scholarly journals Perawatan Bonegraft dengan Penambahan Platelet-Rich Plasma dan Kolagen pada Kerusakan Infraboni

2016 ◽  
Vol 1 (1) ◽  
pp. 9
Author(s):  
Hendry Dwi Wijayanto ◽  
Kwartarini Murdiastuti
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Bone Allograft ◽  
Target Area ◽  
Bovine Bone ◽  
Radiographic Appearance ◽  
Freeze Dried ◽  
High Concentrations ◽  
Infrabony Defect

Perawatan kerusakan jaringan periodontal mempunyai tujuan utama mendapatkan jaringan regeneratif dan proses yang berlangsung membentuk struktur jaringan yang fungsional melalui proses pertumbuhan serta diferensiasi sel sel baru. Bonegraft adalah perawatan untuk kasus kerusakan tulang. Platelet-rich plasma yang merupakan platelet autologus konsentrasi tinggi tersuspensi dalam plasma setelah disentrifugasi. Dalam PRP banyak terdapat komponen yang berperan dalam proses penyembuhan regeneratif, growth faktor, agen kemotaktik dan agen vasoaktif. Kombinasi dengan penambahan kolagen merupakan altematif yang aman dan efektif, selain menstimulasi pelepasan growth faktor pada daerah target, juga memperkuat signal agar degranulasi platelet dapat ditingkatkan. Pada kasus ini, wanita berusia 24 tahun mengeluhkan keadaan giginya goyah sejak 3 bulan yang lalu karena traumatik, tidak ada rasa nyeri. Setelah dilakukan rontgen periapikal digital terlihat terjadinya kerusakan tulang infraboni. Penanganan untuk kasus ini dirancang dengan bedah flap, bonegraft Demineralized Freeze-Dried Bovine Bone Allograft (DFDBA), aplikasi platelet-rich plasma, serta penambahan kolagen. Kombinasi bonegraft dengan aplikasi PRP dan penambahan kolagen untuk menunjang perawatan periodontal memberikan hasil yang memuaskan secara penampakan klinis dan penampakan radiografis. ABSTRACT: Bonegraft Treatment with Addition of Platelet - Rich Plasma and Collagen in Infrabony Defect. The treatment of periodontal tissue damage has the main goal to get regenerative tissues and processes that take place to form a functional network structure through the process of cell growth and differentiation of new cells. Bonegraft is a treatment for cases of bone damage. Platelet-rich plasma is autologous platelets suspended in plasma high concentrations after centrifugation. In PRP, there are many components that play a role as a regenerative agent of healing process, growth factors, chemotactic agents and vasoactive agents. Its combination with the addition of collagen is a safe and effective alternative; in addition to stimulating the release of growth factors in the target area, it also strengthens the signal to improve platelet degranulation. In this case, a 24-year-old woman complained of unsteady state of her teeth since the last 3 months due to trauma; there was no pain. A digital periapical X-ray exposed infrabony defect. The treatment for this case was designed to use a surgical flap, bonegraft Demineralized Freeze-Dried Bovine Bone Allograft (DFDBA), application of platelet-rich plasma, as well as the addition of collagen. Bonegraft combination with PRP application and the addition of collagen to support periodontal treatment have given satisfactory results in the clinical and radiographic appearance.

scholarly journals ROLE OF PLATELET RICH PLASMA IN TREATMENT OF CHRONIC NON-HEALING VENOUS ULCER

2020 ◽  
Vol 4 (2) ◽  
Author(s):  
Firdaus A. Dekhaiya ◽  
Jignesh K. Joshi ◽  
Sarav Bamania
Keyword(s):  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Healing Process ◽  
Venous Ulcer ◽  
Lower Limbs ◽  
Venous Ulcers ◽  
Autologous Platelet Rich Plasma ◽  
Adhesive Proteins ◽  
Autologous Platelet

