Detection of AtRKD4 Protein During Induction of Somatic Embryogenesis in Dendrobium lineale Rolfe Transgenic Orchids Carrying 35S::GR::AtRKD4

Dendrobium lineale is an Indonesian native orchid from the Spatulata section in Orchidaceae Family. This orchid is important because it is usually used as a parental plant in orchid breeding and is predicted to have a potential phytochemistry compound. In addition, in their natural habitat, this orchid is threatened due to forest exploitation and natural disaster. Therefore the precision mass propagation techniques for this orchid need to be conducted. Biotechnological approaches through inserting embryo gene such as AtRKD4 from Arabidopsis thaliana has already been successfully conducted. This study aims to check the integration stability of T-DNA harboring 35S::GR::AtRKD4 from ten selection transformants and to detect the existence of AtRKD4 protein after induction by Dexamethasone and/ Thidiazuron. The result showed that T-DNA were stably integrated into the genome of D. lineale transformants and the AtRKD4 protein with a molecular weight of 28.53 kDa was detected in D. lineale transformant plants after being induced by 15 µM DEX and 3 mgL-1 TDZ for 5 days.

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Somatic Embryogenesis for Mass Propagation of Ericaceae – A Case Study with Leucopogon verticillatus

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1023/b:ticu.0000007285.73884.fc ◽

2004 ◽

Vol 76 (2) ◽

pp. 137-146 ◽

Cited By ~ 6

Author(s):

J.M. Anthony ◽

T. Senaratna ◽

K.W. Dixon ◽

K. Sivasithamparam

Keyword(s):

Somatic Embryogenesis ◽

Mass Propagation

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EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v17n1.2016.p9-16 ◽

2017 ◽

Vol 17 (1) ◽

pp. 9

Author(s):

Yosi Zendra Joni ◽

Riry Prihatini ◽

Darda Efendi ◽

Ika Roostika

Keyword(s):

Somatic Embryogenesis ◽

Plant Growth ◽

Embryogenic Callus ◽

Plant Growth Regulator ◽

Somatic Embryos ◽

Casein Hydrolysate ◽

Malt Extract ◽

Mass Propagation ◽

Embryogenic Callus Induction

Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.

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Factors Influencing Somatic Embryogenesis Induction and Plant Regeneration with Particular Reference to Arabidopsis thaliana (L.) Heynh

Plant Growth Regulation ◽

10.1023/b:grow.0000038275.29262.fb ◽

2004 ◽

Vol 43 (1) ◽

pp. 27-47 ◽

Cited By ~ 177

Author(s):

Malgorzata D. Gaj

Keyword(s):

Arabidopsis Thaliana ◽

Somatic Embryogenesis ◽

Plant Regeneration ◽

Factors Influencing

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Somatic Embryogenesis in Arabidopsis Thaliana L

Somatic Embryogenesis and Synthetic Seed II - Biotechnology in Agriculture and Forestry ◽

10.1007/978-3-642-78643-3_30 ◽

1995 ◽

pp. 371-384 ◽

Cited By ~ 1

Author(s):

B. Huang ◽

M. M. Yeoman

Keyword(s):

Arabidopsis Thaliana ◽

Somatic Embryogenesis

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Propagation and Conservation of Native Forest Genetic Resources of Medicinal Use by Means of In Vitro and ex vitro Techniques

Natural Product Communications ◽

10.1177/1934578x1100600715 ◽

2011 ◽

Vol 6 (7) ◽

pp. 1934578X1100600

Author(s):

Sandra Sharry ◽

Marina Adema ◽

María A. Basiglio Cordal ◽

Blanca Villarreal ◽

Noelia Nikoloff ◽

...

Keyword(s):

Somatic Embryogenesis ◽

Vegetative Propagation ◽

Native Species ◽

Natural Habitat ◽

Folk Medicine ◽

Native Forest ◽

Indirect Organogenesis ◽

Acacia Caven ◽

Medicinal Use

In Argentina, there are numerous native species which are an important source of natural products and which are traditionally used in medicinal applications. Some of these species are going through an intense extraction process in their natural habitat which may affect their genetic diversity. The aim of this study was to establish vegetative propagation systems for three native forestal species of medicinal interest. This will allow the rapid obtainment of plants to preserve the germplasm. This study included the following species which are widely used in folk medicine and its applications: Erythrina crista-galli or “seibo” (astringent, used for its cicatrizant properties and for bronchiolitic problems); Acacia caven or “espinillo” (antirheumatic, digestive, diuretic and with cicatrizant properties) and Salix humboldtiana or “sauce criollo” (antipyretic, sedative, antispasmodic, astringent). The methodology included the micropropagation of seibo, macro and micropropagation of Salix humboldtiana and the somatic embryogenesis of Acacia caven. The protocol for seibo regeneration was adjusted from nodal sections of seedlings which were obtained from seeds germinated in vitro. The macropropagation through rooted cuttings of “sauce criollo” was achieved and complete plants of this same species were obtained through both direct and indirect organogenesis using in vitro cultures. The somatic embryogenesis for Acacia caven was optimized and this led to obtain a high percentage of embryos in different stages of development. We are able to support the conservation of native forest resources of medicinal use by means of vegetative propagation techniques.

