Carotid intima-media thickness, 25-OH vitamin D, homocysteine and subclinical coronary artery atherosclerosis in patients with type 1 diabetes mellitus

Abstract Background We investigated the role of ANGPTL3 and ANGPTL4 in atherosclerosis development and determined whether plasma concentrations of ANGPTL3 and ANGPTL4 are related to the degree of coronary stenosis. Methods A total of 305 consecutive patients with angina who underwent diagnostic coronary angiography were enrolled in the study between August 2017 and August 2018. The levels of ANGPTL3 and ANGPTL4 were measured by using competitive ELISA kits. Results According to the degree of coronary artery stenosis, patients were classified into four types: coronary artery stenosis of < 10%, 10-50%, 50-75, and > 75%. The plasma ANGPTL3 level was higher (51.71 ± 52.67 vs. 24.65 ± 10.32 ng/mL, P < 0.001) and that of ANGPTL4 was lower (454.66 ± 269.05 vs. 875.49 ± 961.15 ng/mL, P < 0.001) in the coronary artery stenosis ≥ 10% group than in the < 10% group. ANGPTL3 and ANGPTL4 levels were significantly associated with the severity of coronary vascular stenosis. ROC curve analyses indicated that ANGPTL3 concentrations above 30.5 ng/mL can predict atherosclerosis with a sensitivity of 71.2% and specificity of 75.3%, and that ANGPTL4 levels below 497.5 ng/mL can predict atherosclerosis with a sensitivity of 63.9% and specificity of 74.5%. ANGPTL3 and ANGPTL4 were determined to be independent risk factors for coronary atherosclerosis with odds ratios (ORs) of 0.189 (95% CI 0.097-0.368, P < 0.001) and 3.625 (95% CI 1.873-7.016, P < 0.001), respectively. Conclusions Increased ANGPTL3 or decreased ANGPTL4 shows an association with coronary atherosclerosis and, may become a predictor of coronary atherosclerosis in the future.

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Autoantibodies Against Methylglyoxal-Modified Apolipoprotein B100 and ApoB100 Peptide Are Associated With Less Coronary Artery Atherosclerosis and Retinopathy in Long-Term Type 1 Diabetes

10.2337/figshare.14109509 ◽

2021 ◽

Author(s):

Kari Anne Sveen ◽

Kristine Bech Holte ◽

Mona Svanteson ◽

Kristian F Hanssen ◽

Jan Nilsson ◽

...

Keyword(s):

Type 1 Diabetes ◽

Coronary Artery ◽

Coronary Stenosis ◽

Coronary Atherosclerosis ◽

Microvascular Complications ◽

Proliferative Retinopathy ◽

Inverse Association ◽

Replication Cohort ◽

Coronary Artery Atherosclerosis

Objectives: Methylglyoxal (MGO), a reactive aldehyde forming advanced glycation end products (AGEs), is increased in diabetes and recognized by the immune system, resulting in anti-AGE-specific autoantibodies. The association of these immune responses with macro-and microvascular complications in type 1 diabetes remains unclarified. We investigated associations between MGO-modified ApoB100 and ApoB100 peptide (MGO-p5) autoantibodies and coronary atherosclerosis and retinopathy in type 1 diabetes. Research Design and Methods: IgM and IgG against MGO-apoB100 and MGO-p5 were measured by ELISA in plasma from 103 type 1 diabetessubjects and 63 controls (Dialong study) and in a replication cohort of 27 type 1 diabetes subjects (Oslo study). Coronary atherosclerosis was assessed by computer tomography coronary angiography or intravascular ultrasound. Retinopathy was classified by retinal photos. Results: MGO-ApoB100 IgM and MGO-p5 IgM levels were higher in diabetes subjects with no coronary artery stenosis compared to subjects with significant stenosis (median (IQR): 96.2 AU (71-126.8) vs. 54 AU (36.1-85.4), p=0.003 for MGO-ApoB100, and 77.4 AU (58-106) vs 36.9 AU (28.9-57.4), p=0.005 for MGO-p5). MGO-ApoB100 IgM and MGO-p5 IgM were associated with less severe coronary stenosis after adjusting for confounders (odds ratio (95% CI): <a>0.2 (0.05-0.6), p=0.01 and 0.22 (0.06-0.75), p=0.02</a>. The inverse association of MGO-p5 IgM and coronary stenosis was confirmed in the replication cohort. Subjects with proliferative retinopathy had significantly lower MGO-ApoB100 IgM and MGO-p5 IgM than those with background retinopathy. Conclusions: Autoantibodies against AGE-modified apoB100 are inversely associated with coronary atherosclerosis and proliferative retinopathy, suggesting vascular protective effects of these autoantibodies in type 1 diabetes.

