scholarly journals Molecular Identification, Production and Optimization of Lipase from Oil Contaminated Soil using Submerged Fermentation

2020 ◽  
Vol 14 (1) ◽  
pp. 341-349
Author(s):  
P Joyruth ◽  
◽  
Lali Growther ◽  
Keyword(s):  
Molecular Identification ◽  
Contaminated Soil ◽  
Submerged Fermentation

scholarly journals The Application of Manure (Poultry Wests) and Bio-Formulations of Trichoderma harzianum and T. viride and Their Interaction to Control Root-knot Disease on Radish and Chard

2019 ◽  
Vol 32 (1) ◽  
pp. 88-98
Author(s):  
Nuha A. Al-Zehebawi ◽  
Dhia S. Al-Waily ◽  
Labeed A. Al-Saad
Keyword(s):  
Plant Height ◽  
Molecular Identification ◽  
Contaminated Soil ◽  
Trichoderma Harzianum ◽  
Control Treatment ◽  
Root Knot Nematode ◽  
Green House ◽  
Control Root ◽  
Positive Treatment

The study was designed to examine the effect of manure (poultry wastes) and bio-formulations of Trichoderma harzianum and T. viride separately or with some, to control root-knot disease on radish (Raphanus sativus L.) and chard (Beta vulagaris var.cicla (L.)). The study included the isolation of pathogenic nematode of both plant roots, morphological and molecular identification, examination of the pathogenicity in vitro and in vivo and green house experiments involved application of manure and fungal bio-formulations treatments. The morphological and molecular identification confirmed the identity of root-knot nematode, as Meloidogyne javanica, which was pathogenic to radish and chard. The recorded infection severity was 89 and 95% respectively. The green house experiment results revealed that MThTv treatment was significantly reduced infection severity to 0% for radish and chard in contaminated soil (CS) compared with control positive treatment (55.17 and 40%) respectively. MThTv treatment also showed a highest plant height for Radish in non-nematode-contaminated soil (NCS) and CS treatment (17.85 and 16.50 cm) respectively compared with control positive treatment (5.00 cm), while the highest plant height of Chard was 24.5 cm in MThTv-NCS. The wet weight index in Radish showed a superiority of MThTv and MTh in NC on other treatments (201.75 and 189.5 g.plant-1) respectively followed by MThTv-NCS treatment (184.5 g.plant-1) compared with 19.25 gm.plant-1 in control treatment. In Chard the results showed similar pattern represented by superiority of MThTv-NC treatment (255.25 gm.plant-1) followed by MThTv-NCS (190.75 gm.plant-1) compared with 37.50 gm/plant for positive control.

scholarly journals Production and Characterization of Lipases by Two New Isolates ofAspergillusthrough Solid-State and Submerged Fermentation

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Luciane Maria Colla ◽  
Aline M. M. Ficanha ◽  
Juliana Rizzardi ◽  
Telma Elita Bertolin ◽  
Christian Oliveira Reinehr ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Contaminated Soil ◽  
Submerged Fermentation ◽  
Residual Activity ◽  
Acidic Ph ◽  
Alkaline Ph ◽  
Fungal Strains ◽  
Different Sources

Due to the numerous applications of lipases in industry, there is a need to study their characteristics, because lipases obtained from different sources may present different properties. The aim of this work was to accomplish the partial characterization of lipases obtained through submerged fermentation and solid-state fermentation by two species ofAspergillus. Fungal strains were isolated from a diesel-contaminated soil and selected as good lipases producers. Lipases obtained through submerged fermentation presented optimal activities at 37°C and pH 7.2 and those obtained through solid-state fermentation at 35°C and pH 6.0. The enzymes produced by submerged fermentation were more temperature-stable than those obtained by solid-state fermentation, presenting 72% of residual activity after one hour of exposition at 90°C. Lipases obtained through submerged fermentation had 80% of stability in acidic pH and those obtained through solid-state fermentation had stability greater than 60% in alkaline pH.

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

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