scholarly journals In Vitro Growth of Lens Epithelial Cells from Cataract Patients - Association with Possible Risk Factors for Posterior Capsule Opacification

2014 ◽  
Vol 8 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Karin Sundelin ◽  
Anne Petersen ◽  
Yalda Soltanpour ◽  
Madeleine Zetterberg
Keyword(s):  
Risk Factors ◽  
Epithelial Cells ◽  
Cataract Surgery ◽  
Calf Serum ◽  
Posterior Capsule Opacification ◽  
Posterior Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Proliferative Capacity

Aim : Inter-individual differences in intrinsic proliferative capacity of lens epithelial cells may have importance for the risk of developing posterior capsule opacification (PCO) after cataract surgery. The purpose of the present study was to determine growth of human lens epithelial cells (HLEC) in culture and investigate possible associations with clinical characteristics of the donors, such as age, sex, pseudoexfoliation, uveitis and diabetes. Methods : Pieces of lens capsule and adhering lens epithelial cells were obtained through capsulorhexis at cataract surgery. Specimens were cultured in a humidified CO2-incubator using standard culture medium and 5% fetal calf serum for two weeks after which cultured cells were stained with carboxy-fluorescein diacetate succinimidyl ester. Image processing software was used to determine the area of the confluent epithelial cell layer in relation to the size of the original capsule specimen. Results : The increase in area of confluent HLEC showed a negative correlation with diabetes at the first week after surgery. Lower age and female sex showed border-line significant associations with a higher rate of cell proliferation. The presence of pseudoexfoliation in vivo did not significantly affect cell growth in culture postoperatively. Nor did installation of xylocain in the anterior chamber during surgery. Conclusion : Diabetes is associated with lower rate of proliferation of lens epithelial cells in culture. The lack of strong correlations between in vitro growth and known risk factors for PCO in the donors suggest that other factors than the proliferative capacity of the cells per se are important for PCO formation.

Surface modification of intraocular lenses with hyaluronic acid and lysozyme for the prevention of endophthalmitis and posterior capsule opacification

RSC Advances ◽  
2015 ◽  
Vol 5 (5) ◽  
pp. 3597-3604 ◽  
Author(s):  
Bailiang Wang ◽  
Quankui Lin ◽  
Tingwei Jin ◽  
Chenghui Shen ◽  
Junmei Tang ◽  
...  
Keyword(s):  
Surface Modification ◽  
Hyaluronic Acid ◽  
Epithelial Cells ◽  
Cataract Surgery ◽  
Posterior Capsule Opacification ◽  
Intraocular Lenses ◽  
Posterior Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells

Posterior capsule opacification is one of the complications of cataract surgery caused by the adhesion and reproduction of residual human lens epithelial cells (HLECs) on the posterior capsule.

Retrovirus-mediated transfer of a suicide gene into lens epithelial cells in vitro and in an experimental model of posterior capsule opacification

1999 ◽  
Vol 19 (6) ◽  
pp. 472-482 ◽  
Author(s):  
Bettina C. Couderc ◽  
Sylvie de Neuville ◽  
Victorine Douin-Echinard ◽  
Brigitte Serres ◽  
Stéphane Manenti ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Experimental Model ◽  
Suicide Gene ◽  
Posterior Capsule Opacification ◽  
Posterior Capsule ◽  
Lens Epithelial Cells

scholarly journals Metformin Reduced Collagen Deposition and Contractility, but Increased Collagen Degradation in in vitro Posterior Capsule Opacification Model

2021 ◽  
Vol 13 (2) ◽  
pp. 186-91
Author(s):  
Frisma Sagara Brilliyanto ◽  
Gatut Suhendro ◽  
Indri Wahyuni ◽  
Maftucah Rochmanti ◽  
Windhu Pramono
Keyword(s):  
Cataract Surgery ◽  
Posterior Capsule Opacification ◽  
Collagen Degradation ◽  
Posterior Capsule ◽  
Human Lens ◽  
Control Group ◽  
Collagen Deposition ◽  
Cell Contractility ◽  
Anterior Lens Capsule

