scholarly journals Micropropagation of Salacia macrosperma Wight - An Endemic Medicinal Plant of Western Ghats

2020 ◽  
pp. 100-111
Author(s):  
C. Mahendra ◽  
M. S. Sudarshana
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Embryogenic Callus ◽  
Western Ghats ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Ms Medium ◽  
Multiple Shoot Regeneration ◽  
Developmental Features

Salacia macrosperma Wight. -  a potent medicinal plant facing the verge of rare and endemic status in the Western Ghats region of southern India. The effective protocol has been standardized for callus induction and multiple shoot regeneration using leaf and nodal explants. The Murashige and Skoog (MS) medium fortified with various plant growth regulators like 2,4-dichlorophenoxyacetic acid (2,4-D), Benzyl amino purine (BAP), Thidiazuron (TDZ), Indole acetic acid (IAA), Kinetin (Kn), Naphthalene acetic acid (NAA) and Indole butyric acid (IBA). The leaf explants produced more calli than nodal explants in MS medium supplemented with 2, 4-D and BAP in combination than individual hormones. Likewise, MS medium with 1.5 mg L-1of 2, 4-D, 2.5 mg L-1 of BAP and 1.5 mg L-1of TDZ along with 1% activated charcoal was apt for multiple shoot regeneration (93.33%) from nodal explants with slight embryogenic callus. Further, each developed plantlets were produced maximum rhizogenesis in liquid MS medium supplemented with 1.0 mg L-1 of IAA. Furthermore, the cytological study of embryogenic callus revealed variations in callus cells such as multinucleate, multi-nucleolate, cytodifferentiation, chromosomal bridges were noticed, besides normal dividing stages. Further, by scanning electron micrograph (SEM) analysis of embryogenic callus different stages of morphogenic developmental features were recorded.

scholarly journals An Improved Protocol for Multiple Shoot Regeneration from Seedlings and Mature Explants of Citrus macroptera Mont.

2009 ◽  
Vol 18 (1) ◽  
pp. 17-24
Author(s):  
Md. Nesawar Miah ◽  
Shahina Islam ◽  
Syed Hadiuzzaman
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Cow Dung ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Regeneration

Efforts have been made to establish a protocol for direct multiple shoot regeneration from both in vitro grown seedlings and mature plants of Citrus macroptera. Both nodal and shoot tip explants taken from in vitro grown seedlings were cultured in MS supplemented with different concentrations of BAP and Kn either singly or in combinations. Both these explants are capable to regenerate and produce in vitro multiple shoots. Maximum number of shoots were obtained from nodal explants in MS supplemented with 1.0 mg/l BAP. BAP alone was found superior to Kn. On the other hand, only nodal explants from mature plants were used and 1.0 mg/1 BAP was also found best suitable for shoot induction and multiplication. Ex vitro rooting in pot soil (mixed with biogas slurry derived from cow-dung) was most successful compared to in vitro rooting in half strength of MS supplemented with different concentrations of NAA and IBA. Key words: In vitro, Shoot regeneration, Citrus macroptera D.O.I. 10.3329/ptcb.v18i1.3246 Plant Tissue Cult. & Biotech. 18(1): 17-24, 2008 (June)

scholarly journals An Efficient Protocol for High-frequency Direct Multiple Shoot Regeneration from Internodes of Peppermint (Mentha x piperita)

2010 ◽  
Vol 5 (12) ◽  
pp. 1934578X1000501
Author(s):  
Sanjog T. Thul ◽  
Arun K. Kukreja
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Mentha X Piperita ◽  
Half Strength ◽  
Multiple Shoot Regeneration

A simple, repeatable and efficient protocol for direct multiple shoot regeneration from internodal explants has been defined in peppermint ( Mentha x piperita var. Indus). In vitro regenerated shoots of peppermint were excised into 4 to 8 mm long internodes and cultured on Murashige and Skoog's medium supplemented with different cytokinins. In the hormonal assay, 3.0 mg L-l zeatin or 6-isopentenyl adenine independently supplemented to half strength MS medium exhibited multiple shoot regeneration, while thiaduzorn (0.1-3.0 mg L−1) showed no morphogenetic effect. A maximum of 85% in vitro cultured explants showed multiple shoot formation with an average of 7 shoots per explant on MS medium supplemented with zeatin. Multiple shoots were initiated within three weeks of cultivation. Internodes with regenerated multiple shoots were transferred to half - strength MS medium without supplementing with any plant growth hormone for shoot elongation and rhizogenesis. Rooted plants acclimatized and grew to maturity under glasshouse conditions. The plantlets developed were phenotypically identical to the parent plant and exhibited 96 % survival.

