scholarly journals Density of Dermatophagoides spp. and Its Relationship with House-dust Mite Specific Serum IgE in Persistent Asthma

2020 ◽  
Vol 4 (2) ◽  
pp. 61
Author(s):  
Annisa Mulia Anasis ◽  
Anna Rozaliyani ◽  
Heri Wibowo
Keyword(s):  
House Dust ◽  
Immunoglobulin E ◽  
Persistent Asthma ◽  
Specific Ige ◽  
Bronchial Tree ◽  
House Dust Mites ◽  
Dust Mites ◽  
Serum Ige ◽  
Asthmatic Patients ◽  
Asthma Group

Background: Asthma is a chronic inflammation of the bronchial tree that emerges as a response to exogenous factors, such as allergens, irritants, and infections. Some asthmatic patients had been reported having symptoms of asthma due to house-dust mites (HDM) allergen exposure. It is associated with immune responses which were increased in the form of specific Immunoglobulin E (IgE) production against HDM allergens. This case-control study aimed to determine the HDM profiles in persistent asthmatic patients, including density of mites, as well as its relationship with specific IgE anti-HDM serum levels.Materials and Methods: A total of 13 patients with persistent asthma and 12 control patients had their specific anti-HDM IgE levels examined using Immulite 2000 xpi. The house dust samples were taken and analyzed with the Fain method.Results: The results have shown that 69% of patients in the persistent asthma group and 25% of normal patients were positive for IgE anti-HDM. Dermatophagoides pteronyssinus is a predominant species with a total of 120 mites (83.9%) of 143 mites. Correlation analysis indicated a positive relationship between IgE anti-HDM levels within the serums of patients and the density of mites in the dust obtained from bedroom spaces (Spearmen Rho, R=0.35, p=0.04).Conclusion: Positive IgE anti-HDM patients in the persistent asthma group were higher (69%) than those in the clinically normal group (25%). The density of mites were dominated by D. Pteronyssinus. The bedroom-dust mites density revealed a positive correlation with serum IgE anti-HDM levels in persistent asthma patients.Keywords: asthma, density, Dermatophagoides spp.,IgE

scholarly journals Could FeNO Predict Asthma in Patients with House Dust Mites Allergic Rhinitis?

Medicina ◽  
2020 ◽  
Vol 56 (5) ◽  
pp. 235
Author(s):  
Ioana Adriana Muntean ◽  
Ioana Corina Bocsan ◽  
Stefan Vesa ◽  
Nicolae Miron ◽  
Irena Nedelea ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Immunoglobulin E ◽  
Biological Evaluation ◽  
House Dust Mites ◽  
Dust Mites ◽  
Persistent Allergic Rhinitis ◽  
Tnf Α ◽  
One Year ◽  
High Level

Background and Objectives: The evolution of allergic rhinitis to asthma is a part of “atopic march”. The aim of this study was to analyze possible predictive markers for asthma occurrence in patients with allergic rhinitis to house dust mites (HDM). Materials and Methods: Fifty-eight patients with persistent allergic rhinitis (PAR) were included. The clinical, biological evaluation and fractionated exhaled nitric oxide (FeNO) measurement were performed at enrolment. The patients were clinically evaluated after one year to determine asthma occurrence. Results: The severity of rhinitis symptoms, levels of total immunoglobulin E (IgE), ICAM-1, VCAM-1, E-selectin and IL-6, but not IL-8 and TNF-α were higher in patients with allergic rhinitis who developed asthma compared to non-asthmatics, but the differences were not significant to considered them as predictive factors for asthma occurrence. The risk of asthma was independently influenced by patients aged over 30 years ((OR-3.74; CI95% 0.86–16.31; p = 0.07), a duration of allergic rhinitis over 12 months ((OR-4.20; CI95% 0.88–20; p = 0.07) and a basal FeNO over 28 parts per billion (pbb) ((OR-18.68; CI95% 3.79–92.05; p < 0.001). Conclusion: Clinical and biological parameters may predict asthma occurrence in patients with persistent allergic rhinitis to HDM. Adult patients with a longer duration of rhinitis symptoms and a high level of FeNO have a greater risk to develop asthma.

