Radiation safety is an action taken to protect workers, community members, and the environment from radiation hazards. The purpose of writing is to realize the best practice of radiation safety culture at Hermina Hospital Karawang. This descriptive study conducted interviews, observations, discussions, and direct measurements from 2020 to 2021. Using the Thermoluminescence Dosemeter (TLD) badge for radiation workers in 2020, 80% of all radiation workers used the TLD badge, and 20% did not because officers forgot and limited use when working in the covid room area. Radiation danger signs have been posted at all examination doors and radiology registration windows. Radiologists always turn on the lights every time they do an X-ray examination. All radiology employees at Hermina Hospital have performed routine health checks at least once a year. The test results of the apron pass fit for use; there is no visible damage in the form of tears, waves, or other damage. The results of the radiation exposure test in the conventional radiology room in the operator's room were 0.063µSv/h, behind the operator's door 0.143 Sv/h, in the administration room 0.087 Sv/h, at the radiology entrance 0.057 Sv/h, the medical gas room and parking area of 0.029 Sv/h and 0.08 Sv/h. The results of radiation exposure test in the area for the CT Scan, namely in the operator's room of 0.046 Sv/h, behind the operator's door 0.118 Sv/h, the entrance to the CT Scan room 0.204 Sv/h, in the poly room and parking area of 0.125 Sv/h, and 0.041 Sv/ h. The results of the exposure test were declared safe from radiation leakage. Radiation Protection Officer (RPO) Hermina Karawang Hospital has participated in online PPR requalification in 2021. The local Diagnostic Reference Level (DRL) value of Hermina Karawang Hospital for non-contrast head CT, namely with CTDIvol = 38.26 mGy and DLP = 777.29 mGy.cm. The implementation of radiation safety best practices at Hermina Hospital has been carried out routinely following applicable regulations regarding radiation safety. It takes consistency and high discipline for radiation workers and hospital management in maintaining the quality of hospital radiation safety. Keywords: Radiation safety, best practice, Diagnostic Reference Level (DRL)