Molecular Identification of Trypanosoma evansi Isolated from Arabian Camels (Camelus dromedarius) in Riyadh and Al-Qassim, Saudi Arabia

We analyzed the blood from 400 one-humped camels, Camelus dromedarius (C. dromedarius), in Riyadh and Al-Qassim, Saudi Arabia to determine if they were infected with the parasite Trypanosoma spp. Polymerase chain reaction (PCR) targeting the internal transcribed spacer 1 (ITS1) gene was used to detect the prevalence of Trypanosoma spp. in the camels. Trypanosoma evansi (T. evansi) was detected in 79 of 200 camels in Riyadh, an infection rate of 39.5%, and in 92 of 200 camels in Al-Qassim, an infection rate of 46%. Sequence and phylogenetic analyses revealed that the isolated T. evansi was closely related to the T. evansi that was detected in C. dromedarius in Egypt and the T. evansi strain B15.1 18S ribosomal RNA gene identified from buffalo in Thailand. A BLAST search revealed that the sequences are also similar to those of T. evansi from beef cattle in Thailand and to T. brucei B8/18 18S ribosomal RNA from pigs in Nigeria.

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Molecular typing of phlebotomine sand flies in al-madinah and asir regions, Saudi Arabia using PCR–RFLP of 18S ribosomal RNA gene

Saudi Journal of Biological Sciences ◽

10.1016/j.sjbs.2016.01.014 ◽

2017 ◽

Vol 24 (7) ◽

pp. 1697-1703 ◽

Cited By ~ 4

Author(s):

Abeer A. Al-Dakhil ◽

Reem A. Al-Ajmi ◽

Nikhat J. Siddiqi ◽

Tahany H. Ayaad

Keyword(s):

Saudi Arabia ◽

Ribosomal Rna ◽

Molecular Typing ◽

Sand Flies ◽

Phlebotomine Sand Flies ◽

Ribosomal Rna Gene ◽

18S Ribosomal Rna ◽

18S Ribosomal Rna Gene ◽

Pcr Rflp

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Simultaneous quantification of 18S Ribosomal RNA gene and Mitochondria DNA: A non-radioactive polymerase chain reaction (PCR) protocol

Protein Engineering Design and Selection ◽

10.1093/protein/6.supplement.55 ◽

1993 ◽

Keyword(s):

Polymerase Chain Reaction ◽

Ribosomal Rna ◽

Chain Reaction ◽

Ribosomal Rna Gene ◽

18S Ribosomal Rna ◽

Simultaneous Quantification ◽

18S Ribosomal Rna Gene ◽

Mitochondria Dna ◽

Polymerase Chain

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Detection ofCryptosporidiumspecies in feces or gastric contents from snakes and lizards as determined by polymerase chain reaction analysis and partial sequencing of the 18S ribosomal RNA gene

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638711403415 ◽

2011 ◽

Vol 23 (3) ◽

pp. 430-435 ◽

Cited By ~ 23

Author(s):

Barbara Richter ◽

Nora Nedorost ◽

Anton Maderner ◽

Herbert Weissenböck

Keyword(s):

Polymerase Chain Reaction ◽

Ribosomal Rna ◽

Polymerase Chain Reaction Analysis ◽

Chain Reaction ◽

Partial Sequencing ◽

Ribosomal Rna Gene ◽

18S Ribosomal Rna ◽

18S Ribosomal Rna Gene ◽

Gastric Contents ◽

Polymerase Chain

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Molecular detection and identification of Babesia bovis and Trypanosoma spp. in one-humped camel (Camelus dromedarius) breeds in Egypt

Veterinary World ◽

10.14202/vetworld.2021.625-633 ◽

2021 ◽

Vol 14 (3) ◽

pp. 625-633

Author(s):

Shimaa Abd El-Salam El-Sayed ◽

Mohamed A. El-Adl ◽

Mayar O. Ali ◽

Mostafa Al-Araby ◽

Mosaab A. Omar ◽

...

Keyword(s):

Infection Rate ◽

Phylogenetic Analyses ◽

The United States ◽

Trypanosoma Evansi ◽

Babesia Bovis ◽

Camelus Dromedarius ◽

Effective Prevention ◽

Economic Significance ◽

Detection And Identification ◽

Unique Source

Background and Aim: Camels are a unique source of milk and meat, which helps recover from several diseases that affect humans worldwide. In Egypt, one of the great obstacles for this industry is tick-borne diseases. This study aimed to characterize blood parasite infections, such as Babesia (B.) bovis and Trypanosoma (T.) spp. in one-humped camel (Camelus dromedarius) (n=142) breeds in Halayeb and Shalateen, Egypt, through phylogenetic analysis. Materials and Methods: The prevalence of B. bovis and Trypanosoma spp. was identified in camels using polymerase chain reaction (PCR) assays targeting the Rhoptry-Associated Protein-1 and internal transcribed spacer 1 genes, respectively. A nested PCR technique was conducted to detect B. bovis. At the same time, KIN multispecies PCR assay was employed to diagnose and classify trypanosome DNA in camels. Results: B. bovis was detected in 4/142 camels with an infection rate of 2.81%. Sequencing and phylogenetic analyses revealed that the strain of B. bovis isolated from this population was closely related to strains isolated from Argentine, the United States, and Brazil. Moreover, Trypanosoma evansi was detected in 8/142 camels with an infection rate of 5.63%. Sequencing and phylogenetic analyses revealed that this isolated strain T. evansi was closely related to Trypanosoma theileri detected from cattle in Brazil. Conclusion: The obtained data indicated the existence of B. bovis and T. evansi in camels from two provinces of Egypt. The obtained findings have economic significance and reflect the importance of implementing effective prevention and control methods across Egypt to reduce the incidence of B. bovis and T. evansi in camels.

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