scholarly journals Ceramids as biomarkers of chronic periodontitis associated with type 2 diabetes

2020 ◽  
Vol 24 (4) ◽  
pp. 325-337
Author(s):  
K. G. Unanyan ◽  
I. P. Balmasova ◽  
V. N. Tsarev ◽  
A. M. Mkrtumyan ◽  
K. S. Elbekyan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Sphingolipid Metabolism ◽  
Systemic Effects ◽  
16S Sequencing ◽  
Periodontal Tissues ◽  
Ceramide Kinase

Relevance . The association of chronic periodontitis with type 2 diabetes mellitus is one of the most frequent manifestations of systemic effects that are etiologically associated with periodontopathogenic bacteria in the biofilm of the gingival sulcus. In this regard, the study of the metabolic mechanisms leading to such systemic effects and serving their markers is an urgent problem. Aim . Study of the features of sphingolipid/ceramide metabolism, both produced by etiologically significant microflora, and present in periodontal tissues of patients on the example of the association of chronic periodontitis with type 2 diabetes. Materials and methods . The observation groups included 58 patients with chronic periodontitis in association with type 2 diabetes, 39 patients with chronic periodontitis without concomitant systemic pathology, and 27 conditionally healthy people. All the examined patients underwent molecular genetic studies of the taxonomic and metabolic profiles of the dental sulcus/ periodontal pockets microbiota using 16S sequencing and evaluation of phosphorylated ceramides in saliva by the activity of the ceramid kinase enzyme. Results . It was found that in the Association of chronic periodontitis with type 2 diabetes mellitus, there are features of the taxonomic composition of the dental sulcus/periodontal pockets microbiota, which are combined with a decrease in sphingolipid metabolism. In addition, in these patients, depending on the duration of diabetes mellitus, there was an increasing drop in the saliva of ceramide kinase, which determines the phosphorylation of sphingolipids/ceramides. Conclusion . In the Association of chronic periodontitis with type 2 diabetes mellitus, the systemic effects of the dental sulcus/ periodontal pockets microbiota are manifested by a decrease in sphingolipid metabolism, including a decrease in ceramide kinase in periodontal tissues, which can serve as a marker of the combined pathological process.

scholarly journals “Shielding” of Cytokine Induction by the Periodontal Microbiome in Patients with Periodontitis Associated with Type 2 Diabetes Mellitus

Acta Naturae ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 79-87 ◽  
Author(s):  
I. P. Balmasova ◽  
Y. A. Lomakin ◽  
E. A. Babaev ◽  
V. N. Tsarev ◽  
A. G. Gabibov ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Systemic Effects ◽  
Human Immune System ◽  
Gm Csf ◽  
Human Immune

Periodontal diseases, especially those with polymicrobial etiology, are often associated with type 2 diabetes mellitus, proceeding more severely and affecting the course of diabetes mellitus. Recently, this feature has been associated with the ability of periodontopathogen microflora to cause not only a local infectious process in the oral cavity, but also to interact with the human immune system and induce various systemic effects. We investigated changes in the salivary cytokine profile of patients with chronic periodontitis, associated and not associated with type 2 diabetes mellitus. We observed a statistically significant decrease of MCP-1/CCL2, GM-CSF, IL-5, IL-6, and IFN- in the saliva of patients with chronic periodontitis associated with type 2 diabetes mellitus in comparison with patients with chronic periodontitis only. All of these cytokines are associated with macrophage activation. These data are an important contribution to the elucidation of the mechanism of periodontopathogens involvement in the manifestation of the systemic effects of type 2 diabetes.

Diagnostic value of microbiome biomarkers of the periodonite microbiome in patients with the association of chronic periodontitis and diabetes mellitus type 2

2021 ◽  
Vol 66 (11) ◽  
pp. 678-683
Author(s):  
I. P. Balmasova ◽  
V. N. Tsarev ◽  
K. G. Unanyan ◽  
E. V. Ippolitov ◽  
T. V. Tsareva ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Diagnostic Value ◽  
High Tech ◽  
Periodontal Tissues ◽  
Systemic Pathology

The place of high-tech methods of molecular biology in clinical laboratory diagnostics of various diseases and the development of a system of biomarkers as an important component of diagnostic research is currently attracting the closest attention of the scientific community. In this paper, an attempt is made to use high-tech metagenomic analysis to solve problems that arise due to the high frequency of association of periodontal diseases with systemic pathology, in particular, with type 2 diabetes mellitus. The aim of the study was to determine the taxonomic and metabolic features of the microbiome of periodontal tissues in periodontal diseases associated with type 2 diabetes mellitus, as a model of the ratio of local and systemic effects of periodontal pathogenic bacteria. The study included 16S shotgun sequencing of bacterial DNA as part of biological material from periodontal pockets/dentoalveolar furrows of 46 people - 15 patients with chronic periodontitis associated with type 2 diabetes mellitus, 15 patients with chronic periodontitis unrelated to systemic pathology, as well as 16 healthy people in the control group, followed by bioinformatic processing of the data obtained. The obtained data allowed us to establish the taxonomic features of the periodontal microbiome in the association of chronic periodontitis with type 2 diabetes mellitus, which included the predominance of representatives of the families Prevotellaceae and Spirochaetaceae in its composition. The features of metabolic processes in periodontal tissues with the participation of the microbiome were also revealed, which consisted in an increase in the exchange of cysteine and methionine against the background of a decrease in the metabolism of pyrimidine, methane, sphingolipids, and the synthesis of fatty acids, which are of diagnostic value in assessing the condition of patients with type 2 diabetes mellitus.

