scholarly journals In-Vitro Seed Germination and Effect of Growth Regulators on Subsequent Development of Protocorms of Eulophia Nuda Lindl

2015 ◽  
Vol 3 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Varsha Dawande ◽  
Rajaram Gurav
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Growth Regulators ◽  
Subsequent Development ◽  
Ms Medium ◽  
Asymbiotic Seed Germination ◽  
Endangered Orchid ◽  
Maximum Root ◽  
Regenerated Plantlets

Asymbiotic seed germination of Eulophia nuda Lindl. was observed on Knudson C medium. About 90% seeds germinated within 8-10 weeks and formed green protocorms in 11-12 weeks. Effect of BA and IBA was studied on plantlet development from protocorms. BA shows the best results with respect to number and length of shoots. Maximum number (6.45±1.36) and length (3.90±0.99) was observed on MS medium supplemented with 4.44μM.BA. Maximum root growth was also observed on same medium (4.8±0.99 number of roots and 1.43±0.13cm length). The regenerated plantlets were successfully acclimatized and transferred to earthen pots. The results presented here show that in vitro seed germination and plantlet development in Eulophia nuda Lindl., an endangered orchid, can be achieved at a higher rate by this method.Int J Appl Sci Biotechnol, Vol 3(2): 243-247 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12476   

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals The influence of light spectra, UV-A, and growth regulators on the in vitro seed germination of Senecio cineraria DC.

Revista CERES ◽  
2010 ◽  
Vol 57 (5) ◽  
pp. 576-580 ◽  
Author(s):  
Cristiane Pimentel Victório ◽  
Nina Cláudia Barbosa da Silva ◽  
Maria Apparecida Esquibel ◽  
Alice Sato
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
White Light ◽  
Germination Rate ◽  
Germination Percentage ◽  
Ms Medium ◽  
In Vitro Germination ◽  
Light Spectra ◽  
Indole 3 Acetic Acid

This study was carried out to investigate the effects of light spectra, additional UV-A, and different growth regulators on the in vitro germination of Senecio cineraria DC. Seeds were surface-sterilized and inoculated in MS medium to evaluate the following light spectra: white, white plus UV-A, blue, green, red or darkness. The maximum germinability was obtained using MS0 medium under white light (30%) and MS + 0.3 mg L-1 GA3 in the absence of light (30.5%). S. cineraria seeds were indifferent to light. Blue and green lights inhibited germination. Different concentrations of gibberellic acid (GA3) (0.1; 0.4; 0.6; 0.8; 1.0 and 2.0 mg L-1) and indole-3-acetic acid IAA (0.1; 0.3 and 1.0 mg L-1) were evaluated under white light and darkness. No concentration of GA3 enhanced seed germination percentage under white light. However, when the seeds were maintained in darkness, GA3 improved germination responses in all tested concentrations, except at 1.0 mg L-1. Under white light, these concentrations also increased the germination time and reduced germination rate. Germination rate, under light or darkness, was lower using IAA compared with GA3.

scholarly journals In vitro regeneration potentiality of Brassica genotypes in differential growth regulators

1970 ◽  
Vol 35 (2) ◽  
pp. 189-199 ◽  
Author(s):  
MMA Khan ◽  
ABM Arif Hasan Khan Robin ◽  
MAN Nazim-Ud-Dowla ◽  
SK Talukder ◽  
L Hassan
Keyword(s):  
Plant Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Root Formation ◽  
Ms Medium ◽  
Differential Growth ◽  
The Media ◽  
Regenerated Plantlets

Petiole of six genotypes of oilseed Brassica viz. Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8, and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA, and AgNO3 for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 in the media supplemented with 2 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS media supplemented with 2.5 mg/L BAP, 0.1 mg/L NAA and 2.0 mg/L AgNO3. Root formation from the regenerants was found best in half MS medium supplemented with 0.5 mg/L NAA in genotype Tori-7. Regenerated plantlets of four genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.Keywords: AgNO3; BAP; Brassica; NAA; regeneration.  DOI: 10.3329/bjar.v35i2.5881Bangladesh J. Agril. Res. 35(2) : 189-199, June 2010

scholarly journals Asymbiotic seed germination and in vitro propagation of Cattleya trianae Linden & Reichb.f. (Orchidaceae)

2017 ◽  
Vol 66 (4) ◽  
pp. 544-548 ◽  
Author(s):  
Seir Antonio Salazar Mercado ◽  
Nelson Alfonso Vega Contreras
Keyword(s):  
Seed Germination ◽  
Threatened Species ◽  
Growth And Development ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Ms Medium ◽  
Organic Additives ◽  
Natural Habitats ◽  
Asymbiotic Seed Germination