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Somatic embryogenesis in cultured immature zygotic embryos and leaf protoplasts of Arabidopsis thaliana ecotypes

Planta ◽

10.1007/s004250050141 ◽

1997 ◽

Vol 202 (3) ◽

pp. 387-396 ◽

Cited By ~ 49

Author(s):

Yingkun Luo ◽

Hans-Ulrich Koop

Keyword(s):

Arabidopsis Thaliana ◽

Somatic Embryogenesis ◽

Zygotic Embryos ◽

Immature Zygotic Embryos ◽

Leaf Protoplasts

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Arabidopsis thaliana AtGpp1 and AtGpp2: two novel low molecular weight phosphatases involved in plant glycerol metabolism

Plant Molecular Biology ◽

10.1007/s11103-006-9104-0 ◽

2006 ◽

Vol 63 (4) ◽

pp. 505-517 ◽

Cited By ~ 17

Author(s):

José Antonio Caparrós-Martín ◽

Sonja Reiland ◽

Karl Köchert ◽

Mari Cruz Cutanda ◽

Francisco A. Culiáñez-Macià

Keyword(s):

Molecular Weight ◽

Arabidopsis Thaliana ◽

Glycerol Metabolism ◽

Low Molecular Weight

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REGENERATION OF Pimpinella pruatjan THROUGH SOMATIC EMBRYOGENESIS

Indonesian Journal of Agricultural Science ◽

10.21082/ijas.v8n2.2007.60-66 ◽

2016 ◽

Vol 8 (2) ◽

pp. 60

Author(s):

I. Roostika ◽

R. Purnamaningsih ◽

I. Darwati ◽

I. Mariska

Keyword(s):

Somatic Embryogenesis ◽

Embryo Development ◽

Callus Formation ◽

Natural Habitat ◽

Casein Hydrolysate ◽

Shoot Multiplication ◽

Dry Weight ◽

Embryogenic Calli ◽

The Media ◽

Medicinal Properties

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesian endangered plant which has various medicinal properties such as aphrodisiac, diuretic, and tonic. The plant is commonly harvested from its natural habitat, therefore it becomes endangered. Regeneration of pruatjan through organogenesis has been studied, but its shoot multiplication was very low (5 shoots per explant). The study aimed to investigate the best regeneration technique of pruatjan through somatic embryogenesis. This research was conducted at the tissue culture laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development in 2004-2005. Callus formation of pruatjan was induced from the petioles and leaves in Driver and Kuniyaki’s (DKW) based medium containing 2,4-D combined with picloram at the level of 0.5, 1.0, 1.5, and 1.5 ppm. Embryogenic calli were then transferred into embryo development medium in two ways. First, they were directly transferred into media containing IBA/NAA at the level of 0.5, 1.0, and 1.5 ppm. Second, they were indirectly transferred into media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate prior to the IBA/ NAA media. Parameters evaluated were fresh weight, dry weight, time initiation of embryogenic callus formation, and total number of embryos. The result showed that calli of pruatjan were successfully induced from the petioles and leaves. The best calli were induced from the leaves in the DKW medium containing 2.0 ppm 2,4-D and 0.5 ppm picloram. Embryo development of the calli was best if they were first grown in the media containing 2.0 ppm 2,4-D and 0.3% casein hydrolysate then transferred to the IBA/NAA media. The total number of somatic embryos was counted up to 103 on the medium containing 1.5 ppm IBA. This study indicated that pruatjan somatic embryogenesis regeneration required three different media, i.e. for callus induction, development and maturation, and for germination.

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Somatic Embryogenesis and Subsequent Plant Regeneration from Zygotic Embryo Derived Callus of Rubber (Hevea brasiliensis Muell. Arg).

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v27i1.35012 ◽

2017 ◽

Vol 27 (1) ◽

pp. 51-61 ◽

Cited By ~ 1

Author(s):

Md Mahbubur Rahman ◽

Maziah Mahmood ◽

Norhani Abdullah ◽

Noor Azmi Shaharuddin ◽

Waheeda Parvin

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Plant Growth ◽

Growth Regulators ◽

Hevea Brasiliensis ◽

Zygotic Embryo ◽

Rubber Tree ◽

Mass Propagation ◽

Half Strength ◽

First Time

A protocol has been developed for induction, maturation and germination of the zygotic embryo derived callus of the rubber tree (Hevea brasiliensis Muell. Arg.). The influence of plant growth regulators (PGRs) including 2,4‐D, α‐NAA, picloram, GA3 and TDZ on MS and MMS were studied. Optimum calli were induced on MS supplemented with 2.0 mg/l 2, 4‐D. The best callus growth and proliferation was recorded on MS fortified with 2.0 mg/l 2, 4‐D + 2.0 mg/l BAP + 0.5 mg/l NAA. The maximum embryonic calli were induced on MS + 2.0 mg/l 2, 4‐D + 2.0 mg/l Kn medium. Embryo induction, differentiation and maturation were obtained on MMS (MS +Vit B5). The rooted plantlets were produced on half strength MS without any supplements.The novelty of this study is the induction of embryos and plant regeneration from zygotic embryo explants of Hevea for the first time. The protocol developed in this study will facilitate mass propagation of high yielding rubber clones as well as to develop transgenic rubber plants with desired genes through genetic transformation.Plant Tissue Cult. & Biotech. 27(1): 51-61, 2017 (June)

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