scholarly journals Induction of Apoptosis by Gluconasturtiin-Isothiocyanate (GNST-ITC) in Human Hepatocarcinoma HepG2 Cells and Human Breast Adenocarcinoma MCF-7 Cells

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1240
Author(s):  
Asvinidevi Arumugam ◽  
Muhammad Din Ibrahim ◽  
Saie Brindha Kntayya ◽  
Nooraini Mohd Ain ◽  
Renato Iori ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Death ◽  
Cell Lines ◽  
Chromatin Condensation ◽  
Time Dependent ◽  
Breast Adenocarcinoma ◽  
Dependent Manner ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Gluconasturtiin, a glucosinolate present in watercress, is hydrolysed by myrosinase to form gluconasturtiin-isothiocyanate (GNST-ITC), which has potential chemopreventive effects; however, the underlying mechanisms of action have not been explored, mainly in human cell lines. The purpose of the study is to evaluate the cytotoxicity of GNST-ITC and to further assess its potential to induce apoptosis. GNST-ITC inhibited cell proliferation in both human hepatocarcinoma (HepG2) and human breast adenocarcinoma (MCF-7) cells with IC50 values of 7.83 µM and 5.02 µM, respectively. Morphological changes as a result of GNST-ITC-induced apoptosis showed chromatin condensation, nuclear fragmentation, and membrane blebbing. Additionally, Annexin V assay showed proportion of cells in early and late apoptosis upon exposure to GNST-ITC in a time-dependent manner. To delineate the mechanism of apoptosis, cell cycle arrest and expression of caspases were studied. GNST-ITC induced a time-dependent G2/M phase arrest, with reduction of 82% and 93% in HepG2 and MCF-7 cell lines, respectively. The same treatment also led to the subsequent expression of caspase-3/7 and -9 in both cells demonstrating mitochondrial-associated cell death. Collectively, these results reveal that GNST-ITC can inhibit cell proliferation and can induce cell death in HepG2 and MCF-7 cancer cells via apoptosis, highlighting its potential development as an anticancer agent.

Enhancement of docetaxel-treated MCF-7 cell death by 900-MHz radiation

2013 ◽  
Vol 8 (4) ◽  
pp. 357-365
Author(s):  
Marianna Trebuňová ◽  
Galina Laputková ◽  
Imrich Géci ◽  
Igor Andrašina ◽  
Ján Sabo
Keyword(s):  
Cell Culture ◽  
Cell Death ◽  
Electromagnetic Field ◽  
High Frequency ◽  
Input Power ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
High Frequency Electromagnetic Field ◽  
Mcf 7

AbstractThe aim of the study was to investigate the effect of high-frequency electromagnetic field of 900 MHz at 8 W input power on metabolic activity of human breast adenocarcinoma MCF-7 cells. With the aid of the colorimetric MTT assay, it was shown that there is significant change in cell culture survival exposed to docetaxel in field-free conditions in comparison with cells treated with docetaxel simultaneously exposed to high-frequency electromagnetic field.

scholarly journals Pit-1/GHF-1 and GH expression in the MCF-7 human breast adenocarcinoma cell line

2002 ◽  
Vol 173 (1) ◽  
pp. 161-167 ◽  
Author(s):  
C Gil-Puig ◽  
M Blanco ◽  
T Garcia-Caballero ◽  
C Segura ◽  
R Perez-Fernandez
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Rt Pcr ◽  
Adenocarcinoma Cell Line ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

GH expression in mammary tumors has been related to the increase and spreading of cell proliferation. Using the MCF-7 human breast adenocarcinoma cell line, it has been demonstrated that autocrine GH-stimulated mammary carcinoma cell proliferation decreased the apoptosis rate and enhanced cell spreading. Surprisingly, no data are available about the presence of Pit-1 (the main pituitary regulator of GH) or GH expression in this cell line. Using RT-PCR, Western blot and immunohistochemistry, we have demonstrated the presence of both mRNA coding Pit-1 and GH as well as Pit-1 and GH protein in the MCF-7 cell line. These data could imply that Pit-1 may be an adequate target to inhibit breast cell proliferation.

scholarly journals Anti-proliferative Activity and Apoptotic Potential of Britannin, a Sesquiterpene Lactone from Inula aucheriana

