scholarly journals Detection of species substitution in raw, cooked, and processed meats utilizing multiplex-PCR assay

2021 ◽  
Vol 26 (3) ◽  
pp. 128
Author(s):  
Muhammad Cahyadi ◽  
Nur Aini Dyah Fauzıah ◽  
Imam Tubagus Suwarto ◽  
Waraporn Boonsupthip
Keyword(s):  
Multiplex Pcr ◽  
Cytochrome B ◽  
Meat Products ◽  
Cytochrome B Gene ◽  
Processed Meat ◽  
Pcr Assay ◽  
Processed Meats ◽  
Multiplex Pcr Assay ◽  
Species Substitution ◽  
Meat Samples

The rise of beef consumption in Indonesia opens an opportunity for “rogue” suppliers to mix beef with other meat species that are relatively cheaper, such as pork, chicken, etc. The aim of this study was to identify pig and chicken meat in raw, cooked, and processed meat products using multiplex-PCR of mitochondrial DNA Cytochrome b gene, which is maternally inherited and widely used for forensic studies. A total of 90 samples-33 raw meats, 33 cooked meats, and 24 meatballs-were used in this study. Each sample was extracted to obtain the DNA genome and this was then amplified using multiplex-PCR. The PCR products were visualized using 2% agarose gel electrophoresis. The results showed that species contained in raw, cooked, and processed meat samples could be identified as indicated by DNA bands at 398, 274, 227, and 157 bp for pig, cattle, chicken, and goat species respectively. This study concluded that species substitution in raw, cooked, and processed meats could be detected using the Cytochrome b gene as a genetic marker through multiplex-PCR assay.

DEVELOPMENT OF MULTIPLEX PCR ASSAY FOR MEAT PRODUCTS AUTHENTICATION: TARGETING DOUBLE GENE

2019 ◽  
Vol 5 (2) ◽  
pp. 149-158
Author(s):  
M. A. Motalib Hossain ◽  
◽  
Syed Muhammad Kamal Uddin ◽  
Sharmin Quazi Bonny ◽  
Mohd Rafie Johan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Meat Products ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Fraud identification in industrial meat products by multiplex PCR assay

Food Control ◽  
2009 ◽  
Vol 20 (8) ◽  
pp. 696-699 ◽  
Author(s):  
S. Ghovvati ◽  
M.R. Nassiri ◽  
S.Z. Mirhoseini ◽  
A. Heravi Moussavi ◽  
A. Javadmanesh
Keyword(s):  
Multiplex Pcr ◽  
Meat Products ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

scholarly journals A Multiplex PCR Assay for the Detection of Food-borne Pathogens in Meat Products

2010 ◽  
Vol 30 (4) ◽  
pp. 590-596 ◽  
Author(s):  
Hyoun-Wook Kim ◽  
Ji-Hyun Kim ◽  
Seong-Ryul Rhim ◽  
Kyung-A Lee ◽  
Cheon-Jei Kim ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Meat Products ◽  
Pcr Assay ◽  
Food Borne Pathogens ◽  
Multiplex Pcr Assay ◽  
Food Borne

scholarly journals Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products

2017 ◽  
Vol 61 (1) ◽  
pp. 53-58
Author(s):  
Dalia Hamza ◽  
Sohad Dorgham ◽  
Ashraf Hakim
Keyword(s):  
Clostridium Perfringens ◽  
Meat Products ◽  
Type A ◽  
Chicken Meat ◽  
Pcr Assay ◽  
Type D ◽  
Multiplex Pcr Assay ◽  
Meat Samples ◽  
Potential Public Health

Abstract Introduction: The toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined. Material and Methods: Two hundred processed chicken meat samples from luncheon meats, nuggets, burgers, and sausages were screened for Clostridium perfringens by multiplex PCR assay for the presence of alpha (cpa), beta (cpb), epsilon (etx), iota (ia), and enterotoxin toxin (cpe) genes. The C. perfringens isolates were examined in vitro against eight antibiotics (streptomycin, amoxicillin, ampicillin, ciprofloxacin, lincomycin, cefotaxime, rifampicin, and trimethoprim-sulfamethoxazole) Results: An overall of 32 C. perfringens strains (16%) were isolated from 200 processed chicken meat samples tested. The prevalence of C. perfringens was significantly dependent on the type of toxin genes detected (P = 0.0), being the highest in sausages (32%), followed by luncheon meats (24%), burgers (6%), and nuggets (2%). C. perfringens type A was the most frequently present toxinotype (24/32; 75%), followed by type D (21.9 %) and type E (3.1%). Of the 32 C. perfringens strains tested, only 9 (28%) were enterotoxin gene carriers, with most representing type A (n = 6). C. perfringens strains differed in their resistance/susceptibility to commonly used antibiotics. Most of the strains tested were sensitive to ampicillin (97%) and amoxicillin (94%), with 100% of the strains being resistant to streptomycin and lincomycin. It is noteworthy that the nine isolates with enterotoxigenic potential had a higher resistance than the non-enterotoxigenic ones. Conclusion: The considerably high C. perfringens isolation rates from processed chicken meat samples and resistance to some of the commonly used antibiotics indicate a potential public health risk. Recent information about the isolation of enterotoxigenic C. perfringens type E from chicken sausage has been reported.

Usefulness of a Multiplex PCR for Detection of Diarrheagenic Escherichia coli in a Diagnostic Microbiology Laboratory Setting

2016 ◽  
Vol 1 (2) ◽  
pp. 38-42 ◽  
Author(s):  
Khairun Nessa ◽  
Dilruba Ahmed ◽  
Johirul Islam ◽  
FM Lutful Kabir ◽  
M Anowar Hossain
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Clinical Laboratory ◽  
Proteinase K ◽  
Microbiology Laboratory ◽  
Pcr Assay ◽  
E Coli ◽  
Diarrheagenic Escherichia Coli ◽  
Multiplex Pcr Assay ◽  
Diagnostic Microbiology

A multiplex PCR assay was evaluated for diagnosis of diarrheagenic Escherichia coli in stool samples of patients with diarrhoea submitted to a diagnostic microbiology laboratory. Two procedures of DNA template preparationproteinase K buffer method and the boiling method were evaluated to examine isolates of E. coli from 150 selected diarrhoeal cases. By proteinase K buffer method, 119 strains (79.3%) of E. coli were characterized to various categories by their genes that included 55.5% enteroaggregative E. coli (EAEC), 18.5% enterotoxigenic E. coli (ETEC), 1.7% enteropathogenic E. coli (EPEC), and 0.8% Shiga toxin-producing E. coli (STEC). Although boiling method was less time consuming (<24 hrs) and less costly (<8.0 US $/ per test) but was less efficient in typing E. coli compared to proteinase K method (41.3% vs. 79.3% ; p<0.001). The sensitivity and specificity of boiling method compared to proteinase K method was 48.7% and 87.1% while the positive and negative predictive value was 93.5% and 30.7%, respectively. The majority of pathogenic E. coli were detected in children (78.0%) under five years age with 53.3% under one year, and 68.7% of the children were male. Children under 5 years age were frequently infected with EAEC (71.6%) compared to ETEC (24.3%), EPEC (2.7%) and STEC (1.4%). The multiplex PCR assay could be effectively used as a rapid diagnostic tool for characterization of diarrheagenic E. coli using a single reaction tube in the clinical laboratory setting.Bangladesh J Med Microbiol 2007; 01 (02): 38-42

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