scholarly journals Development of an in vitro Callus Induction Protocol and Shoot Proliferation for Selected Jatropha (Jatropha curcas) Accessions

Authorea ◽  
2020 ◽  
Author(s):  
Hundessa Gudeta ◽  
Meseret Tesema ◽  
Jiregna Daksa ◽  
Temesgen Magule
Keyword(s):  
Callus Induction ◽  
Jatropha Curcas ◽  
Shoot Proliferation

scholarly journals Development of an in vitro Callus Induction Protocol and Shoot Proliferation for Selected Jatropha (Jatropha curcas) Accessions

2020 ◽  
Vol 30 (1) ◽  
pp. 131-141
Author(s):  
Hundessa Fufa ◽  
Jiregna Daksa
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Jatropha Curcas ◽  
Plant Tissue ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Regeneration Medium ◽  
Adventitious Shoot Buds

The present study was undertaken to establish a protocol for in vitro callusing of three Jatropha accessions, namely Metema, Adami Tulu and Shewa Robit from leaf explants. The medium supplemented with combination of 4.44 μM BAP and 4.52 μM 2,4-D resulted in maximum percentage of callus (100%) formed for all accessions. The maximum shoot regeneration (66.67%) from callus with 10.13 number of shoot was obtained from Shewa Robit in MS medum fortified with TDZ (2.27 μM ) and IBA (0.49 μM ). The presence of TDZ in the shoot regeneration medium has greater influence on the induction of adventitious shoot buds, whereas MS supplemented with BAP alone and combination with IBA did not induce shoot regeneration from callus culture. The results obtained in the present study would facilitate the high callus induction and regeneration responses in Jatropha for its improvement using biotechnological tools. Plant Tissue Cult. & Biotech. 30(1): 131-141, 2020 (June)

scholarly journals In vitro propagation of six selected sugarcane mutant clones through leaf explants

2021 ◽  
Vol 883 (1) ◽  
pp. 012075
Author(s):  
R Purnamaningsih ◽  
D Sukmadjaja ◽  
S Suhesti ◽  
S Rahayu
Keyword(s):  
Callus Induction ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Culture Techniques ◽  
High Productivity ◽  
Media Formulation ◽  
Number Of Shoots

Abstract Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

scholarly journals Evaluation of In Vitro Germination and Callus Induction for Antimicrobial Activities of Jatropha curcas L.

2018 ◽  
Vol 4 (2) ◽  
pp. 104-124
Author(s):  
Manal Soliman ◽  
Khallil Elhalafawi ◽  
Ibrahim Ibrahim ◽  
Emad Mahrous ◽  
Metwally Bekhit
Keyword(s):  
Callus Induction ◽  
Jatropha Curcas ◽  
Antimicrobial Activities ◽  
In Vitro Germination ◽  
Jatropha Curcas L

EFFECTS OF BAP CONCENTRATION ON IN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION OF JATROPHA CURCAS

2011 ◽  
pp. 203-208 ◽  
Author(s):  
A. Shamsiah ◽  
A. Awal ◽  
S. Nurathrah ◽  
M. Khairul-Azmir ◽  
J.S. Norrizah
Keyword(s):  
Callus Induction ◽  
Jatropha Curcas ◽  
Shoot Multiplication

scholarly journals In vitro Callus induction and Shoot proliferation in Jojoba Simmondsia chinesis (Link) Schn.

2008 ◽  
Vol 2 (1) ◽  
pp. 33-41
Author(s):  
Nabeel K. Al-ِAni ◽  
Ashwaq S. Abd ◽  
Kadhim M. Ibrahim ◽  
Arwa A. Tawfiq
Keyword(s):  
Callus Induction ◽  
Vegetative Growth ◽  
Shoot Growth ◽  
Shoot Proliferation ◽  
Vegetative Shoot