scholarly journals Conservation of the endangered medicinal plant Picrorhiza kurroa through in vitro multiple shoot regeneration

2019 ◽  
Vol 100 (3) ◽  
pp. 209-217
Author(s):  
Varnika Chaudhary ◽  
Shalini Singh ◽  
Rohit Sharma ◽  
Ajay Singh ◽  
Nishesh Sharma
Keyword(s):  
Medicinal Plant ◽  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Picrorhiza Kurroa ◽  
Endangered Medicinal Plant ◽  
Multiple Shoot Regeneration

scholarly journals In vitro Micropropagation of Bacopa monnieri (L.) Penn. - An Important Medicinal Plant

2020 ◽  
Vol 30 (1) ◽  
pp. 57-63
Author(s):  
Md Adnan Rahe ◽  
Sanjida Rahman Mollika ◽  
M Salim Khan ◽  
Tanjina Akhtar Banu ◽  
GM Al Amin ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Bacopa Monnieri ◽  
Nodal Explants ◽  
Best Response ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Important Medicinal Plant

Investigation on in vitro multiple shoot regeneration in Bacopa monnieri (L.) Penn. using leaf and nodal explants was carried out on MS containing various concentrations and combinations of BAP, Kn, NAA and 2,4-D. Of the two explants, leaf showed the best response towards shoot regeneration and subsequent plant development on MS with 1.0 mg/l BAP and 0.25 mg/l Kn. In this combination, the mean number of shoots/explant was 10.6 ± 0.11 in leaf and 9.6 ± 0.29 in nodal explants. Maximum shoot length was recorded as 12.6 ± 0.21 and 11.20 ± 0.30 from leaf and nodal explants after six weeks of culture, respectively. Half strength of MS supplemented with 0.25 mg/l IBA was found to be the best medium for root formation. The in vitro regenerated plantlets were successfully transplanted in soil after acclimatization. Plant Tissue Cult. & Biotech. 30(1): 57-63, 2020 (June)

scholarly journals In vitro multiple shoot regeneration from corm bud explant in ghet kachu, typhonium trilobatum schott - a medicinal aroid

2012 ◽  
Vol 47 (2) ◽  
pp. 211-216
Author(s):  
KK Paul ◽  
MA Bari
Keyword(s):  
Shoot Regeneration ◽  
White Light ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Multiple Shoot Regeneration ◽  
Regenerated Plantlets ◽  
Light Green

An efficient in vitro regeneration protocol was developed in medicinal aroid, Ghetkachu (Typhonium trilobatum Schott) using field grown corm bud explant. Highest percentage (75 %) of direct multiple shoot regeneration obtained in MS media supplemented with 5.0 mgL-1BAP + 1.5mg L-1NAA. Callus formation occur (80 %) in MS media containing 0.5mgL-1BAP + 2.0mgL-1NAA. The appearance of calli was white, creamy white light green in colour and the texture of calli were soft, friable and semi hard and compact. Shoot regeneration (85 %) obtained from calli in MS medium having 5.0mgL-1BAP +1.0mgL-1NAA. The regenerated plantlets were successfully acclimatized with loamy fertile soil and survived cent percentage in natural condition.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11454   Bangladesh J. Sci. Ind. Res. 47(2), 211-216, 2012  

scholarly journals In vitro Regeneration in Three Varieties of White jute (Corchorus capsularis L.)

