scholarly journals Composición y actividad antifúngica del aceite esencial de Fabiana imbricata contra levaduras del género Candida

Author(s):  
Alejandro Martín Madrid-Villegas ◽  
Camila Marcela Venegas-Valdés ◽  
Manuel Alejandro Martínez-Lobos ◽  
Ana Lizeth Morales-Abularach ◽  
Valeska Lorena Calderón-Fernández ◽  
...  

El objetivo de este estudio fue evaluar la actividad anti-fungica del aceite esencial (AE) de Fabiana imbricata Ruiz & Pav contra cinco especies de Candida comúnmente asociadas a enfermedades ginecológicas. El aceite esencial de las hojas fresca de F. imbricata fue obtenido por hidrodestilación y caracterizado mediante la técnica de cromatografía de gases acoplada a espectrometría de masas (CG/EM). Se evaluó la actividad anti-fúngica a diferentes concentraciones del AE mediante el método de microdilución. Los compuestos más abundantes identificados en el AE (7a-isopropenil-4,5-dimetiloctahidroinden-4-il) metanol (43,66%), bulnesol (17,02%) y T-muurolol (5,75%). El AE mostro una potente y selectiva actividad anti-fúngica sobre el crecimiento de la levadura patógena Candida guilliermondii con una concentración mínima inhibitoria de 10 μg/mL, superior al control positivo clotrimazol que presento un valor de 15 μg/mL. Estos resultados confirman las propiedades antimicrobianas de los aceites esenciales y su posible uso en el manejo de las cepas de C. guilliermondii de importancia para pacientes inmune deprimidos y como posibles alternativas a los fungicidas sintéticos.

1997 ◽  
Vol 43 (4) ◽  
pp. 362-367 ◽  
Author(s):  
M. J. R. Nout ◽  
C. E. Platis ◽  
D. T. Wicklow

Microflora in wound sites of preharvest maize (including bacteria, yeasts, and filamentous fungi) may play a role in attracting insects to maize plants and may also interact with growth and mycotoxin production by filamentous fungi. As little data are available about the yeasts occurring on maize from the U.S. corn belt, samples of milled maize from experimental plantings at the University of Illinois River Valley Sand Field were analyzed. Yeast counts showed slight yearly fluctuation and varied between 3.60 and 5.88 (log cfu/g maize). The majority of the yeasts were Candida guilliermondii (approximately 55%), Candida zeylanoides (24 %), Candida shehatae (11%), and Debaryomyces hansenii (3%). Also present were Trichosporon cutaneum, Cryptococcus albidus var. aerius, and Pichia membranifaciens. The occurrence of killer yeasts was also evaluated. Killer yeasts were detected in maize for the first time and were identified as Trichosporon cutaneum and Candida zeylanoides. These were able to kill some representative yeasts isolated from maize, including Candida guilliermondii, Candida shehatae, and Cryptococcus albidus var. aerius. Other maize yeasts (Candida zeylanoides, Debaryomyces hansenii, Pichia membranifaciens) were not affected. The majority of yeasts found on maize were unable to ferment its major sugars, i.e., sucrose and maltose. Some (e.g., Candida zeylanoides) were not even able to assimilate these sugars. The importance of these properties in relation to insect attraction to preharvest ears of maize is discussed.Key words: corn, maize, yeast, killer.


2009 ◽  
Vol 40 (3) ◽  
pp. 631-635 ◽  
Author(s):  
Ester Junko Tomotani ◽  
Priscila Vaz de Arruda ◽  
Michele Vitolo ◽  
Maria das Graças de Almeida Felipe

2000 ◽  
Vol 55 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
Walter de Carvalho ◽  
Silvio Silvério da Silva ◽  
Michele Vitolo ◽  
Ismael Maciel de Mancilha

Abstract In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrapment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125- and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest values of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric productivity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates.


2010 ◽  
Vol 26 (1) ◽  
pp. 27-30
Author(s):  
Alissa M Lockwood ◽  
Katleen N Wyatt ◽  
Prasad Abraham ◽  
Sean P Montgomery ◽  
Jana Macleod

2013 ◽  
Vol 58 (3) ◽  
pp. 1529-1537 ◽  
Author(s):  
Jesús Guinea ◽  
Óscar Zaragoza ◽  
Pilar Escribano ◽  
Estrella Martín-Mazuelos ◽  
Javier Pemán ◽  
...  

ABSTRACTWe report the molecular identifications and antifungal susceptibilities of the isolates causing fungemia collected in the CANDIPOP population-based study conducted in 29 Spanish hospitals. A total of 781 isolates (from 767 patients, 14 of them having mixed fungemia) were collected. The species found most frequently wereCandida albicans(44.6%),Candida parapsilosis(24.5%),Candida glabrata(13.2%),Candida tropicalis(7.6%),Candida krusei(1.9%),Candida guilliermondii(1.7%), andCandida lusitaniae(1.3%). OtherCandidaand non-Candidaspecies accounted for approximately 5% of the isolates. The presence of cryptic species was low. Compared to findings of previous studies conducted in Spain, the frequency ofC. glabratahas increased. Antifungal susceptibility testing was performed by using EUCAST and CLSI M27-A3 reference procedures; the two methods were comparable. The rate of fluconazole-susceptible isolates was 80%, which appears to be a decrease compared to findings of previous studies, explained mainly by the higher frequency ofC. glabrata. Using the species-specific breakpoints and epidemiological cutoff values, the rate of voriconazole and posaconazolein vitroresistance was low (<2%). In the case ofC. tropicalis, using the EUCAST procedure, the rate of azole resistance was around 20%. There was a correlation between the previous use of azoles and the presence of fluconazole-resistant isolates. Resistance to echinocandins was very rare (2%), and resistance to amphotericin B also was very uncommon. The sequencing of the hot spot (HS) regions fromFKS1orFKS2genes in echinocandin-resistant isolates revealed previously described point mutations. The decrease in the susceptibility to fluconazole in Spanish isolates should be closely monitored in future studies.


Author(s):  
Maria Antonieta P. Gimenes ◽  
Luiz Cláudio S. Carlos ◽  
Luís F. F. Faria ◽  
Nei Pereira

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