AbstractAncylostoma tubaeformemay infect canids, felids and humans, and pose a potential risk to public health. Polymerase chain reaction (PCR) techniques were used to amplify the complete mitochondrial (mt) genome sequence ofA. tubaeformefrom cats and to analyse its sequence characteristics after molecular identification based on the internal transcribed spacer ITS1+ sequence. The results show that the complete mt genome sequence (GenBank accession number KY070315) ofA. tubaeformefrom cats was 13,730 bp in length, including 12 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, two non-coding regions and an AT-rich region. The nucleotide content of A and T was 77.93%, biased toward A and T. Twelve protein-coding genes used ATT, TTG and GTG as initiation codons, and TAA, TAG, TA and T as termination codons. The length of the 22 tRNA genes ranged from 52 to 62 bp, their predicted secondary structures were D loops and V loops. The lengths of the two rRNAs were 958 and 697 bp. Phylogenetic analyses showed thatA. tubaeformefrom cats was in the lineage ofAncylostoma, having a close phylogenetic relationship withA. caninum. This study reports for the first time the mt genome ofA. tubaeformefrom cats in China, which could enhance the mt genome database of Ancylostomatidae nematodes, and it offers the scientific basis for further studies in the genetic diversity of hookworms among different hosts.
Draft Genome Sequence of a Fermenting Bacterium, “Sphaerochaeta halotolerans” 4-11T, from a Low-Temperature Petroleum Reservoir in Russia
