scholarly journals Molecular Diagnosis of Sexually Transmitted Infections (STI) in Symptomatic Women of Puducherry by a Commercial Real Time Multiplex PCR, FTD Urethritis Plus - A Preliminary Report

2019 ◽  
Vol 13 (3) ◽  
pp. 1791-1796
Author(s):  
Selvaraj Stephen ◽  
Jothimani Pradeep ◽  
Chinnadurai Rajkumar ◽  
Rupal Samal ◽  
Ashwini Vishalakshi ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Sexually Transmitted Infections ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Preliminary Report ◽  
Sexually Transmitted

scholarly journals Sexually Transmitted Infections Detected by Multiplex Real Time PCR in Asymptomatic Women and Association with Cervical Intraepithelial Neoplasia

2018 ◽  
Vol 40 (09) ◽  
pp. 540-546 ◽  
Author(s):  
Luiza Lima ◽  
Carolina Hoelzle ◽  
Renata Simões ◽  
Maria Lima ◽  
Jordana Fradico ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Cervical Intraepithelial Neoplasia ◽  
Sexually Transmitted Infections ◽  
Real Time ◽  
Ureaplasma Urealyticum ◽  
Significant Risk ◽  
Intraepithelial Neoplasia ◽  
Sexually Transmitted ◽  
Asymptomatic Patients ◽  
Asymptomatic Women

Objective To determine the frequency of sexually transmitted infections (STIs) in asymptomatic women and the association of STIs with cervical intraepithelial neoplasia (CIN). Methods A cross-sectional study was performed, enrolling women examined in a general gynecology clinic and in a colposcopy referral center from October 2014 to October 2015. The colposcopy group consisted of 71 women, and the general gynecology group consisted of 55 women. Cervical samples were collected for cervical cytology and a multiplex real-time polymerase chain reaction (PCR) was developed to detect human papillomavirus (HPV) and the STIs caused by the following microorganisms: Chlamydia trachomatis, Mycoplasma hominis, Mycoplasma genitalium, Ureaplasma urealyticum, and Neisseria gonorrhoeae. A multivariate analysis was performed by logistic regression, considering the significance level of 0.05. Results The general frequency of STIs was: 46.8% (HPV); 27.8% (C. trachomatis); 28.6% (M. genitalium); 0.8% (M. hominis); 4.8% (U. urealyticum); and 4.8% (N. gonorrhoeae). The significant risk factors for CIN were: HPV infection (odds ratio [OR] = 2.53; p = 0.024); C. trachomatis (OR = 3.04; p = 0.009); M. genitalium (OR = 2.37; p = 0.04); and HPV and C. trachomatis coinfection (OR = 3.11; p = 0.023). After the multivariate analysis, a significant association was found between HPV and CIN (OR = 2.48; 95% confidence interval [95%CI]: 1.04–5.92; p = 0.04); and between C. trachomatis and CIN (OR = 2.69; 95%CI: 1.11–6.53; p = 0.028). Conclusion The frequency of STIs was high in asymptomatic patients. Infections by HPV and C. trachomatis were independently associated with the presence of CIN. The high frequency of STIs in asymptomatic women suggests the need for routine screening of these infections.

scholarly journals A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections

2013 ◽  
Vol 7 (1) ◽  
pp. 68-74 ◽  
Author(s):  
Gyeong-In Lee ◽  
Jong Pil Yoen ◽  
Jin Seok Kang ◽  
Seung Yong Hwang ◽  
Yu-Min Hong ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Real Time ◽  
Real Time Pcr ◽  
Sexually Transmitted

Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections

2021 ◽  
Vol 39 (5) ◽  
pp. 229-233
Author(s):  
Cristina Casañ López ◽  
Belén Rivaya Sánchez ◽  
Gema Fernández Rivas ◽  
Águeda Hernández Rodríguez ◽  
Adrián Antuori Torres ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Sexually Transmitted Diseases ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Sexually Transmitted

Evaluation of VIASURE Sexually Transmitted Diseases Real Time PCR Detection kit (CerTest Biotec) for the diagnostic of sexually transmitted infections

2020 ◽  
Author(s):  
Cristina Casañ López ◽  
Belén Rivaya Sánchez ◽  
Gema Fernández Rivas ◽  
Águeda Hernández Rodríguez ◽  
Adrián Antouri Torres ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Sexually Transmitted Diseases ◽  
Real Time ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Sexually Transmitted

scholarly journals Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods

2013 ◽  
Vol 17 (12) ◽  
pp. e1134-e1140 ◽  
Author(s):  
Hyun-Sop Choe ◽  
Dong Sup Lee ◽  
Seung-Ju Lee ◽  
Sung-Hoo Hong ◽  
Dong Choon Park ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Real Time ◽  
Real Time Pcr ◽  
Sexually Transmitted

Multiplex PCR testing for nine different sexually transmitted infections

2016 ◽  
Vol 27 (14) ◽  
pp. 1275-1282 ◽  
Author(s):  
John D Kriesel ◽  
Amiteshwar S Bhatia ◽  
Cammie Barrus ◽  
Mike Vaughn ◽  
Jordan Gardner ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Sexually Transmitted Infections ◽  
Sexually Transmitted
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