Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation

The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocrossing point, the wavelength 247 nm and 365 nm were selected for PYZ and RIF quantification, respectively. No interference from cubosome excipients was detected in the proposed method. The results demonstrated linearity in a range of 4.0 – 12.0 µg/mL with an adequate correlation coefficient for both drugs. The intra and inter-day precision results (RSD < 5%) indicated the reproducibility of the method. The accuracy data showed satisfactory results (RSD < 5%) from recovery test. In addition, the robustness results showed that the PYZ and RIF content were unaffected by the solvent alteration of methanol to methanol:water (99:1, v/v). The derivative ultraviolet spectrophotometric method proved to be an excellent strategy for simultaneous determination of PYZ and RIF.

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Simultaneous determination of benzocaine and cetylpiridinium chloride in tablets by first-derivative spectrophotometric method

Il Farmaco ◽

10.1016/s0014-827x(00)00092-6 ◽

2000 ◽

Vol 55 (11-12) ◽

pp. 687-693 ◽

Cited By ~ 11

Author(s):

L.R Paschoal ◽

W.A Ferreira

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

First Derivative

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Using a First-Derivative Spectrophotometric Method for Simultaneous Determination of Fe(II) and Ni(II) in Mineral Vitamins by Interaction with 2,4-Diamino-5-(2-hydroxy-5-nitrophenylazo)benzenesulfonic Acid Sodium Salt in the Presence of Tween 20

Journal of AOAC International ◽

10.1093/jaoac/93.3.833 ◽

2010 ◽

Vol 93 (3) ◽

pp. 833-841

Author(s):

Elham Y Hashem ◽

Mohamed M Seleim ◽

Ahmed M El-Zohry

Keyword(s):

Simultaneous Determination ◽

Sodium Salt ◽

Spectrophotometric Method ◽

Detection Limits ◽

Tween 20 ◽

Benzenesulfonic Acid ◽

First Derivative ◽

Binary Complexes ◽

Acid Sodium Salt

Abstract A highly selective and sensitive derivative spectrophotometric method has been developed for the determination of Fe(II) and Ni(II) in different mineral vitamins. The method is based on the formation of binary complexes of Fe(II) and Ni(II) with 2,4-diamino-5-(2-hydroxy-5-nitrophenylazo) benzenesulfonic acid sodium salt (Mordant Brown 33) at pH 5.6 with Tween 20. At max 512 and 493 nm, the molar absorbtivity was 2.09 104 and 0.58 104 L/mol cm for Fe(II) and Ni(II), respectively. Fe(II) can be determined in the range 0.552.79 g/mL in the presence of 2.92 g/mL Ni, and Ni(II) can be determined in the range 1.173.5 g/mL in the presence of 2.79 g/mL Fe(II) in the presence of Tween 20 (4). The detection limits were 9.0 and 15.0 ng/mL for Fe(II) and Ni(II), respectively. The effect of foreign ions was elucidated. The RSD values were, in all instances, less than 1.3. The proposed method was successfully applied for the simultaneous determination of Fe(II) and Ni(II) in different mineral vitamins.

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Simultaneous determination of enalapril maleate and hydrochlorothiazide by first-derivative ultraviolet spectrophotometry and high-performance liquid chromatography

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/0731-7085(95)01346-m ◽

1995 ◽

Vol 13 (7) ◽

pp. 851-856 ◽

Cited By ~ 50

Author(s):

Abdel Fattah M. El Walily ◽

Saeid F. Belal ◽

Eman A. Heaba ◽

Ali El Kersh

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Simultaneous Determination ◽

High Performance ◽

Ultraviolet Spectrophotometry ◽

Enalapril Maleate ◽

First Derivative

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Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph16071196 ◽

2019 ◽

Vol 16 (7) ◽

pp. 1196 ◽

Cited By ~ 6

Author(s):

