scholarly journals A SIMPLE AND EFFICIENT MICROPROPAGATION PROTOCOL FOR DEVELOPING PLANTLETS OF EXACUM BICOLOR ROXB. – AN ENDANGERED, ORNAMENTAL, AND ANTIDIABETIC HERB

2021 ◽  
pp. 36-40
Author(s):  
SHARAD S ACHAR ◽  
SAISHA VINJAMURI
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Simple Procedure ◽  
Vegetative Reproduction ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Apical Region ◽  
Indole Butyric Acid ◽  
Multiple Shoot Regeneration ◽  
Full Factorial

Objective: Exacum bicolor Roxb. is an endangered medicinal herb due to overexploitation by humans and its inefficient vegetative reproduction. Here, we report an efficient and simple procedure for the regeneration of E. bicolor Roxb. using leaf as an explant. Methods: The optimal concentrations of the hormones needed for callus induction were determined by full factorial method using DOE (Design expert ver. 8.0). The hormones selected based on literature were kinetin, indole acetic acid, and 6-Benzylaminopurine (BAP). Multiple shoot regeneration was carried out in liquid and solid media with the optimal concentrations of the hormones obtained by DOE. Rooting was initiated using Murashige and Skoog media containing naphthalene acetic acid 0.5 mg/l, indole butyric acid (IBA) 1.0 mg/l, and gibberellic acid 3 0.5 mg/l along with 0.2% of activated charcoal. Results: Analysis of full factorial design run showed that BAP in combination with kinetin was effective for the growth of callus and multiple shoot regeneration was higher in liquid media (81.25%). The rate of rooting was observed to be 88.23% and the average number of roots was 0.26. Plantlets with budding apical region and well-established leaves and roots were observed in 30 days. Conclusion: The protocol reported here can be used for effective production of E. bicolor plants in a shorter duration compared to the conventional approach.

scholarly journals In vitro propagation of Withania somnifera (L.) Dunal. from shoot apex explants

2014 ◽  
Vol 6 (1) ◽  
pp. 159-163 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Apex ◽  
Indole Acetic Acid ◽  
Withania Somnifera ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Hardening Process ◽  
Shoot Apex Explants

In vitro rapid micropropagation of Withania somnifera; has been achieved using shoot apex explants, when cultured on MS medium with different auxins: Indole Butyric Acid (IBA) 0.5-4 mg/l, Indole Acetic Acid (IAA) 2 mg/l and Naphthalene Acetic Acid (NAA) 0.5-3 mg/l and cytokinin: Benzyl Amino Purine (BAP) 0.5-4 mg/l. NAA and BAP stimulated direct as well as callus mediated multiple shoot. IBA alone and in combination with IAA stimulated rhizogenesis, while IBA with NAA proliferated callus-mediated rhizogenesis. The rooted plantlets through sequential hardening process could be established in the field.

scholarly journals Micropropagation of Salacia macrosperma Wight - An Endemic Medicinal Plant of Western Ghats

2020 ◽  
pp. 100-111
Author(s):  
C. Mahendra ◽  
M. S. Sudarshana
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Embryogenic Callus ◽  
Western Ghats ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Ms Medium ◽  
Multiple Shoot Regeneration ◽  
Developmental Features

Salacia macrosperma Wight. -  a potent medicinal plant facing the verge of rare and endemic status in the Western Ghats region of southern India. The effective protocol has been standardized for callus induction and multiple shoot regeneration using leaf and nodal explants. The Murashige and Skoog (MS) medium fortified with various plant growth regulators like 2,4-dichlorophenoxyacetic acid (2,4-D), Benzyl amino purine (BAP), Thidiazuron (TDZ), Indole acetic acid (IAA), Kinetin (Kn), Naphthalene acetic acid (NAA) and Indole butyric acid (IBA). The leaf explants produced more calli than nodal explants in MS medium supplemented with 2, 4-D and BAP in combination than individual hormones. Likewise, MS medium with 1.5 mg L-1of 2, 4-D, 2.5 mg L-1 of BAP and 1.5 mg L-1of TDZ along with 1% activated charcoal was apt for multiple shoot regeneration (93.33%) from nodal explants with slight embryogenic callus. Further, each developed plantlets were produced maximum rhizogenesis in liquid MS medium supplemented with 1.0 mg L-1 of IAA. Furthermore, the cytological study of embryogenic callus revealed variations in callus cells such as multinucleate, multi-nucleolate, cytodifferentiation, chromosomal bridges were noticed, besides normal dividing stages. Further, by scanning electron micrograph (SEM) analysis of embryogenic callus different stages of morphogenic developmental features were recorded.

TDz Induced Multiple Shoot Regeneration and in Vitro Flowering From Nodal Explant in Withania Somnifera (L.)

2012 ◽  
Vol 3 (4) ◽  
pp. 50-52
Author(s):  
Nishesh Sharma ◽  
◽  
Manjul Dhiman ◽  
Eapen P Koshy
Keyword(s):  
Shoot Regeneration ◽  
Withania Somnifera ◽  
Multiple Shoot ◽  
In Vitro Flowering ◽  
Nodal Explant ◽  
Multiple Shoot Regeneration

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals In vitro multiple shoot regeneration from hypocotyl in Menta spicata subsp. spicata

2015 ◽  
Vol 30 (2) ◽  
pp. 126
Author(s):  
Fethi Ahmet Özdemir ◽  
Mehmet Uğur Yıldırım ◽  
Mahsa Pourali Kahriz
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Multiple Shoot Regeneration

Multiple Shoot Regeneration Response of Recalcitrant Cotton (Gossypism hirsutum L.) Cultivar CIM-443

2004 ◽  
Vol 7 (8) ◽  
pp. 1371-1375 ◽  
Author(s):  
Rozina M. Ali ◽  
Tayyab Husnain . ◽  
Syed Sarfraz Hussain . ◽  
Nasir Mahmood . ◽  
Sheikh Riazuddin .
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Regeneration Response ◽  
Multiple Shoot Regeneration

Multiple Shoot Regeneration from Young Shoots of Kenaf (Hibiscus cannabinus)

2004 ◽  
Vol 77 (1) ◽  
pp. 49-53 ◽  
Author(s):  
Samanthi P. Herath ◽  
Takayuki Suzuki ◽  
Kazumi Hattori
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Hibiscus Cannabinus ◽  
Multiple Shoot Regeneration
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