2012 ◽  
Vol 7 (8) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Maryam Hamzeloo Moghadam ◽  
Homa Hajimehdipoor ◽  
Soodabeh Saeidnia ◽  
Azadeh Atoofi ◽  
Roxana Shahrestani ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Sesquiterpene Lactone ◽  
Cell Lines ◽  
Mtt Assay ◽  
Proliferative Activity ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Inula aucheriana n-hexane, CHCl3 and MeOH extracts were evaluated for their anti-proliferative activity against HepG-2, MCF-7, MDBK and A-549 cells. The CHCl3 extract exhibited cytotoxic activity to the above cell lines with IC50 values of 13.5, 23.4, 10.5, and 26.9 μg/mL, respectively. The sesquiterpene lactone britannin was isolated from the above extract. This was further evaluated in the MTT assay to demonstrate strong cytotoxicity to the mentioned cell lines (IC50: 2.2, 5.9, 5.4, and 3.5 μg/mL, respectively), and the apoptotic inducing properties of britannin were evaluated on human breast adenocarcinoma (MCF-7) cells through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.

scholarly journals Synthesis of Novel Pyridopyridazin-3(2H)-one Derivatives and Evaluation of Their Cytotoxic Activity against MCF-7 Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Periasamy Selvakumar ◽  
Sathiah Thennarasu ◽  
Asit Baran Mandal
Keyword(s):  
Cell Proliferation ◽  
Cytotoxic Activity ◽  
Intramolecular Cyclization ◽  
Amide Proton ◽  
Breast Adenocarcinoma ◽  
Moderate Activity ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Derivatives Of ◽  
Mcf 7

A series of pyridopyridazin-3(2H)-one derivatives was synthesized in two facile steps. Mannich-type three-component condensation afforded the 2,6-diaryl piperidin-4-one derivatives, which underwent intramolecular cyclization in the presence of hydrazine or phenylhydrazine to yield the corresponding pyridopyridazin-3(2H)-one derivatives. All the derivatives of pyridopyridazin-3(2H)-one, except 3e and 3f, showed moderate activity against human breast adenocarcinoma (MCF-7) cells. The higher degree of inhibition of MCF-7 cell proliferation shown by 2a–2f indicates the significance of the amide proton in pyridopyridazin-3(2H)-one derivatives.

scholarly journals Calophyllum brasiliense Extracts Induced Apoptosis in Human Breast Adenocarcinoma Cells

2021 ◽  
pp. 50-64
Author(s):  
Michelle S. F. Correia ◽  
Anuska M. Alvares-Saraiva ◽  
Elizabeth C. P. Hurtado ◽  
Mateus L. B. Paciencia ◽  
Fabiana T. C. Konno ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Death ◽  
Breast Cancer Cell ◽  
Breast Adenocarcinoma ◽  
Apoptosis Induction ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Calophyllum Brasiliense ◽  
Apoptosis And Necrosis ◽  
Mcf 7