Introduction: Venous ulcers are wounds that are thought to occur due to improper functioning of venous valves, usually of the lower limbs. Considering that PRP is a source of growth factors, and consequently has mitogenic, angiogenic, and chemotactic properties, it represents an adjunctive treatment for recalcitrant wounds. Moreover, PRP provides the wound with adhesive proteins, such as fibrinogen, which are important in wound healing. PRP contain more amount of platelets, cytokines and growth factors which are dispersed in a very small amount of plasma which can be prepared from a sample of centrifuged autologous blood. Application of PRP has been reported to be effective in both acute as well as chronic non healing venous ulcers. Aim and Objective: To evaluate the efficacy of autologous platelet rich plasma in the management of chronic venous ulcer. Material and Method: A Prospective study conducted on 100 patients of chronic venous ulcers admitted in Sir T. Hospital Bhavnagar from June 2018 to June 2019 after fulfilled our inclusion and exclusion criteria. PRP then injected intalesionally inside and around the periphery of the wound/ulcer. This process was done once/week for 12 weeks. At every week, the area and volume of ulcer was calculated and photographs were taken. Result: All the patients showed healing of the ulcer with reduction in size of ulcer more than 90% was observed in 72 patients, followed by 80–90% reduction in wound size in 18 patients after the 12 weeks follow-up. Overall, significant reduction in size of ulcer was observed in all the treated patients. Conclusion: Autologous platelet rich plasma (PRP) as an autologous method, it is biocompatible, simple, safe, affordable and less expensive procedure in the treatment of chronic venous ulcers. PRP is found to be useful in improving and enhancing the healing process in chronic venous leg ulcers without any side effect. Keywords:  Venous ulcer, Platelet rich plasma

scholarly journals Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Livia Camargo Garbin ◽  
C. Wayne McIlwraith ◽  
David D. Frisbie
Keyword(s):  
Musculoskeletal Diseases ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Interleukin 1 ◽  
Platelet Lysate ◽  
Freeze Dried ◽  
Platelet Concentration ◽  
High Concentrations ◽  
Disease Modifying Treatment

Abstract Background Platelet-rich plasma (PRP) as well as other platelet-derived products have been used as a potential disease-modifying treatment for musculoskeletal diseases, such as osteoarthritis (OA). The restorative properties of such products rely mainly on the high concentrations of growth factors, demonstrating encouraging results experimentally and clinically. Yet, the autologous blood-derived nature of the PRP product lead to limitations that precludes it’s widespread use. The main limitations for PRP use are; product variability, the need for minimum laboratory settings in most cases, and the need for storage at low temperatures to preserve its properties. Based on these limitations, the objective of this study was to investigate an allogeneic off-the-shelf platelet lysate (PL) in cartilage exposed to interleukin 1β (IL-1β). For this purpose, blood and cartilage were harvested from eight skeletally mature and healthy horses. Blood was processed into PL aliquots and divided into three groups (Frozen, Freeze-dried and Filtered freeze-dried), used in autologous and allogeneic conditions and in three different concentrations (1.5, 3 and 6-fold). Different PL preparations were then applied in cartilage culture with interleukin-1 beta and cultured for 10 days. Cartilage and media samples were collected and analyzed for total GAG and 35SO4-labeled GAG content. Results No significant differences between the controls and PL groups in cartilage and media were demonstrated. The effects of PL on cartilage matrix were concentration dependent and intermediate concentrations (3-fold) in PL showed increased 35SO4-labelled GAG in cartilage. Conclusion In conclusion, the allogeneic freeze-dried PL presented equivalent effects compared to frozen autologous PL. Intermediate platelet concentration on average demonstrated improved results, demonstrating less GAG loss compared to other concentrations.

