scholarly journals Molecular detection of Toxoplasma gondii in natural surface water bodies in Poland

2018 ◽  
Vol 16 (4) ◽  
pp. 657-660 ◽  
Author(s):  
M. Adamska
Keyword(s):  
Surface Water ◽  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Water Bodies ◽  
Nested Polymerase Chain Reaction ◽  
Genotype I ◽  
Natural Surface ◽  
B1 Gene ◽  
Genotype Identification ◽  
Surface Water Bodies

Abstract The aim of this study was molecular detection of Toxoplasma gondii in 36 natural surface water bodies in Poland, including preliminary genotype identification and determination of co-occurrence of this parasite with other protozoa that have been detected in previous studies. The examined DNA samples were obtained before to detect Cryptosporidium, Giardia and free-living amoebae. Nested polymerase chain reaction (PCR) based on B1 gene and sequencing was performed for both confirmation of parasite presence in water and genotype identification. T. gondii DNA was detected in 19.4% (7/36) water bodies, while in the case of other studies, T. gondii prevalence ranged between 0% and over 56%. These differences may be caused by natural variations in T. gondii occurrence as well as different sample volumes and methods of sample processing or DNA isolation and detection. Two cases of double contamination were reported: T. gondii with Cryptosporidium parvum and T. gondii with potentially pathogenic Acanthamoeba T4 genotype, thus there is a possibility of mixed infection in humans after occasional contact with water. Obtained T. gondii strains were genetically identical or closely similar (99.8%) to RH strain representing genotype I, however, further examinations involving more loci will be conducted to identify the genotype.

scholarly journals Natural and artificial surface water bodies owned and used by forestry enterprises of Lviv region

2019 ◽  
Vol 53 ◽  
pp. 177-185
Author(s):  
Fedir Kiptach ◽  
Nataliya Blazhko
Keyword(s):  
Surface Water ◽  
Key Words ◽  
Water Bodies ◽  
Water Reservoirs ◽  
Natural Surface ◽  
Natural Streams ◽  
Artificial Surface ◽  
Surface Water Bodies ◽  
Artificial Water Bodies ◽  
Artificial Water

The areas of land with natural and artificial surface water bodies owned and used by forestry enterprises of administrative and territorial units of Lviv region are analyzed. Their classification according to the rating values of the described indicators is made, and schematic charts are drawn. The interval for determining the number of classification groups was calculated geometrically as the amplitude between the maximum and minimum values of the areas is too large. Forestry enterprises using water from natural and artificial surface water objects covering an area of 1,963.3 ha (including 1.5 % (642.1 ha) from natural streams (rivers and springs) and lakes and 3.1 % (1,321.2 ha) of artificial surface water bodies (canals, reservoirs and trenches, ponds and artificial water bodies) account for 12.9 % of the total area (42,781.6 ha) of lands of this category in the region. The largest areas of land with natural and artificial surface water bodies owned and used by forestry enterprises are recorded in Sokal (315 ha), Zhovkva (262.8), Busk (235.1), Skole (196.6), Brody (162.9), Mykolaiv (153), Drohobych (125.1), Radekhiv (120), Turka (118.7), and Kamyanka-Buzka (92.8 ha) districts, medium and moderate ones – in Staryi Sambir (68.3 ha) and Yavoriv (27 ha) districts, and the smallest ones – in Stryi (18 ha), Horodok (15), Zolochiv (15), Pustomyty (13), Sambir (7), Zhydachiv (6), Peremyshliany (4), and Mostyska (2.7 ha) districts and in Lviv (5.3 ha). The largest areas of land with natural surface water bodies owned and used by forestry enterprises are recorded in Skole (196.1 hа), Drohobych (124.6), Turka (118.7) and Staryi Sambir (65.8 hа) districts, considerable, medium and moderate ones – in Brody (29.8 hа), Zhovkva (25.5), Yavoriv (21), Zolochiv (13), Kamyanka-Buzka (11.7), Stryi (17) and Sambir (17 hа) districts, small, very small and too small – in Sokal (6.0 hа), Mostyska (2.7), Mykolaiv (2), Busk (1.9) and Zhydachiv (1 hа) districts and in Lviv (5.3 hа), in Horodok, Peremyshliany, Pustomyty and Radekhiv districts no such land has been recorded. The largest and considerable areas of land with artificial surface water bodies owned and used by forestry enterprises are recorded in Sokal (309.0 hа), Zhovkva (237.3), Busk (233.2), Mykolaiv (151), Brody (133.1), Radekhiv (120) and Kamyanka-Buzka (81.1 hа) districts, medium and moderate ones – in Horodok (15 hа), Pustomyty (13), Stryi (8), Yavoriv (6) and Zhydachiv (5 hа) districts, small, very small and too small – in Peremyshliany (4 hа), Staryi Sambir (2.5), Zolochiv (2), Skole (0.5) and Drohobych (0.5 hа) districts, in Mostyska, Sambir and Turka districts no such land has been recorded. Key words: surface water, streams, water reservoirs, forestry enterprises.

scholarly journals Catchments of German Surface Water Bodies

2021 ◽  
Author(s):  
Andreas Gericke ◽  
Judith Mahnkopf ◽  
Markus Venohr
Keyword(s):  
Surface Water ◽  
Water Bodies ◽  
Surface Water Bodies

On methodological approaches to the improvement of surface water bodies and target indicators of the natural waters’ quality

2021 ◽  
Vol 27 (1) ◽  
pp. 6-14
Author(s):  
V. Zaslonovsky ◽  
◽  
N. Sharapov ◽  
M. Bosov ◽  
◽  
...  
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
Natural Water ◽  
Aquatic Ecosystems ◽  
Natural Waters ◽  
Water Bodies ◽  
Surface Water Bodies ◽  
Methodological Approaches ◽  
Natural Water Bodies

The paper is devoted to the development of proposals concerning the improvement of surface water bodies and the appointment of target indicators of the natural waters’ quality. The paper considers a variety of problems of preserving, restoring and improving the quality of natural surface waters: from regional features of the formation of natural water composition, to differences in the requirements for the quality of water consumed. The object of the study is the qualitative indicators of the waters of natural water bodies, and the subject of the study is the methodological approaches to the normalization of these indicators. The purpose of the work is to identify the main shortcomings in the domestic system of rationing the quality of natural waters, and to form appropriate proposals. For this purpose, the following tasks were set and solved: to review the methodological approaches to assessing and rationing the quality of natural waters used in some foreign countries and in the Russian Federation, to identify the main causes and shortcomings; to make proposals for improving the current system of water quality rationing. The result of this work is the conclusions about the lack of impact of the strategy operating in Russia in the development of schemes of complex use and protection of water objects, standards of permissible impact on water bodies, the appointment of permissible discharge limits in terms of maintaining and improving the water quality of natural water bodies. The reasons that led to this are indicated. The necessity of taking into account, in addition to the physical and chemical composition, also hydrobiological parameters is shown. It is concluded that instead of fisheries management standards, environmental standards should serve as the basis for target indicators of water quality of natural water bodies, which determine the well-being of humans and the stable functioning of aquatic ecosystems, taking into account regional factors. Proposals were made to adjust the methodological approaches to the implementation of this strategy. According to the authors, these proposals will speed up the solution of the main objectives of the strategy – the ecological improvement of water bodies, the preservation of unique aquatic ecosystems and the environmentally safe development of territories that previously experienced relatively small anthropogenic impacts (Eastern Siberia and the Far East)

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