scholarly journals Aktivitas Antioksidan Minuman Daun Beluntas Teh Hitam (Pluchea indica Less-Camelia sinensis)

2018 ◽  
Vol 38 (2) ◽  
pp. 200
Author(s):  
Paini Sri Widyawati ◽  
Tarsisius Dwi Wibawa Budianta ◽  
Yesiana Dwi Wahyu Werdani ◽  
Maria Olivia Halim
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Black Tea ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Iron Ion

The research was conducted to explore the potency of pluchea leaves-black tea drink as antioxidant at various proportions. The research used a single factor randomized block design of pluchea leaves and black tea proportions, including 100:0; 75:25; 50:50; 25:75; and 0:100% (w/w). Each of it was repeated five times. The parameters observed in this study were secondary metabolites, total phenolic, total flavonoids, free radical DPPH scavenging activity, and iron reducing power. The data were statistically analyzed using Analysis of Variance (ANOVA) at α = 5%, if the analysis showed a significant effect then it was continued with Duncan's Multiple Range Test (DMRT). Data was stated as mean ± standard deviation. The results showed that the secondary metabolites containing in drink from pluchea leaves and black tea at various proportions were alkaloids, flavonoids, phenolics, saponins, tannins, and cardiac glycosides. The increasing of black tea proportion in samples added the intensity of alkaloids, phenolics, flavonoids, saponins, and cardiac glycosides compounds detected, but the tannins were decreased. These secondary metabolites were correlated with total phenolic content (TPC) and total flavonoid content (TFC). The increasing of black tea proportion in drink significantly decreased DPPH free radical scavenging activity and iron ion reducing power, except for 100% black tea proportion. Tannin compounds seems determining antioxidant activity.  Based on coefficient correlation between TPC or TFC and DPPH free radical scavenging activity or iron ion reducing power, the result showed that DPPH free radical scavenging activity was dominantly contributed by TPC and iron ion reducing power was determined by TPC and TFC. ABSTRAKPenelitian ini dilakukan untuk mengetahui potensi minuman daun beluntas teh hitam sebagai antioksidan pada berbagai proporsi. Penelitian ini menggunakan rancangan acak kelompok satu faktor, yaitu proporsi daun beluntas teh hitam meliputi 100:0; 75:25; 50:50; 25:75; dan 0:100% (b/b). Tiap faktor diulang sebanyak 5 kali. Parameter yang diamati pada penelitian ini meliputi kandungan metabolit sekunder, total fenolik (TPC), total flavonoid (TFC), kemampuan menangkal radikal bebas DPPH, dan kemampuan mereduksi ion besi. Data dianalisis secara statistik dengan Analysis of Variance (ANOVA) pada α = 5%, jika terdapat beda signifikan dilanjutkan dengan uji Duncan's Multiple Range Test (DMRT). Data dinyatakan dengan rata-rata ± standar deviasi.  Hasil menunjukkan bahwa metabolit sekunder yang terkandung dalam minuman daun beluntas teh hitam pada berbagai proporsi adalah alkaloid, flavonoid, fenolik, saponin, tannin, dan kardiak glikosida. Peningkatan proporsi teh hitam menambah intensitas senyawa alkaloid, fenolik, flavonoid, saponin, dan kardiak glikosida yang terdeteksi, tetapi kandungan senyawa tannin berkurang. Kandungan metabolit sekunder ini berkorelasi dengan total fenolik (TPC) dan total flavonoid (TFC). Peningkatan proporsi teh hitam dalam minuman menurunkan kemampuan menangkal radikal bebas DPPH dan kemampuan mereduksi ion besi, kecuali pada proporsi teh hitam 100%. Kandungan senyawa tannin dalam minuman menentukan aktivitas antioksidan. Berdasarkan koefisien korelasi antara TPC atau TFC dan kemampuan menangkal radikal bebas DPPH atau kemampuan mereduksi ion besi, hasil menunjukkan bahwa kemampuan menangkal radikal bebas DPPH dominan dikontribusi oleh TPC dan kemampuan mereduksi ion besi ditentukan oleh TPC dan TFC.