Cattleya trianae (Linden & Reichb.f., 1860), Colombian national flower, is in danger of extinction due to the destruction of its natural habitats and excessive collection for horticultural purposes. Therefore, in vitro culture is a tool for the conservation of threatened species. In this study we determined the most suitable culture medium for asimbytic seed germination and in vitro propagation of C. trianae. Initially, mature capsules were collected, the seeds were subsequently disinfected and seeded with the syringe method (Vendrame et al., 2007), to evaluate the effect of five media on the development of C. trianae after 20 weeks. The seedlings were transplanted and acclimated using different substrates. The best percentage (54.2%) of seedling formation after 20 weeks was found in MS + JP medium with significant differences (P <0.05: Tukey HSD). In this research, it is reported that the addition of organic additives to the MS medium improves the efficacy of this, and therefore, allows a greater growth and development of C. trianae under in vitro conditions.

scholarly journals Perbanyakan Anggrek Spesies Paphiopedilum glaucophyllum J.J.Smith melalui Proliferasi Tunas Adventif Secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
pp. 115
Author(s):  
Tubagus Kiki Kawakibi Azmi ◽  
Ni Made Armini Wiendi
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Factorial Design ◽  
Key Words ◽  
Callus Induction ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Ms Medium ◽  
Optimum Growth

<p>ABSTRACT</p><p>This research was  aimed  to determine the effects of  BAP and medium on adventitious  shoot proliferation of  Paphiopedilum  glaucophyllum  J.J.  Smith in vitro. Plantlet of 1 year 9 months old from  seed  germination  in  vitro  on modified  Knudson  C  medium  was  used  as  an  explant.  This research was arranged in a Factorial Design with three  replications. The first factor was different concentration of BAP, consisting  of 1 and 2 mg L-1(All combination medium were added with 0.5 mg L-12.4-D). The second factor was different concentration of macro and micro nutrient from MS (Murashige and Skoog) and KC (Knudson C) medium, consisting  of 1, ¾, ½, and ¼ concentrationsof macro and  micro  nutrient.  The  result  showed  that  plantlets  failed  to  response to  the  entireadventitious  shoot  proliferation  combination  medium  within 16 weeks.  Concentration  of  medium affected to  leaves and root growth. The optimum growth was achieved at ¾ concentration of macro and micro nutrient from KC medium, combined with 2 mg L-1BAP for leaves growth and 1 mg L-1BAP for root growth. BAP affected callus  induction. The highest percentage of callus induction  was achieved at 1 mg L-1BAP (48.61%).</p><p>Key words: BAP, KC, MS, medium, proliferation.</p><p> </p><p>ABSTRAK</p><p>Penelitian dilakukan untuk mempelajari pengaruh zat pengatur tumbuh BAP dan media MS (Murashige  &amp;  Skoog)  dan  KC  (Knudson  C)  terhadap kemampuan proliferasi  tunas  adventif Paphiopedilum  glaucophyllum  J.J.Smith  secara  in vitro.  Bahan  tanaman  yang  digunakan  adalah planlet hasil pengecambahan biji secara in vitro  yang telah berumur 1 tahun 9 bulan yang  diperoleh dari Pusat Konservasi Tumbuhan,  Kebun Raya,  Bogor.  Penelitian  menggunakan Rancangan Acak Lengkap  (RAL)  dengan  dua  faktor  perlakuan  yang  disusun secara  faktorial  dengan  tiga  ulangan. Faktor  pertama  adalah  BAP  yang terdiri  dari  1  dan  2  mg  L-1,  penggunaan  BAP  dikombinasikan dengan 0.5 mg L-1 2.4-D. Faktor kedua adalah media (MS dan KC) dengan konsentrasi hara makro dan mikro masing-masing adalah 1, ¾, ½, dan ¼ konsentrasi. Hasil penelitian menunjukkan bahwa proliferasi  tunas  adventif  tidak  terjadi pada  semua  perlakuan.  Konsentrasi  media  (MS  dan  KC) berpengaruh nyata terhadap  pertumbuhan  daun  dan  akar.  Pertumbuhan  daun  dan  akar optimum diperoleh  pada  ¾  konsentrasi  hara  makro  dan  mikro  pada kedua jenis  media.  Pertumbuhan  daun tertinggi diperoleh pada media KC ¾ konsentrasi hara makro dan mikro ditambah 2 mg L-1. Media KC  ¾ konsentrasi hara  makro  dan  mikro  ditambah  1  mg  L-1menghasilkan  jumlah  total akar tertinggi.  BAP  berpengaruh  nyata  terhadap  persentase  planlet berkalus.  Jumlah  planlet  berkalus tertinggi diperoleh pada media yang mengandung 1 mg L-1 BAP (48.61%).</p><p>Kata kunci: BAP, KC, MS, media, proliferasi.</p>

