scholarly journals OPTIMIZATION OF SODIUM DEOXYCHOLATE-BASED TRANSFERSOMES FOR PERCUTANEOUS DELIVERY OF PEPTIDES AND PROTEINS

2019 ◽  
pp. 329-332 ◽  
Author(s):  
ASTRIED LEONYZA ◽  
SILVIA SURINI
Keyword(s):  
Thin Film ◽  
Particle Size ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Zeta Potential ◽  
Sodium Deoxycholate ◽  
Polydispersity Index ◽  
Tem Analysis ◽  
Epidermal Growth ◽  
Preliminary Study

Objective: This study aims to formulate and characterize the transfersomes for percutaneous delivery of peptides and proteins. In particular, this study was a preliminary study for the transfersomes formulation of recombinant human epidermal growth factor (rhEGF) for topical delivery. Methods: The transfersomes was prepared by thin film hydration method using phosphatidylcholine and sodium deoxycholate as vesicle former. In this study, transfersomes formulas were optimized, namely TF1, TF2, TF3, and TF4 with several ratios of phospholipid and surfactant which were 90:10, 85:15, 80:20, and 75:25, respectively. Afterward, the transfersomes were characterized in terms of particle size distribution, polydispersity index, zeta potential, morphology of vesicles, and deformability index. Results: The results showed that the best formulation was TF3 with the ratio of 80:20 with a particle size of 118.6±1.33 nm, polydispersity index of 0.102±0.011, zeta potential of-30.9±0,46 mV, and deformability index of 1.182±0.08. TEM analysis also showed spherical and unilamellar vesicles of transfersomes. Conclusion: This work demonstrated that the sodium deoxycholate-based transfersomes could be potential to be further formulated with peptide and protein for percutaneous delivery.

Activation of epidermal growth factor receptor kinase in gastric carcinoma: a preliminary study

2000 ◽  
Vol 95 (8) ◽  
pp. 2135-2136 ◽  
Author(s):  
Tsutomu Masaki ◽  
Yoshio Hatanaka ◽  
Mikio Nishioka ◽  
Masaki Tokuda ◽  
Yasushi Shiratori ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Gastric Carcinoma ◽  
Growth Factor Receptor ◽  
Receptor Kinase ◽  
Epidermal Growth ◽  
Preliminary Study

Epidermal growth factor receptor expression in stage II-III colon carcinoma: A preliminary study

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 9718-9718
Author(s):  
C. Pericay ◽  
E. Musulén ◽  
Y. García-García ◽  
S. Fernández ◽  
L. Fernández-Morales ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Colon Carcinoma ◽  
Growth Factor Receptor ◽  
Receptor Expression ◽  
Stage Ii ◽  
Epidermal Growth ◽  
Preliminary Study

Epidermal growth factor receptor expression in stage II-III colon carcinoma: A preliminary study

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 9718-9718
Author(s):  
C. Pericay ◽  
E. Musulén ◽  
Y. García-García ◽  
S. Fernández ◽  
L. Fernández-Morales ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Colon Carcinoma ◽  
Growth Factor Receptor ◽  
Receptor Expression ◽  
Stage Ii ◽  
Epidermal Growth ◽  
Preliminary Study

scholarly journals Is salivary epidermal growth factor a biomarker for oral leukoplakia? A preliminary study

2015 ◽  
Vol 119 (4) ◽  
pp. 451-458 ◽  
Author(s):  
Filipe Jaeger ◽  
Ana Carla Assunção ◽  
Patrícia Carlos Caldeira ◽  
Celso Martins Queiroz-Junior ◽  
Vanessa Fátima Bernardes ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Oral Leukoplakia ◽  
Epidermal Growth ◽  
Preliminary Study

Oestrogen Induces C-Ha-Ras Expression in the Fibroblasts Derived from Human Uterine Endometrium

1995 ◽  
Vol 32 (5) ◽  
pp. 487-492 ◽  
Author(s):  
Jiro Fujimoto ◽  
Satoshi Ichigo ◽  
Masashi Hori ◽  
Shigeo Morishita ◽  
Teruhiko Tamaya
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Effective Concentration ◽  
Endometrial Stroma ◽  
Uterine Endometrium ◽  
Rapid Induction ◽  
Epidermal Growth ◽  
Preliminary Study ◽  
Dependent Growth ◽  
Human Uterine Endometrium

In this preliminary study, fibroblasts derived from uterine endometrium as a substitute for normal endometrial stroma were used to analyse the stromal role in uterine endometrium, which depends on oestrogen for growth. C-Ha- ras expression and tyrosine kinase (TK) activity in the fibroblasts were increased by oestradiol, and the increase was diminished by progesterone. Epidermal growth factor (EGF), an activator of TK, also increased c-Ha- ras expression. The combination of both oestradiol and EGF at maximum effective concentration exerted no additive and synergistic effect on induction of c-Ha- ras expression. Pretreatment with tyrphostin, an inhibitor of TK, abolished the oestrogen-inducible expression of c-Ha- ras. Oestrogen might lead to rapid induction of c-Ha- ras expression in endometrial stroma, at least in part, via the activation of TK for the early stages of oestrogen dependent growth in endometrium.

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