From economic site;jojoba Simmondsia chinesis (Link) Schn. is very important shrub as its seeds contain high percent of oily wax . This wax composes natural and chemical charachteristics which makes shrub to be involved in many trade, manufacture and medical uses. In this study several interactions between auxins and cytokinins and gibbrellin were implemented to find out the best concentration for callus induction, vegetative growth and differentiation for experiments. BA at(2 or 5) mg/l, NAA at (0.018, 2, 2, 5) mg/l and GA3 at (0.1 or 0.2) mg/l were used. Results showed that highest percent for callus induction were (50 and 70) % respectively in the treatment (2) mg/l BA and (2) mg/l for both BA and NAA. The results revealed that using Kin. plus NAA with (0.1 or 0.2) mg/l GA3, was the best concentration lead to high percent of differentiation for vegetative shoot were (40 and 44) % respectively However, the length of vegetative shoots were (2 and 2.5) cm respectively. , this indicates the importance of the balance between these phytohormones required for shoot growth.

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

scholarly journals In vitro Regeneration of Vitex negundo L. - A Multipurpose Woody Aromatic Medicinal Shrub

1970 ◽  
Vol 18 (1) ◽  
pp. 37-42 ◽  
Author(s):  
M. Jawahar ◽  
S. Ravipaul ◽  
M. Jeyaseelan
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Optimal Level ◽  
Vitex Negundo ◽  
Indirect Organogenesis ◽  
Shoot Bud ◽  
Bud Initiation

A rapid and efficient protocol was developed for inducing indirect organogenesis using leaf explants of Vitex negundo L. Explants were cultured on MS with different concentrations of 2,4-D and IAA in combination with BAP for callus induction. The frequency of callus induction increased with increasing concentration of IAA (0.3 mg/l) and BAP (0.3 mg/l) at optimal level. The shoot buds appeared emerging as green coloured protuberances on the callus. The high frequency of shoot bud initiation and shoot proliferation was observed on MS containing 0.3 mg/l IAA and 0.3 mg/l BAP. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l IBA. Rooted plants were transferred to pots containing sand, soil and manure in the ratio of 1 : 1 : 1. Nearly 90% survival of in vitro plants were recorded. Key words : Vitex negundo, In vitro, Leaf, Callus, Regeneration D.O.I. 10.3329/ptcb.v18i1.3263 Plant Tissue Cult. & Biotech. 18(1): 37-42, 2008 (June)

scholarly journals Perbanyakan Anggrek Spesies Paphiopedilum glaucophyllum J.J.Smith melalui Proliferasi Tunas Adventif Secara In Vitro

2015 ◽  
Vol 4 (3) ◽  
pp. 115
Author(s):  
Tubagus Kiki Kawakibi Azmi ◽  
Ni Made Armini Wiendi
Keyword(s):  
Seed Germination ◽  
Root Growth ◽  
Factorial Design ◽  
Key Words ◽  
Callus Induction ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Ms Medium ◽  
Optimum Growth

