scholarly journals Digital Lighting Technologies to Control Galleria Mellonella Behavior

2021 ◽  
Vol 15 (1) ◽  
pp. 78-83
Author(s):  
N. P. Kondrat'eva ◽  
D. V. Buzmakov ◽  
I. R. Il'yasov ◽  
R. G. Bol'shin ◽  
M. G. Krasnolutskaya
Keyword(s):  
Optical Radiation ◽  
Galleria Mellonella ◽  
Visual Programming ◽  
Automated System ◽  
Main Element ◽  
Microprocessor System ◽  
Radiation Characteristics ◽  
Greater Wax Moth ◽  
Radiation Sources ◽  
Wax Moth

The authors found out that the greater wax moth (Galleria mellonella) harmed bee colonies by destroying up to 30 percent of honey in a beehive. They studied the results of experiments on controlling its behavior using a developed automated microprocessor system that maintained optical radiation sources duration.(Research purpose) To develop light technologies using a microprocessor-based automated system that allows controlling Galleria mellonella behavior by realizing attractive optical radiation characteristics.(Materials and methods) Galleria mellonella behavior was controlled by an automated system that the authors worked out for maintaining the required duration of the experiment and optical radiation parameters. The ATmega328 microcontroller was chosen as the main element of the developed microprocessor automated system. The authors created a program for it using visual programming FLProg version 5.3.0.(Results and discussion) The authors found that, regardless of the experiment duration, Galleria mellonella butterflies prefered radiation with 400 nanometers wavelength.(Conclusions) The authors determined that radiation with 400 nanometers wavelength and 10 minutes duration was attractive. They showed the ability to control Galleria mellonella behavior, luring butterflies to a specific place in the beehive with comfortable spectrum parameters. An automated system for controlling the greater wax moth butterfly behavior was developed on ATmega328 microcontroller by implementing attractive optical radiation characteristics.

Digital Electrical Technologies to Control Insect Behavior

2020 ◽  
pp. 9-16
Author(s):  
Nadezhda P. Kondrat’eva ◽  
Daniil V. Buzmakov ◽  
Il’nur R. Il’yasov ◽  
Roman G. Bol’shin ◽  
Mariya G. Krasnolutskaya
Keyword(s):  
Optical Radiation ◽  
Galleria Mellonella ◽  
Environmentally Friendly ◽  
Insect Behavior ◽  
Visual Programming ◽  
Automated System ◽  
Research Purpose ◽  
Main Element ◽  
Automated Control ◽  
Wax Moth

One of the main pests of bee colonies is the large wax moth, which can destroy up to 30 percent of the honey in the hive. Controlling the behavior of living objects can be based on their positive or negative response to optical radiation characteristics such as spectrum and exposure. Many insects see ultraviolet radiation. Authors suggest using an environmentally friendly method to attract butterflies with optical radiation. (Research purpose) The research purpose is in developing digital electrical technologies using a microprocessor-based automated system that allows controlling the behavior of large wax moth butterflies (Galleria mellonella) by implementing attractive characteristics of optical radiation. (Materials and methods) Authors controlled the behavior of a large wax moth using a developed automated system for maintaining the required duration of the experiment and optical radiation parameters. The main element of the system is the ATmega328 microcontroller, the program for which was written using the visual programming of FLProg 5.3.0. (Results and discussion) The use of digital electrotechnologies using the developed microprocessor automated control system on the ATmega328 microcontroller and assembled on the Arduino Uno Board makes it possible to implement energy-saving environmentally friendly light electrotechnologies for controlling the behavior of large wax moth butterflies. Regardless of the duration of the experiment, Galleria mellonella butterflies prefer radiation with a wavelength of 400 nanometers. (Conclusions) The experiments confirm that the radiation with a wavelength of 400 nanometers lasting 10 minutes at a temperature of 32 degrees Celsius and a humidity of 60 percent was attractive. These comfortable spectrum parameters allow to control the behavior of Galleria Mellonella by luring butterflies to a specific location in the hive.

scholarly journals Effect of optical radiation on greater wax moth (Galleria mellonella L.) – pest of bee colonies

2020 ◽  
Vol 433 ◽  
pp. 012036
Author(s):  
N P Kondrateva ◽  
N K Kirillov ◽  
P V Zaitsev ◽  
D V Buzmakov ◽  
A S Osokina ◽  
...  
Keyword(s):  
Optical Radiation ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Bee Colonies ◽  
Wax Moth

scholarly journals Establishing an invertebrate Galleria mellonella greater wax moth larval model of Neisseria gonorrhoeae infection

