1) ◽

pp. 226-235 ◽

Cited By ~ 10

Author(s):

Hasim Kelebek ◽

Serkan Selli ◽

Osman Kola

Keyword(s):

Phenolic Compounds ◽

Quantitative Determination ◽

Harvest Time ◽

Esi Ms ◽

Olive Oils

Download Full-text

SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM

Journal of the Chilean Chemical Society ◽

10.4067/s0717-97072019000104265 ◽

2019 ◽

Vol 64 (1) ◽

pp. 4265-4267

Author(s):

Sigrid Mennickent ◽

Marta De Diego ◽

Bernd Liser ◽

Lina Trujillo

Keyword(s):

Quantitative Determination ◽

Human Serum ◽

Extraction Method ◽

Liquid Extraction ◽

Liquid Liquid Extraction

Download Full-text

Spectrometric Determination of Heavy Metals Present in Mango Fruit of Nepali Origin

Amrit Research Journal ◽

10.3126/arj.v1i1.32457 ◽

2020 ◽

Vol 1 (1) ◽

pp. 72-77

Author(s):

Naresh Pant ◽

Anup Subedee ◽

Ram Bahadur Gharti ◽

Santu Shrestha

Keyword(s):

Heavy Metals ◽

Detection Limit ◽

Quantitative Determination ◽

Standard Method ◽

Random Sampling ◽

Sampling Method ◽

Spectrometric Method ◽

Mango Fruit ◽

Atomic Absorption Spectrometric

Quantitative determination of heavy metals; Fe, Zn, Co, Pb and Ni in Mango fruit of Nepali origin, locally sourced was carried out. Fifteen Mango samples were collected by random sampling method, converted into analyte sample by standard method and analyzed by using Atomic absorption spectrometric method. The amount of heavy metals Fe, Zn, Co and Ni present in sample was, 0.570±0.48, 0.510±0.031, 0.431±0.021, 0.106±0.003 mg/kg respectively. The results indicated the concentration of Zn & Co were higher (WHO 0.320 & 0.05), and the concentration of Nickel (Ni) was below the maximum permissible limit issued by WHO. The concentration of the Lead (Pb) was found below the detection limit of the instrument used.

Download Full-text