Removal of Organic Matters in Landfill Leachate with White Rot Fungi

2015 ◽  
Vol 23 (5) ◽  
pp. 13-22
Author(s):  
Myoung Sun Jeong ◽  
Gi Eun Kim
Keyword(s):  
Landfill Leachate ◽  
White Rot Fungi ◽  
White Rot ◽  
Organic Matters

Fungi immobilization for landfill leachate treatment

2010 ◽  
Vol 62 (6) ◽  
pp. 1240-1247 ◽  
Author(s):  
Jenjira Saetang ◽  
Sandhya Babel
Keyword(s):  
Landfill Leachate ◽  
Corn Starch ◽  
White Rot Fungi ◽  
White Rot ◽  
Optimum Conditions ◽  
Treatment Efficiency ◽  
Leachate Treatment ◽  
Cod Reduction ◽  
Optimum Ph ◽  
Effects Of Ph

This paper investigated treatment of landfill leachate collected from Nonthaburi landfill site, Thailand, by using immobilized white rot fungi, namely, Trametes versicolor BCC 8725 and Flavodon flavus BCC 17421. Effects of pH and co-substrates were investigated at different contact times. Three types of co-substrates as carbon source used in this study are glucose, corn starch and cassava. Treatment efficiency was evaluated based on color, BOD, and COD removal. Initial BOD and COD were found to be 5,600 and 34,560 mg/L, respectively. The optimum pH was found to be 4, the optimum co-substrate concentration (glucose, corn starch and cassava) was 3 g/L and the optimum contact time was 10 days for both types of fungi. Addition of glucose, corn starch and cassava as co-substrate at optimum conditions could remove 78, 74, and 66% of color, respectively for T. versicolor and 73, 68, and 60%, respectively, for F. flavus. Moreover, for T. versicolor, BOD and COD reduction of 69 and 57%, respectively, could be achieved at optimum conditions when using glucose as a co-substrate. For F. flavus, BOD and COD reduction of 66 and 52%, respectively were obtained when using glucose as a co-substrate. White rot fungi can be considered potentially useful in the treatment of landfill leachate as they can help in removing color, BOD and COD due to their biodegradative abilities.

Effect of Culture Technique of Ganoderma Australe Mycelia on Percentage Removal of Leachate Organics

2016 ◽  
Vol 13 (1) ◽  
pp. 131
Author(s):  
Wan Razarinah Wan Abdul Razak ◽  
Noor Zalina Mahmood ◽  
Noorlidah Abdullah
Keyword(s):  
Landfill Leachate ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Culture Technique ◽  
Biological Oxygen Demand ◽  
White Rot ◽  
Toxic Substances ◽  
Percentage Removal ◽  
Ganoderma Australe ◽  
Biological Methods

Leachate (liquid pollutant), which is highly contaminated with organic matter and toxic substances is a major problem that arised from landfill. Biological methods have proven to be effective to remove organic matters that are abundant in leachate. This study is intended to compare the used of free mycelia and immobilized mycelia of the white-rot fungi, Ganoderma australe for the  removal of landfill leachate organics. The organics fraction of landfill leachate was measured by biological oxygen demand (BOD5), and chemical oxygen demand (COD). The experiment revealed that free mycelia of G. australe showed capability in removing leachate BOD5  but not COD. However, the use of immobilized G. australe displayed the best result in the removal of BOD5 and COD leachate after 4 weeks of treatment in flasks with 93.09% and 17.84% percentage removal of BOD5 and COD, respectively. Therefore, G. australe can be considered potentially useful in the treatment of landfill leachate as they can help in removing BOD and COD due to their biodegradative abilities.

