scholarly journals Simultaneous Determination Of Atenolol And Hydrocholrothiazide In Tablets Formulation By Derivative Spectrometry

2020 ◽  
Vol 17 ◽  
pp. 73-79
Author(s):  
SAAD ANTAKLI ◽  
LEON NEJEM ◽  
MOUSTAFA ALABO JOUMAA
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Correlation Coefficients ◽  
First Derivative ◽  
Derivative Spectrometry

The derivative spectrophotometric method was developed and applied for the simultaneous determination of Atenolol (ATE) and Hydrochlorothiazide (HCT) in Tablets formulations. The first derivative spectrophotometric (1DS) method was applied for the determination of (ATE) and (HCT), respectively. (ATE) was determined at 271.9 nm (1D 271.9) and (HCT) was determined at 279.3 nm (1D 279.3). Linearity showed a good correlation coefficients R2 = 0.9994 and R2 = 0.9989 for (ATE) and (HCT), respectively. Linearity ranges were (10 – 280)

scholarly journals Simultaneous determination of procaine hydrochloride, procainamide hydrochloride and lidocaine by molecular absorption spectrometry

2019 ◽  
pp. 318-324
Author(s):  
Nicoleta Mirela Marin ◽  
Gheorghe Batrinescu ◽  
Mihai Nita-Lazar ◽  
Luoana Florentina Pascu ◽  
Carol Blaziu Lehr
Keyword(s):  
Simultaneous Determination ◽  
Direct Method ◽  
Absorption Spectrometry ◽  
Procaine Hydrochloride ◽  
Zero Crossing ◽  
Multicomponent Analysis ◽  
First Derivative ◽  
Cramer’S Rule ◽  
Derivative Spectrometry

Two spectrometric methods have been developed for quantitative simultaneous determination of procaine hydrochloride (PH·HCl), procainamide hydrochloride (PHA·HCl) and lidocaine (Lid) from synthetic mixture. The methods employed are first derivative spectrometry, using zero crossing method and multicomponent analysis which is based on the additivity law. Using first derivative spectrometry, the wavelength selected for the quantitative determination of PH·HCl was 237 nm for Lid was 242 nm and for PHA·HCl was 290 nm in mixture. The method is linear when the concentration ranged between 6.62-9.93 μg/mL for PH·HCl, 6.43-9.64 for PHA·HCl and 5.56-8.35 for Lid. The multicomponent analysis is a direct method and involves the absorbance measurements of at three different wavelengths. The molar absorption coefficients values were calculated at each wavelength and the concentration of PH·HCl, PHA·HCl and Lid from mixture was determined by solving matrix using Cramer's rule. The recovery of each compound in mixture was calculated and it is 101.4 % for PH·HCl, 100.4 % for PHA·HCl and 98.4 % for Lid.

scholarly journals Using a First-Derivative Spectrophotometric Method for Simultaneous Determination of Fe(II) and Ni(II) in Mineral Vitamins by Interaction with 2,4-Diamino-5-(2-hydroxy-5-nitrophenylazo)benzenesulfonic Acid Sodium Salt in the Presence of Tween 20

2010 ◽  
Vol 93 (3) ◽  
pp. 833-841
Author(s):  
Elham Y Hashem ◽  
Mohamed M Seleim ◽  
Ahmed M El-Zohry
Keyword(s):  
Simultaneous Determination ◽  
Sodium Salt ◽  
Spectrophotometric Method ◽  
Detection Limits ◽  
Tween 20 ◽  
Benzenesulfonic Acid ◽  
First Derivative ◽  
Binary Complexes ◽  
Acid Sodium Salt

Abstract A highly selective and sensitive derivative spectrophotometric method has been developed for the determination of Fe(II) and Ni(II) in different mineral vitamins. The method is based on the formation of binary complexes of Fe(II) and Ni(II) with 2,4-diamino-5-(2-hydroxy-5-nitrophenylazo) benzenesulfonic acid sodium salt (Mordant Brown 33) at pH 5.6 with Tween 20. At max 512 and 493 nm, the molar absorbtivity was 2.09 104 and 0.58 104 L/mol cm for Fe(II) and Ni(II), respectively. Fe(II) can be determined in the range 0.552.79 g/mL in the presence of 2.92 g/mL Ni, and Ni(II) can be determined in the range 1.173.5 g/mL in the presence of 2.79 g/mL Fe(II) in the presence of Tween 20 (4). The detection limits were 9.0 and 15.0 ng/mL for Fe(II) and Ni(II), respectively. The effect of foreign ions was elucidated. The RSD values were, in all instances, less than 1.3. The proposed method was successfully applied for the simultaneous determination of Fe(II) and Ni(II) in different mineral vitamins.

scholarly journals Analytical Spectrometric Study For Determining Dapagliflozin Propanediol Monohydrate Individually Or In Presence Of Metformin Hydrochloride In Tablets Formulation