scholarly journals Application value of combination therapy of periodontal curettage and root planing on moderate-to-severe chronic periodontitis in patients with type 2 diabetes

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Yonghuan Bian ◽  
Changhao Liu ◽  
Zhaojiang Fu
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Blood Glucose ◽  
Chronic Periodontitis ◽  
Root Planing ◽  
Tnf Α ◽  
Significant Difference ◽  
Hs Crp

Abstract Background Our study attempted to observe the value of periodontal curettage combined with root planing on moderate-to-severe chronic periodontitis in patients with type 2 diabetes. Methods There involved 72 patients with type 2 diabetes mellitus complicated with moderate-to-severe chronic periodontitis who were diagnosed and treated in our hospital from January 2019 to December 2019. The patients enrolled were randomly divided into four groups using a computer-generated table: root planing and periodontal curettage combined group (n = 18), root planning group (n = 18), periodontal curettage group (n = 18) and cleansing group (n = 18). Blood glucose, plaque index (PI), gingival index (GI), probing depth (PD), attachment loss (AL), serum levels of inflammatory factors (Tumor Necrosis Factor Alpha [TNF- α] and hypersensitive C-reactive protein [hs-CRP]) were observed before and after treatment. The collecting dates were analyzed by the chi-square χ 2 test, repeated measurement analysis of variance, or t-test according to different data types and research objectives. Results Before treatment, there was no significant difference in PI, GI, PD and AL among the four groups (P> 0.05), while after 3-month treatment, the levels of PI, GI, PD and AL in the combined group were lower than those in the root planing group, periodontal curettage group and cleansing group, with both root planing group and periodontal curettage group significantly lower than cleansing group (P< 0.05). The fasting blood glucose, 2-h postprandial blood glucose and glycosylated hemoglobin in the combined group, root planing group, periodontal curettage group and cleansing group were significantly lower than those before treatment (P < 0.05). Before treatment, there was no significant difference in TNF- α and hs-CRP among the four groups (P> 0.05), but the levels of TNF- α and hs-CRP in the four groups decreased significantly after 3-month treatment (P< 0.05). The levels of TNF- α and hs-CRP in the combined group were lower than those in the root planing group, periodontal curettage group and cleansing group, and those in the root planing group and periodontal curettage group were significantly lower than those in the cleansing group (P< 0.05). Conclusion The combination therapy of periodontal curettage and root planing exerted beneficial effects on moderate-to-severe chronic periodontitis in patients with type 2 diabetes mellitus, which holds the potential to maintain the level of blood glucose and improve the quality of life of the patients.

Comparative evaluation of genotoxic effect in subjects with chronic periodontitis with and without type 2 diabetes mellitus

2019 ◽  
Vol 11 (1) ◽  
pp. 29-36
Author(s):  
Dr.Smruti Lulla ◽  
Dr.Amita Mali ◽  
Dr. AashwiinMiglani ◽  
Dr. VidyaDodwad ◽  
Dr. SachinMangalekar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Periodontal Diseases ◽  
Genotoxic Effect ◽  
Micronucleus Frequency ◽  
Group A

Aims: The aim of the study was to compare the genotoxic effect by assessing the micronucleus frequency (MNF), as a biomarker for DNA damage, in subjects with chronic periodontitis with and without type 2 Diabetes Mellitus. Methods and Material: For the study, a total of 65 subjects were take and divided into three groups. Group A included 30 subjects with Generalised Chronic Periodontitis, Group Bincluded 30 Subjects with Chronic Periodontitis with type 2 diabetes mellitus and Group C included 5 systemically and periodontally healthy subjects. Periodontal clinical examination was carried out. A slide was prepared using the blood sample collected from the subjects which was fixed in 5% geimsa solution and was observed under the microscope. Later the scoring of micronuclei was done. Statistical analysis used: Descriptive and inferential statistical analyses were carried out in the present study. Analysis of Variance (ANOVA) was used to find the significance of study parameters and test the equality of three or more means of more than two groups. Tukey’s post hoc analysis was used to compare parameters of each and every group. Results: The mean score of the micronuclei observed in group A, B and C were 10.23, 14.87 and 1 respectively i.e. group B showed significantly greater damage than other two groups. Conclusions: It was concluded that the score of micronucleus frequency may be considered as an important biomarker of genotoxic damage that is DNA damage in subjects with chronic localised as well as systemic diseases like type 2 diabetes, as well as Periodontal diseases.

MMP-2, MMP-8 Expression in gingival tissue of Chronic Periodontitis associated to Type 2 Diabetes Mellitus

2005 ◽  
Vol 35 (3) ◽  
pp. 661 ◽  
Author(s):  
Min Gu Kang ◽  
Hyun Jeong Cha ◽  
Sun Hee Song ◽  
Jin Woo Park ◽  
Jo Young Suh ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Chronic Periodontitis ◽  
Gingival Tissue
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