Fungi immobilization for landfill leachate treatment

2010 ◽  
Vol 62 (6) ◽  
pp. 1240-1247 ◽  
Author(s):  
Jenjira Saetang ◽  
Sandhya Babel
Keyword(s):  
Landfill Leachate ◽  
Corn Starch ◽  
White Rot Fungi ◽  
White Rot ◽  
Optimum Conditions ◽  
Treatment Efficiency ◽  
Leachate Treatment ◽  
Cod Reduction ◽  
Optimum Ph ◽  
Effects Of Ph

This paper investigated treatment of landfill leachate collected from Nonthaburi landfill site, Thailand, by using immobilized white rot fungi, namely, Trametes versicolor BCC 8725 and Flavodon flavus BCC 17421. Effects of pH and co-substrates were investigated at different contact times. Three types of co-substrates as carbon source used in this study are glucose, corn starch and cassava. Treatment efficiency was evaluated based on color, BOD, and COD removal. Initial BOD and COD were found to be 5,600 and 34,560 mg/L, respectively. The optimum pH was found to be 4, the optimum co-substrate concentration (glucose, corn starch and cassava) was 3 g/L and the optimum contact time was 10 days for both types of fungi. Addition of glucose, corn starch and cassava as co-substrate at optimum conditions could remove 78, 74, and 66% of color, respectively for T. versicolor and 73, 68, and 60%, respectively, for F. flavus. Moreover, for T. versicolor, BOD and COD reduction of 69 and 57%, respectively, could be achieved at optimum conditions when using glucose as a co-substrate. For F. flavus, BOD and COD reduction of 66 and 52%, respectively were obtained when using glucose as a co-substrate. White rot fungi can be considered potentially useful in the treatment of landfill leachate as they can help in removing color, BOD and COD due to their biodegradative abilities.

scholarly journals Effect of cellulose as co-substrate on old landfill leachate treatment using white-rot fungi

2017 ◽  
Vol 241 ◽  
pp. 1067-1076 ◽  
Author(s):  
A. Bardi ◽  
Q. Yuan ◽  
G. Siracusa ◽  
I. Chicca ◽  
M. Islam ◽  
...  
Keyword(s):  
Landfill Leachate ◽  
White Rot Fungi ◽  
White Rot ◽  
Leachate Treatment

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

scholarly journals Utilization of the White-rot Fungus, Trametes menziesiifor Landfill Leachate Treatment

2015 ◽  
Vol 44 (3) ◽  
pp. 309-316 ◽  
Author(s):  
W. A. R. Wan Razarinah ◽  
M. Noor Zalina ◽  
Noorlidah Abdullah
Keyword(s):  
Landfill Leachate ◽  
White Rot ◽  
White Rot Fungus ◽  
Leachate Treatment

Ultrasound as a pre-oxidation for biological landfill leachate treatment

2007 ◽  
Vol 55 (12) ◽  
pp. 175-179 ◽  
Author(s):  
E. Neczaj ◽  
M. Kacprzak
Keyword(s):  
Landfill Leachate ◽  
Irradiation Time ◽  
Ultrasound Irradiation ◽  
Ammonia Removal ◽  
Aerobic Treatment ◽  
Treatment Efficiency ◽  
Leachate Treatment ◽  
Field Frequency ◽  
Ultrasonic Pretreatment ◽  
Static Conditions

In this study, the effects of low energy ultrasound irradiation on landfill leachate treatment by means of sequencing bath reactor were investigated. The aim of this work was to estimate the influence of leachate irradiation time on aerobic treatment efficiency. The sonification of the leachate was carried out in static conditions using the disintegrator UD-20. The field frequency of 22 kHz (the power output equals to 180 W) and amplitude of 12 μm was applied. The sonification time was changed in the range of 30–140 s. It was found that ultrasonic pretreatment enhances the subsequent aerobic digestion resulting in a better degradation of landfill leachate. The sonification of raw leachate leads to enhancement of COD and ammonia removal as compare to experiment without ultrasound.

Effect of Culture Technique of Ganoderma Australe Mycelia on Percentage Removal of Leachate Organics

2016 ◽  
Vol 13 (1) ◽  
pp. 131
Author(s):  
Wan Razarinah Wan Abdul Razak ◽  
Noor Zalina Mahmood ◽  
Noorlidah Abdullah
Keyword(s):  
Landfill Leachate ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Culture Technique ◽  
Biological Oxygen Demand ◽  
White Rot ◽  
Toxic Substances ◽  
Percentage Removal ◽  
Ganoderma Australe ◽  
Biological Methods

Leachate (liquid pollutant), which is highly contaminated with organic matter and toxic substances is a major problem that arised from landfill. Biological methods have proven to be effective to remove organic matters that are abundant in leachate. This study is intended to compare the used of free mycelia and immobilized mycelia of the white-rot fungi, Ganoderma australe for the  removal of landfill leachate organics. The organics fraction of landfill leachate was measured by biological oxygen demand (BOD5), and chemical oxygen demand (COD). The experiment revealed that free mycelia of G. australe showed capability in removing leachate BOD5  but not COD. However, the use of immobilized G. australe displayed the best result in the removal of BOD5 and COD leachate after 4 weeks of treatment in flasks with 93.09% and 17.84% percentage removal of BOD5 and COD, respectively. Therefore, G. australe can be considered potentially useful in the treatment of landfill leachate as they can help in removing BOD and COD due to their biodegradative abilities.

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