Application of Microfluidic Technology in Field of Antibody Preparation

Microfluidic technology is a science and technology that can accurately manipulate fluids in micro-sized channels. In recent years, microfluidic devices have attracted wide attention due to its easy manipulation, miniaturized size, high throughput and precise control, which provide a potential platform for antibody screening. This review paper provides an overview of recent advances in microfluidic methods application in the field of antibody preparation. While hybridoma technology and four antibody engineering techniques including phage display, single B cell antibody screening, antibody expression and cell-free protein synthesis are mainly introduced, important advances of experimental models and results are also discussed. Furthermore, the authors expound on the limitations of current microfluidic screening system and present future directions of antibody screening platform based on microfluidics. Antibody preparation on microfluidics combined with other technologies has huge application potential in the field of biomedicine, and it is anticipated to be further developed.

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A Comparison of Autoanalyzer and Manual Methods for Red Cell Antibody Screening

Transfusion ◽

10.1111/j.1537-2995.1974.tb04583.x ◽

1974 ◽

Vol 14 (6) ◽

pp. 586-589

Author(s):

R. T. Morehead ◽

K. Anderson ◽

S. Grunewald ◽

D. Casey ◽

J. Mccullough

Keyword(s):

Red Cell ◽

Antibody Screening ◽

Cell Antibody ◽

Manual Methods

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Polybrene technique for red cell antibody screening using microplates.

Journal of Clinical Pathology ◽

10.1136/jcp.41.5.556 ◽

1988 ◽

Vol 41 (5) ◽

pp. 556-557 ◽

Cited By ~ 8

Author(s):

J A Lown ◽

J G Ivey

Keyword(s):

Red Cell ◽

Antibody Screening ◽

Cell Antibody

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Detection of antibodies to co-trimoxazole (preservative drug) interfering with routine red cell antibody screening

Asian Journal of Transfusion Science ◽

10.4103/ajts.ajts_119_16 ◽

2018 ◽

Vol 12 (1) ◽

pp. 89 ◽

Cited By ~ 1

Author(s):

Deepti Sachan ◽

SAswin Kumar

Keyword(s):

Red Cell ◽

Antibody Screening ◽

Cell Antibody

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Red Cell Antibody Screening with the Groupamatic System

Vox Sanguinis ◽

10.1159/000466883 ◽

1979 ◽

Vol 37 (1) ◽

pp. 55-61

Author(s):

M. Garretta ◽

J. Gener ◽

I. de Jerphanion ◽

D. Jacquet ◽

A. Muller

Keyword(s):

Red Cell ◽

Antibody Screening ◽

Cell Antibody

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Advancements and Potential Applications of Microfluidic Approaches—A Review

Chemosensors ◽

10.3390/chemosensors6040046 ◽

2018 ◽

Vol 6 (4) ◽

pp. 46 ◽

Cited By ~ 6

Author(s):

Ishtiaq Ahmed ◽

Zain Akram ◽

Mohammed Bule ◽

Hafiz Iqbal

Keyword(s):

Modern Science ◽

Cellular Interactions ◽

Cellular Microenvironment ◽

Engineering Research ◽

Precise Control ◽

Microfluidic Technology ◽

Potential Applications ◽

Micro Level

A micro-level technique so-called “microfluidic technology or simply microfluidic” has gained a special place as a powerful tool in bioengineering and biomedical engineering research due to its core advantages in modern science and engineering. Microfluidic technology has played a substantial role in numerous applications with special reference to bioscience, biomedical and biotechnological research. It has facilitated noteworthy development in various sectors of bio-research and upsurges the efficacy of research at the molecular level, in recent years. Microfluidic technology can manipulate sample volumes with precise control outside cellular microenvironment, at micro-level. Thus, enable the reduction of discrepancies between in vivo and in vitro environments and reduce the overall reaction time and cost. In this review, we discuss various integrations of microfluidic technologies into biotechnology and its paradigmatic significance in bio-research, supporting mechanical and chemical in vitro cellular microenvironment. Furthermore, specific innovations related to the application of microfluidics to advance microbial life, solitary and co-cultures along with a multiple-type cell culturing, cellular communications, cellular interactions, and population dynamics are also discussed.

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Red cell antibody screening in South India: lessons learnt

ISBT Science Series ◽

10.1111/voxs.12174 ◽

2015 ◽

Vol 10 (1) ◽

pp. 41-44

Author(s):

K. Ambuja ◽

C. Shivaram

Keyword(s):

South India ◽

Red Cell ◽

Antibody Screening ◽

Lessons Learnt ◽

Cell Antibody

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Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Journal of Blood Transfusion ◽

10.1155/2014/532919 ◽

2014 ◽

Vol 2014 ◽

pp. 1-5 ◽

Cited By ~ 9

Author(s):

Dietmar Enko ◽

Claudia Habres ◽

Franz Wallner ◽

Barbara Mayr ◽

Gabriele Halwachs-Baumann

Keyword(s):

Blood Cell ◽

Red Blood Cell ◽

Blood Samples ◽

Antibody Screening ◽

Indirect Antiglobulin Test ◽

Cell Antibody ◽

Antiglobulin Test ◽

Low Ionic Strength ◽

Enzyme Test ◽

Screening Result

The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n=98) of all patients (n=2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

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