Primary Standards Laboratory report 1st half 1993

The article contains the results of the metrological examination and research of the accuracy indicators of a method for diagnosing aircraft gas turbine engines of the D30KU/KP family using an ultra-high-frequency plasma complex. The results of metrological examination of a complete set of regulatory documents related to the diagnostic methodology, and an analysis of the state of metrological support are provided as well. During the metrological examination, the traceability of a measuring instrument (diagnostics) – an ultrahigh-frequency plasma complex – is evaluated based on the scintillation analyzer SAM-DT-01–2. To achieve that, local verification schemes from the state primary standards of the corresponding types of measurements were built. The implementation of measures to eliminate inconsistencies identified during metrological examination allows to reduce to an acceptable level the metrological risks of adverse situations when carrying out aviation activities in industry and air transportation. In addition, the probability of occurrence of errors of the first and second kind in the technological processes of tribodiagnostics of aviation gas turbine engines is reduced when implementing a method that has passed metrological examination in real practice. At the same time, the error in determining ratings and wear indicators provides acceptable accuracy indicators and sufficient reliability in assessing the technical condition of friction units of the D-30KP/KP2/KU/KU-154 aircraft engines.

Characterization and Quantification of Selenoprotein P: Challenges to Mass Spectrometry

International Journal of Molecular Sciences ◽

10.3390/ijms22126283 ◽

2021 ◽

Vol 22 (12) ◽

pp. 6283

Author(s):

Jérémy Lamarche ◽

Luisa Ronga ◽

Joanna Szpunar ◽

Ryszard Lobinski

Keyword(s):

Mass Spectrometry ◽

State Of The Art ◽

Animal Health ◽

Primary Standard ◽

Selenoprotein P ◽

Primary Standards ◽

Analytical Approaches ◽

Careful Investigation ◽

Accurate Quantification

Selenoprotein P (SELENOP) is an emerging marker of the nutritional status of selenium and of various diseases, however, its chemical characteristics still need to be investigated and methods for its accurate quantitation improved. SELENOP is unique among selenoproteins, as it contains multiple genetically encoded SeCys residues, whereas all the other characterized selenoproteins contain just one. SELENOP occurs in the form of multiple isoforms, truncated species and post-translationally modified variants which are relatively poorly characterized. The accurate quantification of SELENOP is contingent on the availability of specific primary standards and reference methods. Before recombinant SELENOP becomes available to be used as a primary standard, careful investigation of the characteristics of the SELENOP measured by electrospray MS and strict control of the recoveries at the various steps of the analytical procedures are strongly recommended. This review critically discusses the state-of-the-art of analytical approaches to the characterization and quantification of SELENOP. While immunoassays remain the standard for the determination of human and animal health status, because of their speed and simplicity, mass spectrometry techniques offer many attractive and complementary features that are highlighted and critically evaluated.