Virulence ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 1900-1920
Author(s):  
Aiste Dijokaite ◽  
Maria Victoria Humbert ◽  
Emma Borkowski ◽  
Roberto M La Ragione ◽  
Myron Christodoulides
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Wax Moth

Transgenic expression of gallerimycin, a novel antifungal insect defensin from the greater wax moth Galleria mellonella, confers resistance to pathogenic fungi in tobacco

2006 ◽  
Vol 387 (5) ◽  
pp. 549-557 ◽  
Author(s):  
Gregor Langen ◽  
Jafargholi Imani ◽  
Boran Altincicek ◽  
Gernot Kieseritzky ◽  
Karl-Heinz Kogel ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Fungal Pathogens ◽  
Galleria Mellonella ◽  
Ectopic Expression ◽  
Pathogenic Fungi ◽  
Transgenic Expression ◽  
Greater Wax Moth ◽  
Insect Defensin ◽  
Wax Moth

Abstract A cDNA encoding gallerimycin, a novel antifungal peptide from the greater wax moth Galleria mellonella, was isolated from a cDNA library of genes expressed during innate immune response in the caterpillars. Upon ectopic expression of gallerimycin in tobacco, using Agrobacterium tumefaciens as a vector, gallerimycin conferred resistance to the fungal pathogens Erysiphe cichoracearum and Sclerotinia minor. Quantification of gallerimycin mRNA in transgenic tobacco by real-time PCR confirmed transgenic expression under control of the inducible mannopine synthase promoter. Leaf sap and intercellular washing fluid from transgenic tobacco inhibited in vitro germination and growth of the fungal pathogens, demonstrating that gallerimycin is secreted into intercellular spaces. The feasibility of the use of gallerimycin to counteract fungal diseases in crop plants is discussed.

Greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) as a resistant model host for Nosema pyrausta (Microsporidia: Nosematidae)

2018 ◽  
Vol 157 ◽  
pp. 1-3 ◽  
Author(s):  
Yuri S. Tokarev ◽  
Ekaterina V. Grizanova ◽  
Anastasia N. Ignatieva ◽  
Ivan M. Dubovskiy
Keyword(s):  
Galleria Mellonella ◽  
Greater Wax Moth ◽  
Lepidoptera Pyralidae ◽  
Wax Moth

scholarly journals Quantitative and Qualitative Impacts of Selected Arthropod Venoms on the Larval Haemogram of the Greater Wax Moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae)

2021 ◽  
pp. 1-26
Author(s):  
Karem Ghoneim ◽  
Khalid Hamadah ◽  
Mohammad Tanani ◽  
Dyaa Emam
Keyword(s):  
Apis Mellifera ◽  
Galleria Mellonella ◽  
Larval Instar ◽  
Population Count ◽  
Oriental Hornet ◽  
Greater Wax Moth ◽  
The World ◽  
Lepidoptera Pyralidae ◽  
Hemocyte Population ◽  
Wax Moth

The greater wax moth, Galleria mellonella (Linnaeus) (Lepidoptera: Pyralidae) is the most destructive pest of honey bee, Apis mellifera Linnaeus (Hymenoptera: Apidae), throughout the world. The present study was conducted to determine the quantitative and qualitative impairing effects of the arthropod venoms, viz., death stalker scorpion Leiurus quinquestriatus (Hemprich & Ehrenberg) venom (SV), oriental Hornet (wasp) Vespa orientalis Linnaeus venom (WV) and Apitoxin of A. mellifera (AP) on the larval haemogram. For this purpose, the 3rd instar larvae were treated with LC50 of each of these venoms (3428.9, 2412.6, and 956.16 ppm, respectively). The haematological investigation was conducted in haemolymph of the 5th and 7th (last) instar larvae. The important results could be summarized as follows. Five basic types of the freely circulating haemocytes in the haemolymph of last instar (7th) larvae of G. mellonella had been identified: Prohemocytes (PRs), Plasmatocytes (PLs), Granulocytes (GRs), Spherulocytes (SPs) and Oenocytoids (OEs). All venoms unexceptionally prohibited the larvae to produce normal hemocyte population (count). No certain trend of disturbance in the differential hemocyte counts of circulating hemocytes in larvae of G. mellonella after treatment with the arthropod venoms. Increasing or decreasing population of the circulating hemocytes seemed to depend on the potency of the venom, hemocyte type and the larval instar.  In PRs of last instar larvae, some cytopathological features had been observed after treatment with AP or WV, but SV failed to cause cytopathological features. With regard to PLs, some cytopathological features had been observed after treatment with AP while both SV and WV failed to cause cytopathological features in this hemocyte type. No venom exhibited cytopathological effects on GRs, SPs or OEs.

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