<p>ABSTRACT</p><p>This research was  aimed  to determine the effects of  BAP and medium on adventitious  shoot proliferation of  Paphiopedilum  glaucophyllum  J.J.  Smith in vitro. Plantlet of 1 year 9 months old from  seed  germination  in  vitro  on modified  Knudson  C  medium  was  used  as  an  explant.  This research was arranged in a Factorial Design with three  replications. The first factor was different concentration of BAP, consisting  of 1 and 2 mg L-1(All combination medium were added with 0.5 mg L-12.4-D). The second factor was different concentration of macro and micro nutrient from MS (Murashige and Skoog) and KC (Knudson C) medium, consisting  of 1, ¾, ½, and ¼ concentrationsof macro and  micro  nutrient.  The  result  showed  that  plantlets  failed  to  response to  the  entireadventitious  shoot  proliferation  combination  medium  within 16 weeks.  Concentration  of  medium affected to  leaves and root growth. The optimum growth was achieved at ¾ concentration of macro and micro nutrient from KC medium, combined with 2 mg L-1BAP for leaves growth and 1 mg L-1BAP for root growth. BAP affected callus  induction. The highest percentage of callus induction  was achieved at 1 mg L-1BAP (48.61%).</p><p>Key words: BAP, KC, MS, medium, proliferation.</p><p> </p><p>ABSTRAK</p><p>Penelitian dilakukan untuk mempelajari pengaruh zat pengatur tumbuh BAP dan media MS (Murashige  &amp;  Skoog)  dan  KC  (Knudson  C)  terhadap kemampuan proliferasi  tunas  adventif Paphiopedilum  glaucophyllum  J.J.Smith  secara  in vitro.  Bahan  tanaman  yang  digunakan  adalah planlet hasil pengecambahan biji secara in vitro  yang telah berumur 1 tahun 9 bulan yang  diperoleh dari Pusat Konservasi Tumbuhan,  Kebun Raya,  Bogor.  Penelitian  menggunakan Rancangan Acak Lengkap  (RAL)  dengan  dua  faktor  perlakuan  yang  disusun secara  faktorial  dengan  tiga  ulangan. Faktor  pertama  adalah  BAP  yang terdiri  dari  1  dan  2  mg  L-1,  penggunaan  BAP  dikombinasikan dengan 0.5 mg L-1 2.4-D. Faktor kedua adalah media (MS dan KC) dengan konsentrasi hara makro dan mikro masing-masing adalah 1, ¾, ½, dan ¼ konsentrasi. Hasil penelitian menunjukkan bahwa proliferasi  tunas  adventif  tidak  terjadi pada  semua  perlakuan.  Konsentrasi  media  (MS  dan  KC) berpengaruh nyata terhadap  pertumbuhan  daun  dan  akar.  Pertumbuhan  daun  dan  akar optimum diperoleh  pada  ¾  konsentrasi  hara  makro  dan  mikro  pada kedua jenis  media.  Pertumbuhan  daun tertinggi diperoleh pada media KC ¾ konsentrasi hara makro dan mikro ditambah 2 mg L-1. Media KC  ¾ konsentrasi hara  makro  dan  mikro  ditambah  1  mg  L-1menghasilkan  jumlah  total akar tertinggi.  BAP  berpengaruh  nyata  terhadap  persentase  planlet berkalus.  Jumlah  planlet  berkalus tertinggi diperoleh pada media yang mengandung 1 mg L-1 BAP (48.61%).</p><p>Kata kunci: BAP, KC, MS, media, proliferasi.</p>

scholarly journals In vitro mass multiplication of Jatropha (Jatropha curcas L.) through axillary bud culture

2014 ◽  
Vol 6 (1) ◽  
pp. 189-192 ◽  
Author(s):  
L.K. Behera ◽  
M. R. Nayak ◽  
D. Nayak ◽  
D.B. Jadeja
Keyword(s):  
Jatropha Curcas ◽  
Shoot Proliferation ◽  
Axillary Bud ◽  
Nodal Segment ◽  
Ms Medium ◽  
Root Initiation ◽  
Jatropha Curcas L ◽  
Mass Multiplication ◽  
Axillary Bud Culture

The present investigation was conducted for mass multiplication of Jatropha curcas L. through axillary bud culture. For this nodal segment from 3-5 months old nursery grown plants were used as explants for axillary bud culture. The sterilization treatment involving dipping explants in 0.1 per cent HgCl2 solution for 5 minutes resulted in minimum contamination and maximum establishment of nodal explants. The treatment MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA was the best for culture establishment, shoot proliferation and multiplication of the axillary buds which exhibited highest value in each parameter like establishment (76.1%), number of days taken for shoot initiation (3.1 days), length of longest shoot (6.8 cm), number of leaves on main shoot (7.1) and number of shoots per explant (6.3). Among different treatments for root initiation, half MS media fortified with 1 mg/L IBA, 3 mg/ L NAA and 0.25 g AC gave best result in maximum number of rooting percentage (60) with minimum time taken for root initiation (13.3 days), produced maximum number of roots per shoots (5.1) and length of longest root (4.9 cm) when established shoots were treated with it. Such produced plantlets showed nearly cent per cent survival after hardening and acclimatization. It showed that explants surface sterilized with 0.1 per cent HgCl2 solution for 5 minutes inoculated in MS medium supplemented with 1.0 mg/L BAP and 1.0 mg/L IAA and half MS media fortified with 1 mg/L IBA, 3 mg/L NAA and 0.25 g AC were best in shoot establishment and root development respectively for mass multiplication of J. curcas L. through axillary bud culture.

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