Mahesh Attimarad ◽

Muhammad Shahzad Chohan ◽

Abdulmalek Ahmed Balgoname

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

High Performance ◽

Internal Standard ◽

Reversed Phase ◽

Simultaneous Estimation ◽

Zero Crossing ◽

Spectrophotometric Methods ◽

First Derivative

Simple, fast, and precise reversed-phase (RP)-high-performance liquid chromatography (HPLC) and two ecofriendly spectrophotometric methods were established and validated for the simultaneous determination of moxifloxacin HCl (MOX) and flavoxate HCl (FLX) in formulations. Chromatographic methods involve the separation of two analytes using an Agilent Zorbax SB C18 HPLC column (150 mm × 4.6 mm; 5 µm) and a mobile phase consisting of phosphate buffer (50 mM; pH 5): methanol: acetonitrile in a proportion of 50:20:30 v/v, respectively. Valsartan was used as an internal standard. Analytes were monitored by measuring the absorbance of elute at 299 nm for MOX and 250 nm for FLX and valsartan. Two environmentally friendly spectrophotometric (first derivative and ratio first derivative) methods were also developed using water as a solvent. For the derivative spectrophotometric determination of MOX and FLX, a zero-crossing technique was adopted. The wavelengths selected for MOX and FLX were −304.0 nm and −331.8 nm for the first derivative spectrophotometric method and 358.4 nm and −334.1 nm for the ratio first-derivative spectrophotometric method, respectively. All methods were successfully validated, as per the International Conference on Harmonization(ICH) guidelines, and all parameters were well within acceptable ranges. The proposed analytical methods were successfully utilized for the simultaneous estimation of MOX and FLX in formulations.

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Simultaneous determination of cinnarizine and nicergoline in a binary mixture using first derivative spectra, first derivative of ratio spectra and multivariate calibration techniques

Journal of Analytical Chemistry ◽

10.1134/s1061934810090066 ◽

2010 ◽

Vol 65 (9) ◽

pp. 921-928

Author(s):

S. I. M. Zayed

Keyword(s):

Binary Mixture ◽

Simultaneous Determination ◽

Multivariate Calibration ◽

First Derivative ◽

Derivative Spectra ◽

Calibration Techniques

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Simultaneous Determination Of Atenolol And Hydrocholrothiazide In Tablets Formulation By Derivative Spectrometry

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v17i.8808 ◽

2020 ◽

Vol 17 ◽

pp. 73-79

Author(s):

SAAD ANTAKLI ◽

LEON NEJEM ◽

MOUSTAFA ALABO JOUMAA

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

Correlation Coefficients ◽

First Derivative ◽

Derivative Spectrometry

The derivative spectrophotometric method was developed and applied for the simultaneous determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in Tablets formulations. The first derivative spectrophotometric (1DS) method was applied for the determination of (ATE) and (HCT), respectively. (ATE) was determined at 271.9 nm (1D 271.9) and (HCT) was determined at 279.3 nm (1D 279.3). Linearity showed a good correlation coefficients R2 = 0.9994 and R2 = 0.9989 for (ATE) and (HCT), respectively. Linearity ranges were (10 – 280)

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APPLICATION OF RATIO DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ARTEMISININ AND CURCUMIN

INDIAN DRUGS ◽

10.53879/id.52.08.10316 ◽

2015 ◽

Vol 52 (08) ◽

pp. 43-47

Author(s):

R Dhankecha ◽

◽

A Soni ◽

M Gohel ◽

V Thakkar ◽

...

Keyword(s):

Simultaneous Determination ◽

Absorption Spectra ◽

Spectrophotometric Method ◽

Dosage Form ◽

Simultaneous Estimation ◽

Direct Absorption ◽

Acceptable Limit ◽

First Derivative ◽

Ich Guidelines

Ratio derivative spectrophotometric method has been developed for the simultaneous determination of artemisinin and curcumin. The overlapping spectra of both drugs were resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of artemisinin and curcumin were measured at λmax 293.17 and λmax 457.0 nm, respectively, for their quantification. Artemisinin and curcumin were determined in the concentration range of 2-10 μg/mL and 1-5 μg/mL, respectively. The method was validated as per the ICH guidelines and accuracy, precision were found to be within acceptable limit. The limits of detection and quantitation were found to be 0.003299 and 0.009997 μg/mL, respectively for artemisinin and 0.006743 and 0.020434 μg/mL, respectively for curcumin. The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and successfully applicable for simultaneous estimation of artemisinin and curcumin in parentral dosage form.

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