scholarly journals Flow cytometric analysis of mitotic cycle perturbation by chemical carcinogens in cultured epithelial cells. [Effects of benzo(a)pyrene-diol-epoxide on mitotic cycle of cultural mouse liver epithelial cells]

1978 ◽  
Author(s):  
Andrew Leonard Pearlman
Keyword(s):  
Epithelial Cells ◽  
Mouse Liver ◽  
Mitotic Cycle ◽  
Flow Cytometric Analysis ◽  
Chemical Carcinogens ◽  
Liver Epithelial Cells ◽  
Flow Cytometric ◽  
Diol Epoxide ◽  
Cultured Epithelial Cells

scholarly journals Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.

1993 ◽  
Vol 90 (11) ◽  
pp. 5123-5127 ◽  
Author(s):  
A. M. Pfeifer ◽  
K. E. Cole ◽  
D. T. Smoot ◽  
A. Weston ◽  
J. D. Groopman ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Tumor Antigen ◽  
Human Liver ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Chemical Carcinogens ◽  
Large Tumor ◽  
Liver Epithelial Cells ◽  
Normal Human Liver ◽  
Normal Human

scholarly journals CD31 Expression Defines Heterogeneity of Donor Plasmacytoid Dendritic Cell Populations That Home to the Thymus: Implications for Control of GvHD

Blood ◽  
2018 ◽  
Vol 132 (Supplement 1) ◽  
pp. 3325-3325
Author(s):  
Alina Ulezko Antonova ◽  
Yitong Wang ◽  
Mojibade Hassan ◽  
Yiwen Li ◽  
Edmund K. Waller
Keyword(s):  
Bone Marrow ◽  
T Cells ◽  
Epithelial Cells ◽  
Research Funding ◽  
Flow Cytometric Analysis ◽  
Thymic Epithelial Cells ◽  
Specific Marker ◽  
Flow Cytometric ◽  
Ccr9 Expression ◽  
Cd31 Expression

Abstract Introduction: Plasmacytoid dendritic cells (pDC) are known to possess tolerogenic properties in allogeneic bone marrow transplantation (allo-BMT), but the relationship between pDC lineage and their modulation of graft-versus-host disease (GvHD) has not been defined. Recently, we have shown that murine treatment with the pleiotropic cytokine FMS-like Tyrosine Kinase 3 Ligand (Flt3L) increases pDC content in the marrow, and that allo-BMT of Flt3L-treated marrow (FBM) grafts leads to higher overall survival and reduced GvHD in lethally irradiated hosts. Furthermore, FBM pDC have increased potency in preventing GvHD on a cell-by-cell basis (Hassan, EBMT, 2018). Interestingly, FBM pDC expressed CD11b, a myeloid-specific marker that is not expressed on currently defined pDC. Lineage ontogeny of pDC can be tracked based upon exclusive expression of CD31 and Ly6C on myeloid-progenitor derived pDC, while pDC from lymphoid progenitors express RAG1 and Siglec H. We hypothesized that treatment of murine bone marrow donors with Flt3L, which enhances their GvHD-reducing activity, modifies the distribution of lineage-specific precursors of pDC in bone marrow, and that lineage-associated pDC will have distinct biological activity in allo-BMT. Methods and Results: To assess the effect of Flt3L on BM pDC, we treated C57BL/6 mice with Flt3L (CDX-301, 300 ug/kg) on days -1 and -4 relative to bone marrow harvest. Using flow cytometric analysis, we show that FBM pDC over-express myeloid-specific markers CD31, Ly6C and CD11b, but down-regulate the lymphoid-specific marker Siglec H (Figure 1). Moreover, we observed similar phenotypic trends for myeloid-specific markers in pDC from the marrow of RAG1KO mice, confirming the existence of a unique myeloid-derived population of pDC. To determine the effect of pDC linage on their gene expression, we performed Illumina RNA Sequencing on human-derived pDC from Flt3L-mobilized peripheral blood and from human bone marrow. Interestingly, we found that Flt3L-treated pDC overexpress PRSS16, which is known to be exclusively expressed on cortical thymic epithelial cells. Furthermore, using GFP-transgenic mice as a source of donor pDC in B6 -> B10.BR allo-BMT, we show via confocal microscopy that donor pDC selectively home to the recipient thymus (Figure 2). Moreover, flow cytometric analysis revealed that homing of FBM pDC to the thymus is not impaired, in spite of decreased CCR9 expression on FBM pDC in comparison to BM pDC. Because it is currently believed that CCR9 expression on pDC is necessary for their homing to the thymus, these observations denote that FBM pDC use a CCR9-independent homing strategy. Since CD31 expression is upregulated in FBM pDC with enhanced immune-regulatory activity in allo-BMT models, these data suggest that local expression of CD31 by donor pDC in the thymic microenvironment may be relevant to their ability to favorably regulate thymopoiesis and limit the development of alloreactive T cells. Conclusions: Our data poses a link between pDC lineage and control of post-transplant GvHD, possibly via regulation of positive and negative selection of donor-derived T cells in the thymus. Specifically, we identify a CD31+ myeloid-specific population of pDC that emerges in the marrow upon treatment with Flt3L and exhibits a genetic profile that resembles thymic epithelial cells as a candidate for adoptive cell therapy to prevent GvHD. Disclosures Waller: Pharmacyclics: Other: Travel Expenses, EHA, Research Funding; Novartis Pharmaceuticals Corporation: Consultancy, Membership on an entity's Board of Directors or advisory committees, Research Funding; Kalytera: Consultancy; Celldex: Research Funding; Cambium Medical Technologies: Consultancy, Equity Ownership.