scholarly journals Optimization of a Process for a Microgreen and Fruit Based Ready to Serve Beverage

2021 ◽  
pp. 41-56
Author(s):  
Anjali Sharma ◽  
Prasad Rasane ◽  
Anirban Dey ◽  
Jyoti Singh ◽  
Sawinder Kaur ◽  
...  
Keyword(s):  
Free Radical ◽  
Bioactive Compounds ◽  
Spinacia Oleracea ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Metal Chelation

A process for preparation of a microgreen and fruit based beverage was optimized using spinach (Spinacia oleracea) microgreen, pomegranate (Punica granatum), pineapple (Ananascomosus) and sugar. The blended juice in different ratios was analysed for total soluble solids, viscosity, sedimentation, acidity, metal chelation activity, free radical scavenging activity and reducing power. The optimized beverage had, 17.26 mL 100 mL-1 spinach microgreen juice, 57.07 mL 100 mL-1 pomegranate juice, 1.01 g 100 g-1 sugar and 24.66 mL 100 mL-1 pineapple juice. The product was high in nutrients, particularly protein, minerals (sodium, potassium and iron), vitamins (vitamin C), bioactive compounds (total phenols and total carotenoids) and had high antioxidant activity (metal chelation activity, free radical scavenging activity and reducing power). The antioxidants and bioactive compounds present in juice were designed to help reduce oxidative stress during inflammatory cases such as arthritis.

Heterogeneous Method for Grafting of Syringaldehyde onto Chitosan by Laccase Oxidation

2013 ◽  
Vol 477-478 ◽  
pp. 1340-1344
Author(s):  
Cheng Hua Yu ◽  
Ji Cheng Pei ◽  
Fang Dong Zhang
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Amino Groups

Heterogeneous method for grafting of syringaldehyde (SD) onto chitosan by laccase oxidation, yielding a product with improved antioxidant activities. The results showed that about 1.1% amino groups participated in this reaction. The FTIR showed that the absorption of amino groups were decreased, the absorption of benzene ring structures were disappeared and a new band appeared at 1637 cm-1 corresponding to C=N vibrations that were due to the Schiff base reaction between the chitosan and syringaldehyde. Additionally, the chitosan had insignificant reducing power and DPPH free radical scavenging activity, SD-chitosan had reducing power of A700=0.684, DPPH free radical scavenging activity of 53.2%. SD-chitosan presented improved antioxidant properties.

scholarly journals Aktivitas Penangkalan Radikal Bebas dan Kemampuan Reduksi Ekstrak Kulit Kayu Akway (Drimys piperita Hook. f.)