Effect of Culture Technique of Ganoderma Australe Mycelia on Percentage Removal of Leachate Organics

2016 ◽  
Vol 13 (1) ◽  
pp. 131 ◽  
Author(s):  
Wan Razarinah Wan Abdul Razak ◽  
Noor Zalina Mahmood ◽  
Noorlidah Abdullah
Keyword(s):  
Landfill Leachate ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
Culture Technique ◽  
Biological Oxygen Demand ◽  
White Rot ◽  
Toxic Substances ◽  
Percentage Removal ◽  
Ganoderma Australe ◽  
Biological Methods

Leachate (liquid pollutant), which is highly contaminated with organic matter and toxic substances is a major problem that arised from landfill. Biological methods have proven to be effective to remove organic matters that are abundant in leachate. This study is intended to compare the used of free mycelia and immobilized mycelia of the white-rot fungi, Ganoderma australe for the  removal of landfill leachate organics. The organics fraction of landfill leachate was measured by biological oxygen demand (BOD5), and chemical oxygen demand (COD). The experiment revealed that free mycelia of G. australe showed capability in removing leachate BOD5  but not COD. However, the use of immobilized G. australe displayed the best result in the removal of BOD5 and COD leachate after 4 weeks of treatment in flasks with 93.09% and 17.84% percentage removal of BOD5 and COD, respectively. Therefore, G. australe can be considered potentially useful in the treatment of landfill leachate as they can help in removing BOD and COD due to their biodegradative abilities.

scholarly journals Effect of cellulose as co-substrate on old landfill leachate treatment using white-rot fungi

2017 ◽  
Vol 241 ◽  
pp. 1067-1076 ◽  
Author(s):  
A. Bardi ◽  
Q. Yuan ◽  
G. Siracusa ◽  
I. Chicca ◽  
M. Islam ◽  
...  
Keyword(s):  
Landfill Leachate ◽  
White Rot Fungi ◽  
White Rot ◽  
Leachate Treatment

OPTIMIZATION OF BIOMASS AND ENDO-B-1,4-GLUCANASE BY WHITE ROT FUNGI NATIVE FROM ARGENTINA

2017 ◽  
Vol 16 (11) ◽  
pp. 2581-2588
Author(s):  
Ernesto M. Giorgio ◽  
Maria I. Fonseca ◽  
Andrea L. Morales ◽  
Pedro D. Zapata ◽  
Laura L. Villalba
Keyword(s):  
White Rot Fungi ◽  
White Rot

The Onset of Lignin-Modifying Enzymes, Decrease of AOX and Color Removal by White-Rot Fungi Grown on Bleach Plant Effluents

1991 ◽  
Vol 24 (3-4) ◽  
pp. 189-198 ◽  
Author(s):  
V. P. Lankinen ◽  
M. M. Inkeröinen ◽  
J. Pellinen ◽  
A. I. Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Active Form ◽  
White Rot Fungi ◽  
Pulp Mill ◽  
Color Removal ◽  
Effluent Treatment ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Peroxidases ◽  
Pulp Mill Effluents

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.

Revalorizing Lignocellulose for the Production of Natural Pharmaceuticals and Other High Value Bioproducts

2019 ◽  
Vol 26 (14) ◽  
pp. 2475-2484 ◽  
Author(s):  
Congqiang Zhang ◽  
Heng-Phon Too
Keyword(s):  
Clostridium Thermocellum ◽  
White Rot Fungi ◽  
Brown Rot ◽  
Cost Effective ◽  
White Rot ◽  
Chemical Pretreatment ◽  
Significant Challenge ◽  
Drug Molecules ◽  
Natural Medicine ◽  
Renewable Natural Resource

Lignocellulose is the most abundant renewable natural resource on earth and has been successfully used for the production of biofuels. A significant challenge is to develop cost-effective, environmentally friendly and efficient processes for the conversion of lignocellulose materials into suitable substrates for biotransformation. A number of approaches have been explored to convert lignocellulose into sugars, e.g. combining chemical pretreatment and enzymatic hydrolysis. In nature, there are organisms that can transform the complex lignocellulose efficiently, such as wood-degrading fungi (brown rot and white rot fungi), bacteria (e.g. Clostridium thermocellum), arthropods (e.g. termite) and certain animals (e.g. ruminant). Here, we highlight recent case studies of the natural degraders and the mechanisms involved, providing new utilities in biotechnology. The sugars produced from such biotransformations can be used in metabolic engineering and synthetic biology for the complete biosynthesis of natural medicine. The unique opportunities in using lignocellulose directly to produce natural drug molecules with either using mushroom and/or ‘industrial workhorse’ organisms (Escherichia coli and Saccharomyces cerevisiae) will be discussed.

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