Aims: Apoptosis, or programmed cell death, is linked to several mechanisms of cell growth control. The present work aimed at evaluating the induction of apoptosis in MCF-7 human breast adenocarcinoma cell by Calophyllum brasiliense. Study design: The tests were performed in triplicates in the apoptosis assays and sextuplicates in the cytotoxic assays, to each group, and the data expressed the mean +/- standard deviations. The cytotoxicity IC50s were obtained based on nonlinear regression curve fit. Two-way ANOVA and Tukey’s tests were applied in the apoptosis analyses. Place and duration of study: The work was done at the Center for Research in Biodiversity (Cell Culture Laboratory, and Phytochemistry Laboratory), and Research Center (Molecular Biology Laboratory), Paulista University, between Jan 2019 and Dec 2019. Methodology: Two aqueous extracts, obtained from the stem (STE) and from the leaves (LFE) of Calophyllum brasiliense by a 24-h maceration, were submitted to a cytotoxic assay against MCF-7 breast cancer cell lines at the concentrations of 0.01 µg/ml, 0.1 µg/ml, 1.0 µg/ml, 10 µg/ml and 100 µg/ml. They were also subjected to the evaluation of apoptosis and necrosis cell death induction at concentrations of 50, 100 and 200 μg/ml, after 6 h, 12 h and 24 h. Curcumin was used as a reference drug for both cytotoxic (50 mM, 5.0 mM, 0.5 mM, 0.05 mM and 0.005 mM ) and apoptosis/necrosis (12.5 μM, 25 μM and 50 μM / 6 h, 12 h and 24 h) assays. Apoptosis and necrosis were accessed by the use of annexin V and 7-AAD, in a two-channel flow cytometer. Results: In terms of the cytotoxic activity, STE (IC50 7.86 µg/ml) was more toxic than LFE (IC50 74.35 µg/ml), and curcumin IC50 was 0.00159 µg/ml. STE induced 21.19 % and LFE, 20.63 %, in comparison to 13.4% of apoptosis induction by curcumin. The results of apoptosis induction in the cancer cells were achieved at 24 h, extract concentrations at 100 µg/ml. Conclusion: Both the extracts, STE and LFE, were cytotoxic against MCF-7 breast cancer cell line, and induced more apoptosis in MCF-7 cells than curcumin, suggesting that they are high potential sources of natural product-inducing apoptosis agents to be used against adenocarcinoma breast cells.

scholarly journals Induction of Apoptosis and Cytotoxicity by Raphasatin in Human Breast Adenocarcinoma MCF-7 Cells

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3092 ◽  
Author(s):  
Muhammad Ibrahim ◽  
Saie Kntayya ◽  
Nooraini Mohd Ain ◽  
Renato Iori ◽  
Costas Ioannides ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Chromatin Condensation ◽  
Annexin V ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Cycle Arrest ◽  
Induction Of Apoptosis ◽  
Mcf 7

Glucoraphasatin (GRH), a glucosinolate present abundantly in the plants of the Brassicaceae family, is hydrolyzed by myrosinase to raphasatin, which is considered responsible for its cancer chemopreventive activity; however, the underlying mechanisms of action have not been investigated, particularly in human cell lines. The aims of this study are to determine the cytotoxicity of raphasatin, and to evaluate its potential to cause apoptosis and modulate cell cycle arrest in human breast adenocarcinoma MCF-7 cells. The cytotoxicity was determined following incubation of the cells with glucoraphasatin or raphasatin (0–100 µM), for 24, 48, and 72 h. GRH displayed no cytotoxicity as exemplified by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. When myrosinase was added to the incubation system to convert GRH to raphasatin, cytotoxicity was evident. Exposure of the cells to raphasatin stimulated apoptosis, as was exemplified by cell shrinkage, membrane blebbing, chromatin condensation, and nuclear fragmentation. Moreover, using Annexin V-FITC assay, raphasatin induced apoptosis, as witnessed by changes in cellular distribution of cells, at different stages of apoptosis; in addition, raphasatin caused the arrest of the MCF-7 cells at the G2 + M phase. In conclusion, raphasatin demonstrated cancer chemopreventive potential against human breast adenocarcinoma (MCF-7) cells, through induction of apoptosis and cell cycle arrest.

scholarly journals Cytotoxicity of Manganese (III) Complex in Human Breast Adenocarcinoma Cell Line Is Mediated by the Generation of Reactive Oxygen Species Followed by Mitochondrial Damage

2016 ◽  
Vol 35 (6) ◽  
pp. 672-682 ◽  
Author(s):  
Qudes Al-anbaky ◽  
Zeiyad Al-karakooly ◽  
Surya P. Kilaparty ◽  
Megha Agrawal ◽  
Yahya M. Albkuri ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Breast Adenocarcinoma ◽  
Oxygen Species ◽  
Human Breast ◽  
Adenocarcinoma Cell Line ◽  
Cytotoxic Effects ◽  
Adenocarcinoma Cell ◽  
Human Breast Adenocarcinoma ◽  
Mcf 7

