Fast quantitative determination of phenolic compounds in grape juice by UPLC-MS: method validation and characterization of juices produced with different grape varieties

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

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QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v12i1.30589 ◽

2019 ◽

Vol 12 (1) ◽

pp. 548

Author(s):

Ruben Ashotovich Pogosyan ◽

Olga Vladimirovna Nesterova ◽

Dmitry Olegovich Bokov ◽

Irina Alexandrovna Samylina

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Phenolic Compounds ◽

Quantitative Determination ◽

Gallic Acid ◽

Carboxylic Acids ◽

High Performance ◽

Fruit Pulp ◽

Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

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Physical, Chemical-physical and chemical characterization of must and integral juice in grape cultivars

Scientific Electronic Archives ◽

10.36560/1032017452 ◽

2017 ◽

Vol 10 (3) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

I. T. S. Viana ◽

G. V. A. Fonseca ◽

G. H. Bueno ◽

J. O. F. Melo

Keyword(s):

Chemical Characterization ◽

Grape Juice ◽

Titratable Acidity ◽

Mineral Elements ◽

Flame Emission ◽

Level Of Significance ◽

Physical And Chemical ◽

Grape Cultivars

In recent years there has been an important increase in the production of grape derivatives such as integral grape juice. Thus, it was intended with the accomplishment of this study to evaluate the composition of the grape juice of the grape cultivars: Bordô, Concord e Isabel in the composition of integral grape juice from the Bento Gonçalves-RS. The samples were evaluated for classical analysis:°Brix, pH, titratable acidity, ratio Brix / titratable acidity made through methods, physics, physical chemistry and chemistry. The mineral elements sodium and potassium analyzed by flame emission and color intensity by colorimetry. For the statistical analysis of the data, the Tukey test was applied at the 5% level of significance. The contents of integral grape juice evaluated, SS, A Tand minerals presented according to the legislation, the pH was within the comparative average with other works. The determination of the color demonstrated the need to make cuts.

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Quantitative determination of phenolic compounds using LC-DAD-ESI-MS/MS in cv. Ayvalik olive oils as affected by harvest time

Journal of Food Measurement & Characterization ◽

10.1007/s11694-016-9389-x ◽

2016 ◽

Vol 11 (1) ◽

pp. 226-235 ◽

Cited By ~ 10

Author(s):

Hasim Kelebek ◽

Serkan Selli ◽

Osman Kola

Keyword(s):

Phenolic Compounds ◽

Quantitative Determination ◽

Harvest Time ◽

Esi Ms ◽

Olive Oils

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In situ structure and force characterization of 2D nano-colloids at the air/water interface

Soft Matter ◽

10.1039/c9sm01476d ◽

2019 ◽

Vol 15 (42) ◽

pp. 8475-8482

Author(s):

Giovanni Li-Destri ◽

Roberta Ruffino ◽

Nunzio Tuccitto ◽

Giovanni Marletta

Keyword(s):

Quantitative Determination ◽

Experimental Method ◽

Water Interface ◽

Liquid Interfaces ◽

Interaction Forces ◽

Air Water Interface

We have developed a novel experimental method, which enables quantitative determination of interaction forces between interfacial nanoparticles as a function of the inter-particle distance at liquid interfaces.

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