2019 ◽  
Vol 7 (4) ◽  
Author(s):  
Gino Nemesio Cepeda ◽  
Meike Meilan Lisangan ◽  
Mathelda Kurniaty Roreng ◽  
Elva Intan Permatasari ◽  
Dolly Citra Manalu ◽  
...  
Keyword(s):  
Free Radical ◽  
Vitamin C ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenol ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Akway (Drimys piperita Hook. f) merupakan tumbuhan yang termasuk dalam kelompok tumbuhan berkayu, berdaun tebal aromatik dan termasuk kerabat winteraceae. Tumbuhan ini dimanfaatkan sebagai obat tradisional untuk mengobati malaria dan untuk meningkatkan vitalitas tubuh. Beberapa penelitian kandungan fitokimia ekstrak akway telah dilakukan untuk mengetahui potensi bioaktivitas akway. Ekstrak kulit kayu akway dilaporkan mengandung kelompok senyawa alkaloid, saponin, tanin, flavonoid, terpenoid dan glikosida. Minyak atsiri kulit kayu akway mengandung linalool, β-pinen, α-pinen, nerolidol dan terpineol. Senyawa-senyawa tersebut dilaporkan memiliki aktivitas antioksidan yang tinggi. Tujuan penelitian ini adalah untuk menentukan kapasitas antioksidan ekstrak kulit kayu akway secara in vitro dan vitamin C sebagai kontrol positif. Pengujian kapasitas antioksidan yang dilakukan meliputi kandungan total fenol dengan metode Folin-Ciocalteu, kandungan flavonoid dengan metode aluminum klorida, kapasitas penangkalan radikal bebas menggunakan metode DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging assay dan daya reduksi menggunakan metode reduksi Fe+3 menjadi Fe+2. Hasil penelitian menunjukkan bahwa ekstrak metanol memiliki kandungan total fenol dan flavonoid yang tertinggi dibandingkan dengan ekstrak etanol dan etilasetat. Kandungan total fenol dan flavonoid ekstrak metanol masing-masing sebesar 18,22 dan 14,32%. Ekstrak metanol dan vitamin C memiliki kapasitas menangkal radikal bebas DPPH dan daya reduksi yang paling tinggi kemudian diikuti ekstrak etanol dan etilasetat. Kemampuan menangkal radikal bebas ekstrak metanol dan vitamin C pada konsentrasi 200 µg/ml masing-masing sebesar 90% dan 88,31% sedangkan daya reduksi masing-masing sebesar 0,54 dan 0,62. Kesimpulannya, ekstrak metanol memiliki kapasitas antioksidan yang paling tinggi dibandingkan dengan ekstrak etanol dan etilasetat. Abstract Free Radical Scavenging Activity and Reducing Power of Akway (Drimys piperita Hook. f.) Bark ExtractsAkway (Drimys piperita Hook. f) was a woody and aromatic plant of winteraceae. This plant was used as traditional medical plant to heal malaria and to enhance vitality of body. Some studies were done to know bioactivity potency of akway extracts. D. piperita bark extract contains alkaloid, saponin, tanin, flavonoid, terpenoid and glycoside. The bark essential oil of the plant consists of lynalool, β-pinene, α-pinene, nerolidol and terpineol. Those compounds were exhibited high antioxidant activity. The objectives of this research were to determine total phenol and flavonoid of the extracts and its antioxidant capacity which was compared to antioxidant capacity of vitamin C. The assay of antioxidant capacity comprised of total phenol and flavonoid content, free radical scavenging activity, and reducing power. Total phenol and flavonoid was determined using Folin-Ciocalteu and aluminum chloride method, respectively while determination of free radical scavenging activity and reducing power using DPPH-radical scavenging and Fe+3 to Fe+2 reducing power method, respectively. The results indicated that methanol extract had the highest in total phenol and flavonoid content. Total phenol and flavonoid of methanol extract were 18.22% and 14.32%, respectively. Vitamin C and methanol extract had the highest DPPH (1,1-Diphenyl-2-picrylhydrazyl)-radical scavenging activity and reducing power, respectively. DPPH-radical scavenging activity of methanol extract and vitamin C in concentration of 200 µg/ml were 90% and 88.31%, respectively while its reducing power were 0.54 and 0,62, respectively. As conclusion, methanol extract had the highest antioxidant activity compared with ethanol and ethyl acetate extracts.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (8) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (08) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
Siham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Quercetin as a Protease (á-Chymotrypsin) Inhibitor

2014 ◽  
Vol 14 (2) ◽  
pp. 91-96
Author(s):  
GB Bajracharya ◽  
DB Thapa
Keyword(s):  
Free Radical ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inhibitory Effect ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Chymotrypsin Inhibitor

Quercetin, isolated from the skin of red onion bulb, was methylated to afford quercetin 3,3’,4’,7-tetramethyl ether in 83.3% yield. Quercetin has displayed α-chymotrypsin inhibitory effect (IC50 = 88.9 ± 2.5 ìg/ml), DPPH free radical scavenging activity (IC50 = 14.87 ìg/ml) and ferric reducing power (EC1 = 112.94 ìM/l equivalent to 1 mM/l FeSO4.7H2O), whereas no enhancement of these biological activities was observed using its tetramethyl ether derivative. DOI: http://dx.doi.org/10.3126/njst.v14i2.10420   Nepal Journal of Science and Technology Vol. 14, No. 2 (2013) 91-96

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