HOUSE DUST MITE ALLERGENS: FROM NATIVE EXTRACTS TO MODIFIED PREPARATIONS

2021 ◽  
Author(s):  
Anna Vasilyeva ◽  
Valentina M. Berzhets ◽  
Nina S. Petrova ◽  
Svetlana V. Khlgatian ◽  
Stanislava Yu. Petrova ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Binding Reaction ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Elisa Inhibition

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain

2008 ◽  
Vol 56 (2) ◽  
pp. 163-171 ◽  
Author(s):  
Ana Goicoa ◽  
Luciano Espino ◽  
Isabel Rodríguez ◽  
Anna Puigdemont ◽  
Pilar Brazis ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
House Dust ◽  
Immunoglobulin E ◽  
Geographic Area ◽  
Geographic Region ◽  
House Dust Mites ◽  
Dust Mites ◽  
Storage Mites ◽  
Canine Atopic Dermatitis

Sensitisation to mites is frequent in atopic dogs. The main mite genus involved in canine atopic dermatitis is Dermatophagoides . The importance of storage mite allergens in dogs has been controversial. The aim of this study was to evaluate the sensitisation rates against storage mites ( Lepidoglyphus destructor and Tyrophagus putrescentiae ) and house dust mites ( Dermatophagoides farinae and D. pteronyssinus ) in atopic dogs from Galicia, a highly humid and temperate region of Spain, using a FcɛRIα-based immunoglobulin E (IgE) in vitro test. The study was performed on 95 dogs suffering from atopic dermatitis and presenting detectable specific serum IgE levels: 91.6% of the dogs tested positive for storage mites, whereas sensitisation to house dust mites was detected in 87.4%. These results indicate the importance of storage mites in this specific geographic area.

scholarly journals Immunoglobulin G1 subclass responses can be used to detect specific allergy to the house dust mites Dermatophagoides farinae and Dermatophagoides pteronyssinus in atopic dogs

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
N. Khantavee ◽  
C. Chanthick ◽  
A. Tungtrongchitr ◽  
N. Techakriengkrai ◽  
S. Suradhat ◽  
...  
Keyword(s):  
House Dust ◽  
Characteristic Curve ◽  
False Positive Rate ◽  
Specific Ige ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Allergen Specific Immunotherapy ◽  
Intradermal Testing ◽  
Positive Rate

Abstract Background In dogs with atopic dermatitis, intradermal testing (IDT) or allergen specific IgE serological testing are routinely employed to identify causative allergens. These allergens can then be used for allergen-specific immunotherapy and allergy management. The clinical relevance of this testing is affected by the source of allergen, and other biomarkers that are more related to specific allergens still need to be identified. The aim of this study was to investigate levels of specific IgE, total IgG, and IgG1 and IgG2 subclasses against the local house dust mites (HDM) Dermatophagoides farinae (DF) and D. pteronyssinus (DP) as biomarkers by using in-house ELISAs in healthy (n = 33) and atopic dogs (AD) (n = 44) that were either positive or negative by IDT to HDM. Results Being over 3 years of age was a risk factor for AD (Odds Ratio (OD) = 4.10, 95% Confidence interval (CI) 1.57–10.75, p = 0.0049), but there was no relation to IDT outcomes (OR = 0.9091, 95% CI 0.22–3.74, p = 1.00). High levels of all antibody isotypes (IgE, IgG, IgG1 and IgG2) against HDM were found in aged healthy dogs (> 3 years old). In AD, HDM-IgE and IgG1 levels were higher in dogs that were IDT positive to HDM than in IDT negative animals. Levels of IgE and IgG1 could be used to distinguish the specific allergens, whereas total IgG and IgG2 levels were not different between IDT-positive and IDT-negative AD. By the receiver operating characteristic curve at a false-positive rate = 0.10, both IgE and IgG1 showed better sensitivity than IgG and IgG2. Similar to IgE, serum IgG1 concentration was also relevant to IDT outcomes. Conclusions Our in-house ELISAs coated with local HDM were useful for evaluating antibody levels, and we propose use of the HDM-specific IgG1 subclass as a biomarker to detect HDM specific allergens in AD, potentially together with an IgE based platform.

scholarly journals Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E Detection in Patients with Isolated Allergic Conjunctivitis

