scholarly journals STANDARDIZATION AND APPLICATION OF PCR TARGETING CHLORELLA SPECIES ISOLATED FROM ENVIRONMENTAL SAMPLES

2021 ◽  
pp. 28-31
Author(s):  
PREMINA S. ◽  
NIREN ANDREW S. ◽  
SUNDARALINGAM R. ◽  
N. MURUGAN ◽  
SHARANYA THERESA V.
Keyword(s):  
Ribosomal Rna ◽  
Algal Species ◽  
Chlorella Sorokiniana ◽  
Species Level ◽  
Its1 Region ◽  
18S Ribosomal Rna ◽  
Search Tool ◽  
Chlorella Species ◽  
Pcr Targeting ◽  
Pcr Conditions

Objective: Identification of Chlorella species from the environment through 18s ribosomal RNA sequencing. This study was aimed to design primer targeting Chlorella and other closely related algal species targeting 18s ribosomal RNA, ITS1 region. Methods: Sanger sequencing was carried out for the identification of algae up to the genus and species level using an in-house designed primer and optimized PCR conditions. Results: Out of 2 algae samples identified phenotypically, one isolate identified as Chlorella vulgaris and other one identified as Chlorella sorokiniana based on the results of Basic Alignment Search Tool (BLAST). Conclusion: To conclude, this study provided primers with PCR conditions to characterize algal samples through molecular identification with 100% accuracy than the phenotypic method.

scholarly journals Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1149
Author(s):  
Dina M. Metwally ◽  
Isra M. Al-Turaiki ◽  
Najwa Altwaijry ◽  
Samia Q. Alghamdi ◽  
Abdullah D. Alanazi
Keyword(s):  
Saudi Arabia ◽  
Infection Rate ◽  
Ribosomal Rna ◽  
Phylogenetic Analyses ◽  
Trypanosoma Evansi ◽  
Camelus Dromedarius ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene ◽  
Polymerase Chain ◽  
Pcr Targeting

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

scholarly journals Corrigendum to: PEMA: a flexible pipeline for environmental DNA metabarcoding analysis of the 16S/18S ribosomal RNA, ITS, and COI marker genes

GigaScience ◽  
2020 ◽  
Vol 9 (12) ◽  
Author(s):  
Haris Zafeiropoulos ◽  
Ha Quoc Viet ◽  
Katerina Vasileiadou ◽  
Antonis Potirakis ◽  
Christos Arvanitidis ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Environmental Dna ◽  
Marker Genes ◽  
18S Ribosomal Rna ◽  
Dna Metabarcoding

scholarly journals The 18S ribosomal RNA m 6 A methyltransferase Mettl5 is required for normal walking behavior in Drosophila

EMBO Reports ◽  
2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Jessica Leismann ◽  
Mariangela Spagnuolo ◽  
Mihika Pradhan ◽  
Ludivine Wacheul ◽  
Minh Anh Vu ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
Normal Walking ◽  
Walking Behavior ◽  
18S Ribosomal Rna

Systematics of Holometabolous Insect Orders Based on 18S Ribosomal RNA

1993 ◽  
Vol 2 (2) ◽  
pp. 132-142 ◽  
Author(s):  
Dorothy P. Pashley ◽  
Bruce A. McPheron ◽  
Elizabeth A. Zimmer
Keyword(s):  
Ribosomal Rna ◽  
18S Ribosomal Rna ◽  
Holometabolous Insect

Chemical probing of adenine residues within the secondary structure of rabbit 18S ribosomal RNA

Biochemistry ◽  
1988 ◽  
Vol 27 (2) ◽  
pp. 582-592 ◽  
Author(s):  
Asha Rairkar ◽  
Heidi M. Rubino ◽  
Raymond E. Lockard
Keyword(s):  
Secondary Structure ◽  
Ribosomal Rna ◽  
18S Ribosomal Rna ◽  
Chemical Probing

scholarly journals Phylogenetic analysis based on 18S ribosomal RNA gene sequences supports the existence of class polyacanthocephala (acanthocephala)

2002 ◽  
Vol 23 (2) ◽  
pp. 288-292 ◽  
Author(s):  
Martı́n Garcı́a-Varela ◽  
Michael P Cummings ◽  
Gerardo Pérez-Ponce de León ◽  
Scott L Gardner ◽  
Juan P Laclette
Keyword(s):  
Phylogenetic Analysis ◽  
Ribosomal Rna ◽  
Gene Sequences ◽  
Ribosomal Rna Gene ◽  
18S Ribosomal Rna ◽  
18S Ribosomal Rna Gene
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