2020 ◽  
Vol 17 ◽  
pp. 80-87
Author(s):  
SAAD ANTAKLI ◽  
Raghad Kabbani ◽  
And Rama Labban
Keyword(s):  
Regression Analysis ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Metformin Hydrochloride ◽  
Spectrometric Study ◽  
Limit Of Quantification ◽  
First Derivative ◽  
Linearity Range

First simple spectrophotometric method was developed and applied to determine Dapagliflozin Propanediol  Monohydrate by Zero Spectrophotometry and First Derivative Spectrophotometric method for determining of Dapagliflozin Propanediol Monohydrate (DAPA) in the presence of Metformin Hydrochloride (MET). Zero spectrophotometric (ZS) was applied for the determination of (DAPA) at 223.5 nm. Linearity range was (2.61– 31.23) µg/mL. Regression analysis showed a good correlation coefficients R2 = 0.9989. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.569 µg/mL and 1.724 µg/mL, respectively. Derivative spectrophotometric (1DS) was applied for the determination of (DAPA) in the presence (MET). (DAPA) was determined at 233 nm (1D233).  Linearity ranges were (5.21 – 41.64) µg/mL for (DAPA). Regression analysis showed a good correlation coefficients R2 = 0.9994. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.732 µg/mL and 2.218 µg/mL for (DAPA). The proposed Zero spectrophotometry method was applied to analysis individual (DAPA), and the derivative (1D233) method was applied to analysis (DAPA) individually or with (MET) combination in Syrian trademark drugs.

scholarly journals Simultaneous Determination of Moxifloxacin and Flavoxate by RP-HPLC and Ecofriendly Derivative Spectrophotometry Methods in Formulations

2019 ◽  
Vol 16 (7) ◽  
pp. 1196 ◽  
Author(s):  
Mahesh Attimarad ◽  
Muhammad Shahzad Chohan ◽  
Abdulmalek Ahmed Balgoname
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
High Performance ◽  
Internal Standard ◽  
Reversed Phase ◽  
Simultaneous Estimation ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
First Derivative

Simple, fast, and precise reversed-phase (RP)-high-performance liquid chromatography (HPLC) and two ecofriendly spectrophotometric methods were established and validated for the simultaneous determination of moxifloxacin HCl (MOX) and flavoxate HCl (FLX) in formulations. Chromatographic methods involve the separation of two analytes using an Agilent Zorbax SB C18 HPLC column (150 mm × 4.6 mm; 5 µm) and a mobile phase consisting of phosphate buffer (50 mM; pH 5): methanol: acetonitrile in a proportion of 50:20:30 v/v, respectively. Valsartan was used as an internal standard. Analytes were monitored by measuring the absorbance of elute at 299 nm for MOX and 250 nm for FLX and valsartan. Two environmentally friendly spectrophotometric (first derivative and ratio first derivative) methods were also developed using water as a solvent. For the derivative spectrophotometric determination of MOX and FLX, a zero-crossing technique was adopted. The wavelengths selected for MOX and FLX were −304.0 nm and −331.8 nm for the first derivative spectrophotometric method and 358.4 nm and −334.1 nm for the ratio first-derivative spectrophotometric method, respectively. All methods were successfully validated, as per the International Conference on Harmonization(ICH) guidelines, and all parameters were well within acceptable ranges. The proposed analytical methods were successfully utilized for the simultaneous estimation of MOX and FLX in formulations.

scholarly journals Determination of Glibenclamide By Analytical Spectrophotometry

2021 ◽  
Vol 18 ◽  
pp. 40-48
Author(s):  
Saad Antakli ◽  
Leon Nejem ◽  
Monzer Alraii
Keyword(s):  
Quality Control ◽  
Regression Analysis ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Extraction Process ◽  
Metformin Hydrochloride ◽  
Limit Of Quantification ◽  
First Derivative

Simple and rapid spectrophotometric method was developed and applied to determine Glibenclamide (GB) by zero spectrophotometric method and first derivative spectrophotometric method for determining of (GB) in the presence of Metformin hydrochloride (MET). Zero spectrophotometric (ZS) method was applied for the determination of (GB) at λmax = 300 nm. Linearity range was (4 – 360) μg/mL. Regression analysis showed a good correlation coefficients R2 = 0.99993. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.65 μg/mL and 2.31 μg/mL, respectively.  First derivative spectrophotometric (1DS) method was applied for the determination of (GB) in the presence (MET). (GB) was determined at 317 nm (1D317). Linearity ranges were (4 – 240) μg/mL for (GB). Regression analysis showed a good correlation coefficients R2 = 0.999914. The limit of detection (LOD) and limit of quantification (LOQ) were to be 0.60 μg/mL and 1.83 μg/mL for (GB). The proposed zero spectrophotometry method was applied to analysis individual (GB), and the derivative (1D317) method was applied to analysis (GB) individually or combined with (MET) in Syrian trademark drugs. The proposed method is simple, direct, sensitive and do not require any extraction process. Thus, this method could be readily applicable for the quality control and routine analysis.

