In Vitro Asymbiotic Seed Germination and Propagation of Polystachya concreta (Jacq) Garay & H.R Sweet, An Important Medicinal Orchid

Effects of different nutrient media, vitamins and peptone on in vitro asymbiotic seed germination of Geodorum densiflorum (Lam.) Schltr. were studied. In vitro developed rhizomes were used to determine the influence of cytokinins on growth and morphogenesis. Seed germination and survival rate of protocorms were highest in MS medium compared to other basal media. The protocorms raised through seed germination directly proliferated into rhizomes in later phase of growth and development. Nodal portions of rhizomes further exhibited growth through formation of direct and callus mediated protocorm like bodies. In general, three types of rhizome movements were noticed; viz. positively geotropic, negatively geotropic and diagravitropic movements. In the PGR free control and in the presence of NAA alone rhizomes exhibited positively geotropic movements. On the contrary, presence of cytokinin either alone or in combination with NAA exhibited diagravitropic movements. Application of TDZ completely suppressed the positively geotropic movement and enhanced the frequency of negatively geotropic movement of rhizomes followed by shoot bud formation. BAP was most effective cytokinin for large scale rhizome mediated multiplication of Geodorum densiflorum and TDZ could be effectively employed for rapid leafy shoot regeneration. Plant Tissue Cult. & Biotech. 30(1): 65-75, 2020 (June)

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In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v31i2.57343 ◽

2022 ◽

Vol 31 (2) ◽

pp. 153-160

Author(s):

Saranjeet Kaur

Keyword(s):

Seed Germination ◽

Plant Tissue ◽

Activated Charcoal ◽

In Vitro Propagation ◽

In Vitro Conservation ◽

Coconut Water ◽

Asymbiotic Seed Germination ◽

Orchid Species ◽

Medicinal Orchid

The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 μM, BAP - 4.44 μM), and auxin (NAA- 5.37 μM). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication of protocorm like bodies. Activated charcoal successfully checked the release of brownish exudates in the cultures. Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)

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In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v3i2.12476 ◽

2015 ◽

Vol 3 (2) ◽

pp. 243-247 ◽

Cited By ~ 2

Author(s):

Varsha Dawande ◽

Rajaram Gurav

Keyword(s):

Seed Germination ◽

Root Growth ◽

Growth Regulators ◽

Subsequent Development ◽

Ms Medium ◽

Asymbiotic Seed Germination ◽

Endangered Orchid ◽

Maximum Root ◽

Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476

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In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

Scientific World ◽

10.3126/sw.v9i9.5518 ◽

1970 ◽

Vol 9 (9) ◽

pp. 50-52 ◽

Cited By ~ 3

Author(s):

Bijaya Pant ◽

Sumitra Shrestha ◽

Shreeti Pradhan

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Seedling Development ◽

Naphthalene Acetic Acid ◽

Ideal Condition ◽

Ms Medium ◽

Mass Propagation ◽

Asymbiotic Seed Germination ◽

Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

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An effective nutrient medium for asymbiotic seed germination and large-scale in vitro regeneration of Dendrobium hookerianum, a threatened orchid of northeast India

AoB Plants ◽

10.1093/aobpla/plr032 ◽

2011 ◽

Vol 2012 ◽

Cited By ~ 12

Author(s):

Sumi Paul ◽

Suman Kumaria ◽

Pramod Tandon

Keyword(s):

Seed Germination ◽

Nutrient Medium ◽

Large Scale ◽

In Vitro Regeneration ◽

Northeast India ◽

Asymbiotic Seed Germination

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Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

The Scientific World JOURNAL ◽

10.1155/2014/740150 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 9

Author(s):

Potshangbam Nongdam ◽

Leimapokpam Tikendra

Keyword(s):

Acetic Acid ◽

Seed Germination ◽

Germination Percentage ◽

Naphthalene Acetic Acid ◽

Mass Propagation ◽

Asymbiotic Seed Germination ◽

Orchid Species ◽

Seed Culture ◽

Leaf Formation

An efficientin vitroregeneration protocol from seed culture has been established successfully forDendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated morein vitrorooting, though IBA was found to be more effective in rooting induction as compared to NAA. Thein vitroprotocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly importantDendrobiumorchid species.

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