ARTEMISININ CONTENT ON ARTEMISIA ANNUA L. TREATED BY GLORIOSA SUPERBA SEEDS’ WATER EXTRACT

Objective: The aim of this study was to determine the artemisinin content on Artemisia annua L. treated by water extract of Gloriosa superba seeds.Methods: G. superba seeds obtained naturally on Krakal Beach, Gunung Kidul, and extraction used a maceration method by water solvent (1:1). A. annua L. sprouts were obtained from B2P2TOOT Tawangmangu. Treatment variables done on sprouts using various water extract concentration of G. superba seeds and soaking time on A. annua L. sprouts. Determination of artemisinin content in leaf extract of A. annua L. was done using KLT-densitometric method with n-hexane:ethyl acetate (4:1) as mobile phase.Result: The result showed that artemisinin content in plant treatment of G. superba seed water extract was higher (9.78 μg/μl [±3.21]–16.60 μg/μl [±1.39]) compared to control plants (6.39 μg/μl [±1.40]). The concentration water extract of G. superba seed affected the level of artemisinin in the treatment plant. On the other hand, the soaking of A. annua L. sprouts using the water extract of G. superba seed did not affect the level of artemisinin content.Conclusion: Artemisinin content in treatment plant by G. superba seed water extract treatment was higher compared to control plants.

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Determination of Artemisinin Content in Artemisia annua L.

Mongolian Journal of Biological Sciences ◽

10.22353/mjbs.2011.09.06 ◽

2011 ◽

Vol 9 (1-2) ◽

Keyword(s):

Artemisia Annua ◽

Artemisia Annua L ◽

Artemisinin Content

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Size and Density of Artemisia annua Stomata Soaked in Water Extract of Gloriosa superba Seeds

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i3.11234 ◽

2017 ◽

Vol 9 (3) ◽

pp. 423

Author(s):

Sri Indah Rahmawati ◽

Ahmad Yunus ◽

Ari Susilowati

Keyword(s):

Artemisia Annua ◽

Stomatal Density ◽

Water Extract ◽

Herbaceous Plant ◽

Gloriosa Superba ◽

Stomatal Size ◽

Water Extracts ◽

Water Solvent ◽

Length Width ◽

Stomatal Length

Artemisia annua is a herbaceous plant that produces artemisinin as a malaria drug, haemorrhoids therapy, aromatherapy, antiviral, anticancer and antibacterial. Gloriosa superba is a plant that contains high colchicine compounds, especially on the seeds. Gloriosa superba extracts of tubers, stems, seeds, and leaves were used as biomutagen for many plants. Colchicine contains of these plants as antimitotic have been studied and proven by the mitotic index plants. Water extracts of Gloriosa superba seeds was used as a mutagen for Artemisia annua. The aim of this study was to determine the size and density of Artemisia annua stomata soaked in water extract of Gloriosa superba seeds as a mutagen. Extraction of Gloriosa superba seeds obtained naturally on Krakal Beach, Gunung Kidul by using a maceration method with water solvent (1:1). Artemisia annua sprouts were obtained from B2P2TOOT Tawangmangu. Variables treatment on sprouts using water extract concentration of Gloriosa superba seeds and soaking time of Artemisia annua sprouts. Measurements of stomatal length, width and density were conducted in epidermis of Artemisia annua leaf. Observation and measurements of the stomata were conducted by using a light microscope. The results showed that the length and width of stomata were 0.025 mm and 0.017 mm respectively. The stomatal density of the control leaf (174.69 amount/mm2) was lower than the other treated plants. Stomatal size and density has increased with the increasing concentration extracts on treated plants. Water extracts of Gloriosa superba seeds proved the effects on stomatal size and density of treated plants.

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