scholarly journals Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles

2022 ◽  
Author(s):  
Rabaa Algotiml ◽  
Ali Gab-alla ◽  
Roshdi Seoudi ◽  
Hussein H. Abulreesh ◽  
Iqbal Ahmad ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Gold Nanoparticles ◽  
Cell Lines ◽  
Anticancer Agents ◽  
Chemical Constituents ◽  
Algal Species ◽  
Artemia Salina ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma

Biosynthesis of gold nanoparticles (AuNPs) is emerging as a better alternative to traditional chemical-based techniques. During this study, extracts of different marine algae species Ulva rigida (green algae), Cystoseira myrica (brown Algae), and Gracilaria foliifera (red Algae) were utilized as reducing and capping agents to synthesize AuNPs. AuNPs capped by U. rigida, C. myrica, and G. foliifera were confirmed by the appearance of surface plasmonic bands at 528, 540, and 543 nm, respectively. Transmission electron microscopy revealed mostly spherical shapes of AuNPs having a size of about 9 nm, 11 nm, and 13 nm for C. myrica, and G. foliifera extracts, respectively. Fourier transform-infrared spectroscopy (FTIR) illustrated the major chemical constituents of U. rigida, C. myrica, and G. foliifera. LC50 values of the biosynthesized AuNPs against Artemia salina nauplii were calculated at a range of concentrations (5-188 μg ml−1) after 16 to 24h. AuNPs concentration-dependent lethality was noted and U. rigida extracts-mediated AuNPs presented the lowest cytotoxicity. The biosynthesized AuNPs exhibited significant anticancer activity (86.83%) against MCF-7 cell lines (human breast adenocarcinoma cell lines) at 188 µg/ml concentration. G. foliifera demonstrated the highest anticancer value (92.13%) followed by C. myrica (89.82%), and U. rigida (86.83%), respectively. The AuNPs synthesized by different algal extracts showed variable antimicrobial activity against the tested pathogenic microorganisms. AuNPs of U. rigida extracts showed significant antimicrobial activity against dermatophytic fungi Trichosporon cataneum (30 mm) followed by Trichophyton mantigrophytes (25 mm). Furthermore, it also exhibited mild activity against Escherichia coli (17 mm), Cryptococcus neoformans (15 mm), Candida albicans (13 mm), and Staphylococcus aureus (11mm), respectively whereas no effects were observed against Bacillus cereus. To conclude, AuNPs can be effectively synthesized by marine algal species, and particularly U. rigida extracts could be effective reducing agents for the green AuNPs synthesis. These AuNPs could potentially serve as efficient alternative anticancer agents against human breast adenocarcinoma and anti-dermatophytes associated with skin infections.

Trans- and cis-2-phenylindole platinum(II) complexes as cytotoxic agents against human breast adenocarcinoma cell lines

2013 ◽  
Vol 1048 ◽  
pp. 88-97 ◽  
Author(s):  
Maria Tomé ◽  
Concepción López ◽  
Asensio González ◽  
Bahadir Ozay ◽  
Josefina Quirante ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cytotoxic Agents ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma

The expression of the chemokine receptor CXCR3 and its ligand, CXCL10, in human breast adenocarcinoma cell lines

2004 ◽  
Vol 92 (1-2) ◽  
pp. 171-178 ◽  
Author(s):  
Lilach Goldberg-Bittman ◽  
Eran Neumark ◽  
Orit Sagi-Assif ◽  
Elina Azenshtein ◽  
Tsipi Meshel ◽  
...  
Keyword(s):  
Cell Lines ◽  
Chemokine Receptor ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma

Effect of gamma interferon on HLA class-I and -II transcription and protein expression in human breast adenocarcinoma cell lines

1990 ◽  
Vol 45 (6) ◽  
pp. 1169-1176 ◽  
Author(s):  
Nabila Jabrane-Ferrat ◽  
Annick Faille ◽  
Pascale Loiseau ◽  
Odette Poirier ◽  
Dominique Charron ◽  
...  
Keyword(s):  
Protein Expression ◽  
Cell Lines ◽  
Hla Class I ◽  
Gamma Interferon ◽  
Class I ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma

Biofabrication of gold nanoparticles and its biocompatibility in human breast adenocarcinoma cells (MCF-7)

2014 ◽  
Vol 20 (4) ◽  
pp. 1713-1719 ◽  
Author(s):  
Muralidharan Murugan ◽  
Kevin John Pulikotil Anthony ◽  
Muniyandi Jeyaraj ◽  
Navanietha Krishnaraj Rathinam ◽  
Sangiliyandi Gurunathan
Keyword(s):  
Gold Nanoparticles ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Mcf 7

scholarly journals Human breast adenocarcinoma cell lines promote angiogenesis by providing cells with uPA-PAI-1 and by enhancing their expression

2002 ◽  
Vol 100 (5) ◽  
pp. 501-506 ◽  
Author(s):  
Khalid Bajou ◽  
Jean-Marc Lewalle ◽  
Christian Rodriguez Martinez ◽  
Claudine Soria ◽  
He Lu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Pai 1

scholarly journals Induction of Apoptosis by Gluconasturtiin-Isothiocyanate (GNST-ITC) in Human Hepatocarcinoma HepG2 Cells and Human Breast Adenocarcinoma MCF-7 Cells

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1240
Author(s):  
Asvinidevi Arumugam ◽  
Muhammad Din Ibrahim ◽  
Saie Brindha Kntayya ◽  
Nooraini Mohd Ain ◽  
Renato Iori ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Cell Lines ◽  
Chromatin Condensation ◽  
Time Dependent ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Gluconasturtiin, a glucosinolate present in watercress, is hydrolysed by myrosinase to form gluconasturtiin-isothiocyanate (GNST-ITC), which has potential chemopreventive effects; however, the underlying mechanisms of action have not been explored, mainly in human cell lines. The purpose of the study is to evaluate the cytotoxicity of GNST-ITC and to further assess its potential to induce apoptosis. GNST-ITC inhibited cell proliferation in both human hepatocarcinoma (HepG2) and human breast adenocarcinoma (MCF-7) cells with IC50 values of 7.83 µM and 5.02 µM, respectively. Morphological changes as a result of GNST-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GNST-ITC in a time-dependent manner. To delineate the mechanism of apoptosis, cell cycle arrest and expression of caspases were studied. GNST-ITC induced a time-dependent G2/M phase arrest, with reduction of 82% and 93% in HepG2 and MCF-7 cell lines, respectively. The same treatment also led to the subsequent expression of caspase-3/7 and -9 in both cells demonstrating mitochondrial-associated cell death. Collectively, these results reveal that GNST-ITC can inhibit cell proliferation and can induce cell death in HepG2 and MCF-7 cancer cells via apoptosis, highlighting its potential development as an anticancer agent.

scholarly journals Anti-proliferative Activity and Apoptotic Potential of Britannin, a Sesquiterpene Lactone from Inula aucheriana

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Maryam Hamzeloo Moghadam ◽  
Homa Hajimehdipoor ◽  
Soodabeh Saeidnia ◽  
Azadeh Atoofi ◽  
Roxana Shahrestani ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Sesquiterpene Lactone ◽  
Cell Lines ◽  
Mtt Assay ◽  
Proliferative Activity ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Inula aucheriana n-hexane, CHCl3 and MeOH extracts were evaluated for their anti-proliferative activity against HepG-2, MCF-7, MDBK and A-549 cells. The CHCl3 extract exhibited cytotoxic activity to the above cell lines with IC50 values of 13.5, 23.4, 10.5, and 26.9 μg/mL, respectively. The sesquiterpene lactone britannin was isolated from the above extract. This was further evaluated in the MTT assay to demonstrate strong cytotoxicity to the mentioned cell lines (IC50: 2.2, 5.9, 5.4, and 3.5 μg/mL, respectively), and the apoptotic inducing properties of britannin were evaluated on human breast adenocarcinoma (MCF-7) cells through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.

Sign in / Sign up

Export Citation Format

Share Document