BACKGROUND: Posterior capsule opacification (PCO) often occurs after cataract surgery. Metformin has been known to have an ability to inhibit fibrosis. This study aimed to investigate the effects of metformin on cell contractility, collagen deposition and degradation in human lens epithelial cells (HLEC) of cataract patients.METHODS: HLEC were isolated from the anterior lens capsule of patients undergoing cataract surgery. The HLEC culture was carried out using explant culture technique. The in vitro PCO model was created by scratching technique on HLEC cultures. The treatment groups were given 0.1, 0.5 and 1 mM metformin, respectively, while the control group were given 10% fetal bovine serum (FBS). On the 7th day after scratching technique, the collagen deposition, collagen degradation and cell contractility were evaluated.RESULTS: Collagen deposition in HLEC was significantly reduced after given 0.1 mM, 0.5 mM and 1 mM metformin (17.92±6.16 mg/mL, 12.92±4.31 mg/mL, 11.25±5.30 mg/mL, respectively), compared to the control group (31.46±7.52 μg/mL, p=0.002). Collagen degradation significantly was increased in the 0.1 mM, 0.5 mM and 1 mM metformin groups (4.77±9.27 mg/mL, 6.59±1.16 mg/mL, 6.35±1.90 mg/mL, respectively) compared to the control group (2.21±2.78 μg/mL, p=0.002). While, collagen contractility in HLEC was significantly reduced in 0.1mM, 0.5mM and 1 mM metformin groups (16.39±3.89%, 13.89±2.59%, 11.93±2.44%, respectively), compared to the control group (44.25±4.95%, p=0.000).CONCLUSION: Metformin reduced collagen deposition and contractility, but increased collagen degradation in HLEC of cataract patients through mechanism of extracellular matrix remodeling.KEYWORDS: metformin, human lens epithelial cell, fibrosis

scholarly journals P 229 Growth of human lens epithelial cells on the anterior lens capsule in vitro

1995 ◽  
Vol 35 ◽  
pp. S199
Author(s):  
J.H. Meyer ◽  
J. Schmidt ◽  
F. Eppinger ◽  
B. Flügel ◽  
K.U. Löffler ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lens Capsule ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Anterior Lens Capsule

scholarly journals On the Anticataractogenic Effects of L-Carnosine: Is It Best Described as an Antioxidant, Metal-Chelating Agent or Glycation Inhibitor?

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Hamdy Abdelkader ◽  
Michael Longman ◽  
Raid G. Alany ◽  
Barbara Pierscionek
Keyword(s):  
Epithelial Cells ◽  
Antioxidant Activities ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Metal Chelating ◽  
Human Lens Epithelial Cells

Purpose.L-Carnosine is a naturally occurring dipeptide which recently gained popularity as an anticataractogenic agent due to its purported antioxidant activities. There is a paucity of research and conclusive evidence to support such claims. This work offers compelling data that help clarify the mechanism(s) behind the anticataract properties of L-carnosine.Methods.Direct in vitro antioxidant free radical scavenging properties were assayed using three different antioxidant (TEAC, CUPRAC, and DPPH) assays. Indirect in vitro and ex vivo antioxidant assays were studied by measuring glutathione bleaching capacity and total sulfhydryl (SH) capacity of bovine lens homogenates as well as hydrogen-peroxide-stress assay using human lens epithelial cells. Whole porcine lenses were incubated in high galactose media to study the anticataract effects of L-carnosine. MTT cytotoxicity assays were conducted on human lens epithelial cells.Results.The results showed that L-carnosine is a highly potent antiglycating agent but with weak metal chelating and antioxidant properties. There were no significant decreases in lens epithelial cell viability compared to negative controls. Whole porcine lenses incubated in high galactose media and treated with 20 mM L-carnosine showed a dramatic inhibition of advanced glycation end product formation as evidenced by NBT and boronate affinity chromatography assays.Conclusion.L-Carnosine offers prospects for investigating new methods of treatment for diabetic cataract and any diseases that are caused by glycation.

scholarly journals p-Coumaric Acid Protects Human Lens Epithelial Cells against Oxidative Stress-Induced Apoptosis by MAPK Signaling

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Jiao Peng ◽  
Ting-ting Zheng ◽  
Yue Liang ◽  
Li-fang Duan ◽  
Yao-dong Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Mapk Signaling ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Dependent Manner ◽  
Coumaric Acid ◽  
Underlying Mechanisms ◽  
Induced Apoptosis

To protect against oxidative stress-induced apoptosis in lens epithelial cells is a potential strategy in preventing cataract formation. The present study aimed at studying the protective effect and underlying mechanisms of p-coumaric acid (p-CA) on hydrogen peroxide- (H2O2-) induced apoptosis in human lens epithelial (HLE) cells (SRA 01–04). Cells were pretreated with p-CA at a concentration of 3, 10, and 30 μM before the treatment of H2O2 (275 μM). Results showed that pretreatment with p-CA significantly protected against H2O2-induced cell death in a dose-dependent manner, as well as downregulating the expressions of both cleaved caspase-3 and cleaved caspase-9 in HLE cells. Moreover, p-CA also greatly suppressed H2O2-induced intracellular ROS production and mitochondrial membrane potential loss and elevated the activities of T-SOD, CAT, and GSH-Px of H2O2-treated cells. As well, in vitro study showed that p-CA also suppressed H2O2-induced phosphorylation of p-38, ERK, and JNK in HLE cells. These findings demonstrate that p-CA suppresses H2O2-induced HLE cell apoptosis through modulating MAPK signaling pathways and suggest that p-CA has a potential therapeutic role in the prevention of cataract.

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