1970 ◽  
Vol 17 (1) ◽  
pp. 11-18 ◽  
Author(s):  
RH Sarker ◽  
GM Al-Amin ◽  
MI Hoque
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
The Other ◽  
Nodal Explants ◽  
Root Induction ◽  
Corchorus Capsularis ◽  
Best Response ◽  
Multiple Shoot Regeneration

Healthy multiple shoot regeneration was observed from petiole-attached cotyledon (PC) explants of CVL-1 on MS containing 0.2 mg/l BAP and 1.0 mg/l IAA. On the other hand, the best response for multiple shoot regeneration in CVE-3 was obtained when the same explants were cultured on MS with 2.5 mg/l BAP and 0.5 mg/l NAA. However, the same explant of D-154 was found to show less responsive compared to other two varieties to produce multiple shoots. Cotyledonary nodal explants of all the three varieties were found to produce maximum number of multiple shoots on MS supplemented with 0.2 mg/l BAP and 1.0 mg/l IAA. Best root induction was observed at the base of the in vitro regenerated shoots on half the strength of MS supplemented with 0.3 mg/l IBA. The in vitro grown plantlets were successfully transplanted into soil. They grew up to maturity, flowered and fruited like the control plants.Key words: Regeneration, White jute, Corchorus capsularisDOI = 10.3329/ptcb.v17i1.1116Plant Tissue Cult. & Biotech. 17(1): 11-18, 2007 (June)

scholarly journals Multiple shoot induction and regeneration of Vanilla borneensis Rolfe - a critically endangered orchid of Assam, India

2021 ◽  
Author(s):  
Sneha Hasnu ◽  
Bhaben Tanti
Keyword(s):  
Shoot Regeneration ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Critically Endangered ◽  
Root Initiation ◽  
Shoot Induction ◽  
Endangered Orchid ◽  
Bud Induction ◽  
Multiple Shoot Regeneration

In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – a critically endangered orchid through multiple shoot regeneration. Through in vitro multiple shoot regeneration from both nodal and shoot tip explants, maximum (100%) shoot induction was observed. The minimum time required for shoot bud induction was observed from the shoot tip (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 mM) as compared to the nodal explants. Maximum multiple shoot regeneration was observed from nodal explants on the medium supplemented with BAP (4.44 mM) + TDZ (6.82 mM). However, maximum shoot length was observed on the medium supplemented with BAP (4.44 mM) + 15% CW and the number of nodes (5.27±0.33) per shoot after 90 days. Maximum (80-100%) of root initiation was observed in almost all the concentrations of NAA. The shortest time of root initiation was found on the medium supplemented with NAA (5.37 mM). Further, acclimatization period was found to be 15 days with 70% acclimatization while 60% of survivability was observed in the field condition. This efficient micropropagation method of V. borneensis could be successfully used for mass propagation as well as conservation of the critically endangered wild orchid.

scholarly journals A SIMPLE AND EFFICIENT MICROPROPAGATION PROTOCOL FOR DEVELOPING PLANTLETS OF EXACUM BICOLOR ROXB. – AN ENDANGERED, ORNAMENTAL, AND ANTIDIABETIC HERB

2021 ◽  
pp. 36-40
Author(s):  
SHARAD S ACHAR ◽  
SAISHA VINJAMURI
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Simple Procedure ◽  
Vegetative Reproduction ◽  
Multiple Shoot ◽  
Naphthalene Acetic Acid ◽  
Apical Region ◽  
Indole Butyric Acid ◽  
Multiple Shoot Regeneration ◽  
Full Factorial

Objective: Exacum bicolor Roxb. is an endangered medicinal herb due to overexploitation by humans and its inefficient vegetative reproduction. Here, we report an efficient and simple procedure for the regeneration of E. bicolor Roxb. using leaf as an explant. Methods: The optimal concentrations of the hormones needed for callus induction were determined by full factorial method using DOE (Design expert ver. 8.0). The hormones selected based on literature were kinetin, indole acetic acid, and 6-Benzylaminopurine (BAP). Multiple shoot regeneration was carried out in liquid and solid media with the optimal concentrations of the hormones obtained by DOE. Rooting was initiated using Murashige and Skoog media containing naphthalene acetic acid 0.5 mg/l, indole butyric acid (IBA) 1.0 mg/l, and gibberellic acid 3 0.5 mg/l along with 0.2% of activated charcoal. Results: Analysis of full factorial design run showed that BAP in combination with kinetin was effective for the growth of callus and multiple shoot regeneration was higher in liquid media (81.25%). The rate of rooting was observed to be 88.23% and the average number of roots was 0.26. Plantlets with budding apical region and well-established leaves and roots were observed in 30 days. Conclusion: The protocol reported here can be used for effective production of E. bicolor plants in a shorter duration compared to the conventional approach.

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