The Use of Platelet-Rich Plasma Combined With Demineralized Freeze-Dried Bone Allograft in the Treatment of Periodontal Endosseous Defects

2010 ◽  
Vol 141 (8) ◽  
pp. 967-978 ◽  
Author(s):  
Nikolaos Markou ◽  
Eudoxie Pepelassi ◽  
Sotirios Kotsovilis ◽  
Ioannis Vrotsos ◽  
Helen Vavouraki ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Bone Allograft ◽  
Freeze Dried

Treatment of Periodontal Endosseous Defects With Platelet-Rich Plasma Alone or in Combination With Demineralized Freeze-Dried Bone Allograft: A Comparative Clinical Trial

2009 ◽  
Vol 80 (12) ◽  
pp. 1911-1919 ◽  
Author(s):  
Nikolaos Markou ◽  
Eudoxie Pepelassi ◽  
Helen Vavouraki ◽  
Harry C. Stamatakis ◽  
Georgios Nikolopoulos ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Platelet Rich Plasma ◽  
Bone Allograft ◽  
Comparative Clinical Trial ◽  
Freeze Dried

Bone Formation in the Maxillary Sinus by Using Platelet-rich Plasma: An Experimental Study in Sheep

2005 ◽  
Vol 31 (1) ◽  
pp. 2-17 ◽  
Author(s):  
Edgar Grageda ◽  
Jaime L. Lozada ◽  
Phillip J. Boyne ◽  
Nicholas Caplanis ◽  
Paul J. McMillan
Keyword(s):  
Platelet Count ◽  
Bone Regeneration ◽  
Maxillary Sinus ◽  
Platelet Rich Plasma ◽  
Test Group ◽  
Bone Allograft ◽  
Autogenous Bone ◽  
Control Group ◽  
Freeze Dried ◽  
Log Ratio

Abstract Recently, platelet-rich plasma (PRP) has been proven to be an effective regeneration adjunct when combined with autogenous bone in the reconstruction of mandibular defects. However, little is known about the effect of PRP when combined with a bone allograft in the maxillary sinus. The purpose of this study was to quantitatively evaluate the ability of PRP to enhance bone regeneration in the maxillary sinus of sheep when combined with demineralized freeze-dried bone allograft (DFDBA) and cortical cancellous freeze-dried bone allograft (CCFDBA). Ten sheep were selected for bilateral sinus augmentation DFDBA + CCFDBA + PRP (test) and DFDBA + CCFDBA (control). Five were sacrificed at 3 months and the other 5 at 6 months. Hematology tests were performed for platelet count, and histology slides were obtained for histomorphometric analysis taking 2 measures of interest: total area (square millimeters) and percentage of bone fill. Student t tests showed no significant difference between test and control groups for total area (P > .25) and percentage of bone fill (P > .80) at either 3 or 6 months. The control group showed no statistical difference for total area (P < .095) and percentage of bone fill (P < .60) between 3- and 6-month healing times. The test group, however, showed a significant increase in total area (P < .025) but not in percentage of bone fill (P < .40) for the 2 healing periods. When the treatments were compared for interactions within the animal model, no clear tendency was evident for the test group to perform in relation to the control group regarding total area (r = .766, P < .01). A moderate tendency existed between the percentages of bone filled (r = .824, P < .005). Platelet-rich plasma showed higher platelet count than did the whole blood (2 to 5 times). However, no correlation was found between the log ratio and the bone measures. Within the limitations of this study, PRP failed to enhance or accelerate bone regeneration in the maxillary sinus of sheep when combined with bone allograft.

Treatment of Periodontal Intrabony Defects With Demineralized Freeze-Dried Bone Allograft in Combination With Platelet-Rich Plasma: A Comparative Clinical Trial

2008 ◽  
Vol 79 (5) ◽  
pp. 802-810 ◽  
Author(s):  
Matteo Piemontese ◽  
Simone Domenico Aspriello ◽  
Corrado Rubini ◽  
Luigi Ferrante ◽  
Maurizio Procaccini
Keyword(s):  
Clinical Trial ◽  
Platelet Rich Plasma ◽  
Bone Allograft ◽  
Intrabony Defects ◽  
Comparative Clinical Trial ◽  
Freeze Dried
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