scholarly journals An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum

Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 298
Author(s):  
Linling Yao ◽  
Jialin Huang ◽  
Shibao Zhang
Keyword(s):  
Seed Germination ◽  
Developmental Process ◽  
Seedling Development ◽  
Ms Medium ◽  
Seedling Regeneration ◽  
Asymbiotic Seed Germination ◽  
Differentiation Stage ◽  
Optimal Medium ◽  
Germination Stage

Paphiopedilum tigrinum is an endangered orchid with high ornamental value. However, seed germination and seedling regeneration in P. tigrinum is very difficult in vitro. Little is known about why P. tigrinum seedlings are difficult to propagate or how to improve the seed germination and seedling rates of this species. In this study, we investigated the developmental process of P. tigrinum from asymbiotic seed germination to seedling rooting by comparing it with P. appletoniantum, a much easier species for germination and seedling formation. We found that asymbiotic seed germination in P. tigrinum is limited by severe browning of the protocorm at the seed germination stage, and protocorm rooting at the differentiation stage was also proved to be difficult. The optimal medium for seed germination of P. tigrinum was a modified Harvais (mHa) medium supplemented with 0.5 mg·L−1 kinetin (Kin), 0.1 g·L−1 activated charcoal (AC) and 100 mL·L−1 coconut water (CW). At the protocorm differentiation stage, seedlings with 1–2 leaves were obtained on a 1/4 MS medium supplemented with 1.0 mg·L−1 6-benzylaminopurin (BA), 0.3 g·L−1 AC and 50–100 mL·L−1 CW after culturing for 120 day. At the seedling subculture stage, a 1/2 MS medium supplemented with 0.5–1.5 g·L−1 AC and 100 mL·L−1 CW was better for leaf and root growth of P. tigrinum. At the rooting stage, a 1/2 MS medium supplemented with 1.0 g·L−1 AC, 0.5 g·L−1 dolomite flour, 15 g·L−1 potato homogenate and 30 g·L−1 banana homogenate was most suitable for the growth and rooting of seedlings. This study has established an effective protocol for seed germination and seedling regeneration of P. tigrinum.

scholarly journals In Vitro Regeneration of Dendrobium sp. of Orchid Using Leaf Tip as Explant

2016 ◽  
Vol 8 (2) ◽  
pp. 75-78
Author(s):  
K Goswami ◽  
S Yasmin ◽  
KM Nasiruddin ◽  
F Khatun ◽  
J Akte
Keyword(s):  
In Vitro Regeneration ◽  
Plantlet Regeneration ◽  
Shoot Length ◽  
Ms Medium ◽  
Fresh Weight ◽  
Half Strength ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Regenerated Plantlets

Plant growth regulators (PGRs) namely, 2,4-D, NAA and BAP were added into Murashige and Skoog (MS) medium to observe the effect of PGRs on the growth and development of Dendrobium sp. orchid. Leaf tips of Dendrobium sp. were used as explants and inoculated on MS medium supplemented with 2, 4 D (0, 0.5, 2.5, 5, 10 mgL?1) for development of PLBs. The maximum PLBs formation (90%) and the maximum number of PLBs (16.00) were observed in 10 mgL?1 2, 4-D into MS medium after 60 days of culture. Subcultured PLBs were inoculated on MS medium supplemented with different combinations of NAA (0, 0.5, 2.5, 5 mgL?1) and BAP (0, 0.5, 2.5, 5 mgL?1) for shoot regeneration. The maximum number of shoot (11.00), the highest fresh weight (0.6233g) and the highest shoot length (3.613 cm) were observed in 0.5 mgL?1 NAA + 0.5 mgL?1 BAP after 60 days of culture. Even, the maximum number of root (4.00), the maximum root length (1.627cm) and the maximum plantlet regeneration percentage (93.33%) were observed with the combined effect of 0.5 mg NAA and 0.5 mg BAP after 60 days of culture. Finally, regenerated plantlets were transferred into half strength MS medium to obtain plants.J. Environ. Sci. & Natural Resources, 8(2): 75-78 2015

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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