Energy production and sanitation improvement using microbial fuel cells

2013 ◽  
Vol 3 (3) ◽  
pp. 383-391 ◽  
Author(s):  
I. Ieropoulos ◽  
J. Greenman ◽  
D. Lewis ◽  
O. Knoop

This study builds on the previous work of urine utilisation and uses small-scale microbial fuel cells (MFCs), working both as individual units in cascade or collectively as a stack, to utilise artificial urine. Artificial urine was prepared at concentrations typically found in real human urine with peptone employed as a surrogate proteinacious component. MFCs were constructed from Nanocure® polymer using rapid prototype technology. The anode and cathode electrodes were made of 15 cm2 carbon veil, folded down to fit in the 1 mL chambers. Eight MFCs were inoculated using activated anaerobic sludge; after 17 days of fed batch mode they were switched to continuous flow, initially at 0.09 mL/h and subsequently at 0.43 mL/h, resulting in HRT of 12.69 minutes/MFC. MFCs showed stable performance following the maturing period and produced, under polarisation experiments, peak power levels of 117 μW, corresponding to 962.94 W/m3. Continuous flow experiments data showed higher power production, increasing with the concentration of the carbon/energy source within artificial urine. The work demonstrates that artificial urine of varying composition can be successfully utilised for the production of energy and concomitant cleanup of organic waste. Finally, in line with the practical implementation and robotics work in our group, the small-scale MFCs were configured into a stack and directly energised electronic devices.

2017 ◽  
Vol 31 (6) ◽  
pp. 6132-6139 ◽  
Author(s):  
M. Amirul Islam ◽  
Baranitharan Ethiraj ◽  
Chin Kui Cheng ◽  
Abu Yousuf ◽  
Md. Maksudur Rahman Khan

1997 ◽  
Vol 52 (1) ◽  
pp. 110-116
Author(s):  
Michael Gerster ◽  
Martin Maier ◽  
Nils Clausen ◽  
Jens Schewitz ◽  
Ernst Bayer

Sulphurization is a crucial step during synthesis of phosphorothioate oligonucleotides. Insufficient reaction leads to inhomogeneous products with phosphodiester defects and subsequently to destabilization of the oligomers in biological media. To achieve a maximum extent of sulphur incorporation, various sulphurizing agents have been investigated. Solely, the use of Beaucage reagent provided satisfactory results on PS-PEG supports. Based on our investigations in small scale synthesis (1 μmol) with continuous-flow technique, upscaling to the 0.1-0.25 mmolar range has been achieved using a peptide synthesizer. The syntheses were performed in batch mode with standard phosphoramidite chemistry. Additionally, large scale synthesis of a phosphodiester oligonucleotide has been carried out on PS-PEG with optimized protocols and compared to small scale synthesis on different supports. Products were analysed by 31P NMR, capillary gel electrophoresis and electrospray mass spectrometry. An extent of sulphurization of 99% and coupling effiencies of more than 99% were obtained and the products proved to have similar purity compared to small scale syntheses on CPG


2012 ◽  
Vol 66 (4) ◽  
pp. 748-753 ◽  
Author(s):  
Jaecheul Yu ◽  
Younghyun Park ◽  
Haein Cho ◽  
Jieun Chun ◽  
Jiyun Seon ◽  
...  

Microbial fuel cells (MFCs) can convert chemical energy to electricity using microbes as catalysts and a variety of organic wastewaters as substrates. However, electron loss occurs when fermentable substrates are used because fermentation bacteria and methanogens are involved in electron flow from the substrates to electricity. In this study, MFCs using glucose (G-MFC), propionate (P-MFC), butyrate (B-MFC), acetate (A-MFC), and a mix (M-MFC, glucose:propionate:butyrate:acetate = 1:1:1:1) were operated in batch mode. The metabolites and microbial communities were analyzed. The current was the largest electron sink in M-, G-, B-, and A-MFCs; the initial chemical oxygen demands (CODini) involved in current production were 60.1% for M-MFC, 52.7% for G-MFC, 56.1% for B-MFC, and 68.3% for A-MFC. Most of the glucose was converted to propionate (40.6% of CODini) and acetate (21.4% of CODini) through lactate (80.3% of CODini) and butyrate (6.1% of CODini). However, an unknown source (62.0% of CODini) and the current (34.5% of CODini) were the largest and second-largest electron sinks in P-MFC. Methane gas was only detected at levels of more than 10% in G- and M-MFCs, meaning that electrochemically active bacteria (EAB) could out-compete acetoclastic methanogens. The microbial communities were different for fermentable and non-fermentable substrate-fed MFCs. Probably, bacteria related to Lactococcus spp. found in G-MFCs with fermentable substrates would be involved in both fermentation and electricity generation. Acinetobacter-like species, and Rhodobacter-like species detected in all the MFCs would be involved in oxidation of organic compounds and electricity generation.


2015 ◽  
Vol 37 (1) ◽  
pp. 114-120 ◽  
Author(s):  
Keiichi Kubota ◽  
Tomohide Watanabe ◽  
Takashi Yamaguchi ◽  
Kazuaki Syutsubo

2014 ◽  
Vol 535 ◽  
pp. 141-144
Author(s):  
Xiao Qin Zhao ◽  
Xiao Jie Sun

A single-chamber and membrane-less microbial fuel cells (MFC) was successfully started up using anaerobic sludge as inoculums without any nutrient elements for 20 d. Under 30 °C, excess sludge SS was about 21000 mg·L-1, anode area for 31.4 cm2and in 200 mM NaCl concentration agent conditions experiment MFC, while the control device (CD) directly with original sludge anaerobic digestion. The electricity generation of microbial fuel cell and the contrast of substrate changes were investigated. The results show that obtained maximum voltage is 597.3 mV, pH in MFC is slightly higher than in contrast test. Volume reduction in MFC is larger than the controls. Reducing sugar in MFC is lower than that in CDs. Proteins increase at first and then decrease, finally there is no significant difference in both of MFC and CD. Key words: Microbial Fuel Cells, Excess Sludge, Anaerobic Digestion, Reutilization


2010 ◽  
Vol 78 (1) ◽  
pp. 44-50 ◽  
Author(s):  
I. Ieropoulos ◽  
J. Greenman ◽  
C. Melhuish

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