2021 ◽  
Vol 10 (5) ◽  
pp. 960
Author(s):  
Jung Yeob Han ◽  
Hun Lee ◽  
Jae Lim Chung ◽  
Young Jun Kim ◽  
Jae Yong Kim ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Conjunctivitis ◽  
Immunoglobulin E ◽  
Age Groups ◽  
Specific Ige ◽  
House Dust Mites ◽  
Detection Rates ◽  
Immunoblot Assay ◽  
Simultaneous Test ◽  
Positive Rate

We aimed to investigate the immunoglobulin E (IgE) detection rate and allergen patterns in patients with isolated allergic conjunctivitis using the multiple allergen simultaneous test (MAST)-immunoblot assay. A total of 120 patients with allergic conjunctivitis and no associated rhinitis, asthma, or dermatitis underwent the MAST-immunoblot assay to measure serum total IgE (TIgE) and serum specific IgE (SIgE) against 57 allergens. Patients were classified into subgroups based on the season when the eye symptoms were exacerbated, and TIgE and SIgE positivity. Differences between sex and age groups were also analyzed. Of the 120 patients, 57.5% (69 patients) and 69.2% (83 patients) were positive for TIgE (≥100 IU/mL) and SIgE (≥0.7 IU/mL), respectively. The allergens that most frequently triggered sensitization in the study population were Dermatophagoides farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Alternaria, and house dust. House dust mites, such as D. farinae and D. pteronyssinus, showed the highest detection rates regardless of the season. Men had a higher positive rate for TIgE than women, whereas a higher rate of sensitization, detected as SIgE positivity, was seen in younger patients. In conclusion, MAST-immunoblot assay can detect sensitizing allergens in patients with isolated allergic conjunctivitis.

scholarly journals Allergic rhinitis complicated by hypertrophy of the mucous membrane of nasal turbinates in patients of Northern Vietnam

2020 ◽  
Vol 7 (6) ◽  
pp. 3813-3818
Author(s):  
Kryukov Andrei Ivanovich ◽  
Galina Petrovna Bondareva ◽  
Nguyen Thi Phuong Thao
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Cavity ◽  
Mucous Membrane ◽  
House Dust ◽  
Inferior Turbinate ◽  
Specific Ige ◽  
House Dust Mites ◽  
Moderate Degree ◽  
Dust Mites ◽  
Northern Vietnam

Introduction: Constant contact with allergens contributes to the formation of allergic rhinitis and swelling of the mucous membrane of the nasal cavity. Moreover, constant inflammation stimulates hypertrophy of the mucous membrane of the nasal turbinates and paranasal sinuses. This study aimed to assess the clinical features of hypertrophic rhinitis (HR) in combination with allergic rhinitis (AR) in patients from Northern Vietnam to improve the effectiveness of treatment and quality of life of patients with this pathology of the nasal cavity. Methods: The study was conducted from June to September 2018 by the Department of Otorhinolaryngology, Thai Nguyen Central General Hospital, Vietnam. A total of 158 patients with symptoms of chronic rhinitis, aged 5–70 years, were examined. All patients with allergic rhinitis were subjected to standard, specific allergological tests, such as the determination of specific IgE in the blood, and statistics were assessed according to the otorhinolaryngology examination and CT scan. All patients were also examined by otolaryngologists. Results: According to the results of our study, among 64 patients with a diagnosis of AR, a total of 45 (70.31%) patients were diagnosed as AR in combination with a hypertrophy of the mucous membrane of the nasal turbinate, with an average duration of morbidity of 5.8 ±1.6 years. Moreover, 17 (37.78%) patients were diagnosed with severe hypertrophy of the mucous membrane of the nasal inferior turbinate (the nasal inferior turbinate is 50% or more of nasal cavity and with nasal polyps). The sensitization to house dust mites and fungi was high. Conclusion: The peculiar findings of AR in combination with HR detected in patients of Northern Vietnam was associated with the onset of the disease at the age of 20-40 years, the predominance in men, the predominance of the disease in smokers, and the predominance in residents of cities and industrial areas (71.1%). Indeed, there was an observed combination of AR with severe and moderate degree of HR. The sensitization of patients with AR in combination with HR in Northern Vietnam are similar to those in Asia, with predominant allergy to house dust mites.

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