scholarly journals Flow Cytometric Analysis of DNA Content of Mouse Liver Cells Following in vivo Infection by Human Adenovirus Type 5

1981 ◽  
Vol 57 (1) ◽  
pp. 221-226 ◽  
Author(s):  
J. D. Murray ◽  
M. L. Berger ◽  
I. W. Taylor
Keyword(s):  
Dna Content ◽  
Mouse Liver ◽  
Flow Cytometric Analysis ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Liver Cells ◽  
Flow Cytometric ◽  
Adenovirus Type 5

scholarly journals Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis

Eye ◽  
2006 ◽  
Vol 21 (8) ◽  
pp. 1062-1066 ◽  
Author(s):  
M Mrugacz ◽  
J Zak ◽  
A Bakunowicz-Lazarczyk ◽  
J Wysocka ◽  
A Minarowska
Keyword(s):  
Cystic Fibrosis ◽  
Epithelial Cells ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Hla Dr ◽  
Conjunctival Epithelial Cells

Cytological studies on induced mitogynogenesis in Japanese flounder Paralichthys olivaceus (Temminck et Schlegel)

Zygote ◽  
2016 ◽  
Vol 24 (5) ◽  
pp. 700-706 ◽  
Author(s):  
Jilun Hou ◽  
Guixing Wang ◽  
Xiaoyan Zhang ◽  
Haijin Liu
Keyword(s):  
Mitotic Cycle ◽  
Paralichthys Olivaceus ◽  
Flow Cytometric Analysis ◽  
Japanese Flounder ◽  
Sea Bream ◽  
Cell Cleavage ◽  
Flow Cytometric ◽  
Monopolar Spindle ◽  
Effect Of Hydrostatic Pressure ◽  
Dense Chromatin

SummaryThe effect of hydrostatic pressure treatment on the induction of mitogynogenesis in the eggs of Japanese flounder Paralichthys olivaceus (Temminck et Schlegel) by using heterospecific sperm were studied. Before treatment, the eggs were at metaphase of the first mitosis. The spindle was disassembled by the treatment and then resembled in its pretreatment position, and the chromosomes were rearranged, i.e., the first mitosis was not blocked. During the second mitotic cycle, only a monopolar spindle was assembled in each blastomere and the chromosomes doubled, but cell cleavage was blocked. In the third cycle, mitosis proceeded normally with a bipolar spindle in each blastomere. Flow cytometric analysis of ploidy demonstrated that mitogynogenetic larvae were all diploid. The ultraviolet light-irradiated sperm of the red sea bream (Pagrus major) was condensed, formed a dense chromatin body, and randomly entered one blastomere.

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