Manganese (Mn) complexes are widely studied because of their important catalytic properties in synthetic and biochemical reactions. A Mn (III) complex of an amidoamine ligand was synthesized using a tetradentate amidoamine ligand. In this study, the Mn (III) complex was evaluated for its biological activity by measuring its cytotoxicity in human breast adenocarcinoma cell line (MCF-7). Cytotoxic effects of the Mn (III) complex were determined using established biomarkers in an attempt to delineate the mechanism of action and the utility of the complex as a potential anticancer drug. The Mn (III) complex induces cell death in a dose- and time-dependent manner as shown by microculture tetrazolium assay, a measure of cytotoxic cell death. Our results demonstrated that cytotoxic effects were significantly increased at higher concentrations of Mn (III) complex and with longer time of treatment. The IC50 (Inhibitor concentration that results in 50% cell death) value of Mn (III) complex in MCF-7 cells was determined to be 2.5 mmol/L for 24 hours of treatment. In additional experiments, we determined the Mn (III) complex–mediated cell death was due to both apoptotic and nonspecific necrotic cell death mechanisms. This was assessed by ethidium bromide/acridine orange staining and flow cytometry techniques. The Mn (III) complex produced reactive oxygen species (ROS) triggering the expression of manganese superoxide dismutase 1 and ultimately damaging the mitochondrial function as is evident by a decline in mitochondrial membrane potential. Treatment of the cells with free radical scavenger, N, N-dimethylthiourea decreased Mn (III) complex–mediated generation of ROS and attenuated apoptosis. Together, these results suggest that the Mn (III) complex–mediated MCF-7 cell death utilizes combined mechanism involving apoptosis and necrosis perhaps due to the generation of ROS.

scholarly journals ISOLATION, OPTIMIZATION, AND ANTITUMOR ACTIVITY OF L-ASPARAGINASE EXTRACTED FROM PECTOBACTERIUM CAROTOVORUM AND SERRATIA MARCESCENS ON HUMAN BREAST ADENOCARCINOMA AND HUMAN HEPATOCELLULAR CARCINOMA CANCER CELL LINES

2019 ◽  
pp. 332-337
Author(s):  
NOHA E ABDEL-RAZIK ◽  
KHALED Z EL-BAGHDADY ◽  
EINAS H EL-SHATOURY ◽  
NAHLA G MOHAMED
Keyword(s):  
Hepatocellular Carcinoma ◽  
Antitumor Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Breast Adenocarcinoma ◽  
Pectobacterium Carotovorum ◽  
Human Breast ◽  
Human Breast Adenocarcinoma ◽  
Diphenyl Tetrazolium Bromide ◽  
Mcf 7

Objectives: The objective of this research was to obtain isolates capable of producing a high yield of L-asparaginase enzyme and to evaluate the antitumor activity of the purified enzyme against different cancer and normal cell lines. Methods: Isolation of bacteria was performed by the serial dilution technique of soil samples collected from Cairo, Egypt, using modified M9 agar plates. Culture filtrates of selected isolates were quantitatively screened for L-asparaginase production using well-diffusion and direct nesslerization techniques. Factors influencing L-asparaginase activity were optimized by studying the effect of physical and nutritional conditions on the enzyme activity. The purification of L-asparaginase extracted from both the isolates was achieved using chilled acetone (−20°C), followed by gel filtration on Sephadex G-100. The anticancer activity of the purified enzyme against human breast adenocarcinoma (MCF-7), human hepatocellular carcinoma (HepGII) and homo sapiens human (WISH) cell line was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay. Results: Two L-asparaginase producers were identified by Biolog identification system as Pectobacterium carotovorum and Serratia marcescens. Optimization increased the production of L-asparaginase to 4.835 and 5.221 U/ml for P. carotovorum and S. marcescens, respectively. L-asparaginase was extracted, purified, and tested in vitro for cytotoxic activity using 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT assay) against MCF-7, HepGII, and WISH cell line. L-asparaginase from P. carotovorum and S. marcescens was neutral to normal epithelial WISH cells. On the other hand, L-asparaginase from both isolates was cytotoxic to MCF-7 and HepGII cancer cell lines with an half maximal inhibitory concentration of 15 μg/ml and 26 μg/ml and 26 μg/ml and 25 μg/ml, respectively. Conclusion: L-asparaginase extracted from P. carotovorum and S. marcescens showed remarkable anticancer activity. Further studies on hypersensitivity action need to be carried out to recommend the use of L-asparaginase as an alternative to commercially available preparations.

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