scholarly journals Derivative spectrophotometric determination of the herbicides picloram and triclopyr in mixtures

2007 ◽  
Vol 72 (8-9) ◽  
pp. 809-819 ◽  
Author(s):  
Biljana Abramovic ◽  
Vesna Anderluh ◽  
Ferenc Gaál ◽  
Daniela Sojic
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Correlation Coefficients ◽  
Second Derivative ◽  
Deconvolution Method ◽  
First Derivative ◽  
Pesticide Formulations ◽  
Influence Of Ph

A derivative spectrophotometric method for the determination of the herbicides picloram (4-amino-3,5,6-trichloropicolinic acid) and triclopyr (3,5,6-trichloro- 2-pyridyloxyacetic acid) in mixtures was developed in this work. Derivative spectrophotometric determination of the selected herbicides was preceded by investigations concerning the influence of pH, in the pH interval from 1 to 9. At pH 3.2, picloram and triclopyr solutions are stable under daylight for nine months. This pH was used for all subsequent determinations. It was also found that the use of the first derivative of the spectra at 232 nm was optimal for the determination of picloram, while use of the second derivative of the spectra at 211 nm was best suited for the determination of triclopyr. The calibration curves are linear in the concentration range 0.8 - 13 ?g cm-3 with correlation coefficients -0.9998 for picloram and 0.9996 for triclopyr. The limit of detection of the developed method is 0.08 ?g cm-3 for picloram and 0.03 ?g cm-3 for triclopyr. Derivative spectrophotometry was shown to be an appropriate method for the determination of picloram and triclopyr in mixtures and in pesticide formulations, unlike the deconvolution method. .

APPLICATION OF RATIO DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ARTEMISININ AND CURCUMIN

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 43-47
Author(s):  
R Dhankecha ◽  
◽  
A Soni ◽  
M Gohel ◽  
V Thakkar ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Absorption Spectra ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Direct Absorption ◽  
Acceptable Limit ◽  
First Derivative ◽  
Ich Guidelines

Ratio derivative spectrophotometric method has been developed for the simultaneous determination of artemisinin and curcumin. The overlapping spectra of both drugs were resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of artemisinin and curcumin were measured at λmax 293.17 and λmax 457.0 nm, respectively, for their quantification. Artemisinin and curcumin were determined in the concentration range of 2-10 μg/mL and 1-5 μg/mL, respectively. The method was validated as per the ICH guidelines and accuracy, precision were found to be within acceptable limit. The limits of detection and quantitation were found to be 0.003299 and 0.009997 μg/mL, respectively for artemisinin and 0.006743 and 0.020434 μg/mL, respectively for curcumin. The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and successfully applicable for simultaneous estimation of artemisinin and curcumin in parentral dosage form.

scholarly journals Development of derivative spectrophotometric method for simultaneous determination of pyrazinamide and rifampicin in cubosome formulation

2021 ◽  
Vol 5 (1) ◽  
pp. 46-50
Author(s):  
Morgana Souza Marques ◽  
Fernando Dal Pont Morisso ◽  
Fernanda Poletto ◽  
Irene Clemes Külkamp Guerreiro
Keyword(s):  
Simultaneous Determination ◽  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Ultraviolet Spectrophotometry ◽  
Recovery Test ◽  
First Derivative ◽  
Derivative Spectra ◽  
Accuracy Data ◽  
Detection And Quantification

The ultraviolet spectrophotometry analysis for quantitative assay of drugs is a method accurate, sensitive, selective and reproductive with the advantage of being a simple and less expensive method. In this study, a derivative ultraviolet spectrophotometric method was developed for simultaneous determination of pyrazinamide (PYZ) and rifampicin (RIF). The spectrophotometric method was evaluated according to validation guidelines for specificity, linearity, limits of detection and quantification, precision, accuracy and robustness. The first-derivative spectra were obtained and by the zerocrossing point, the wavelength 247 nm and 365 nm were selected for PYZ and RIF quantification, respectively. No interference from cubosome excipients was detected in the proposed method. The results demonstrated linearity in a range of 4.0 – 12.0 µg/mL with an adequate correlation coefficient for both drugs. The intra and inter-day precision results (RSD < 5%) indicated the reproducibility of the method. The accuracy data showed satisfactory results (RSD < 5%) from recovery test. In addition, the robustness results showed that the PYZ and RIF content were unaffected by the solvent alteration of methanol to methanol:water (99:1, v/v). The derivative ultraviolet spectrophotometric method proved to be an excellent strategy for simultaneous determination of PYZ and RIF.

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