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Autoantibodies Against Methylglyoxal-Modified Apolipoprotein B100 and ApoB100 Peptide Are Associated With Less Coronary Artery Atherosclerosis and Retinopathy in Long-Term Type 1 Diabetes

10.2337/figshare.14109509.v1 ◽

2021 ◽

Author(s):

Kari Anne Sveen ◽

Kristine Bech Holte ◽

Mona Svanteson ◽

Kristian F Hanssen ◽

Jan Nilsson ◽

...

Keyword(s):

Type 1 Diabetes ◽

Coronary Artery ◽

Coronary Stenosis ◽

Coronary Atherosclerosis ◽

Microvascular Complications ◽

Proliferative Retinopathy ◽

Inverse Association ◽

Replication Cohort ◽

Coronary Artery Atherosclerosis

Objectives: Methylglyoxal (MGO), a reactive aldehyde forming advanced glycation end products (AGEs), is increased in diabetes and recognized by the immune system, resulting in anti-AGE-specific autoantibodies. The association of these immune responses with macro-and microvascular complications in type 1 diabetes remains unclarified. We investigated associations between MGO-modified ApoB100 and ApoB100 peptide (MGO-p5) autoantibodies and coronary atherosclerosis and retinopathy in type 1 diabetes. Research Design and Methods: IgM and IgG against MGO-apoB100 and MGO-p5 were measured by ELISA in plasma from 103 type 1 diabetessubjects and 63 controls (Dialong study) and in a replication cohort of 27 type 1 diabetes subjects (Oslo study). Coronary atherosclerosis was assessed by computer tomography coronary angiography or intravascular ultrasound. Retinopathy was classified by retinal photos. Results: MGO-ApoB100 IgM and MGO-p5 IgM levels were higher in diabetes subjects with no coronary artery stenosis compared to subjects with significant stenosis (median (IQR): 96.2 AU (71-126.8) vs. 54 AU (36.1-85.4), p=0.003 for MGO-ApoB100, and 77.4 AU (58-106) vs 36.9 AU (28.9-57.4), p=0.005 for MGO-p5). MGO-ApoB100 IgM and MGO-p5 IgM were associated with less severe coronary stenosis after adjusting for confounders (odds ratio (95% CI): <a>0.2 (0.05-0.6), p=0.01 and 0.22 (0.06-0.75), p=0.02</a>. The inverse association of MGO-p5 IgM and coronary stenosis was confirmed in the replication cohort. Subjects with proliferative retinopathy had significantly lower MGO-ApoB100 IgM and MGO-p5 IgM than those with background retinopathy. Conclusions: Autoantibodies against AGE-modified apoB100 are inversely associated with coronary atherosclerosis and proliferative retinopathy, suggesting vascular protective effects of these autoantibodies in type 1 diabetes.

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High serum copeptin may be a marker of an increased carotid intima-media thickness in asymptomatic patients with type 1 diabetes

Journal of Diabetes and its Complications ◽

10.1016/j.jdiacomp.2021.108085 ◽

2021 ◽

pp. 108085

Author(s):

Lía Nattero-Chávez ◽

María Ángeles Martínez-García ◽

Sandra Redondo López ◽

Elena Fernández-Durán ◽

Beatriz Dorado Avendaño ◽

...

Keyword(s):

Type 1 Diabetes ◽

Intima Media Thickness ◽

Carotid Intima Media Thickness ◽

High Serum ◽

Media Thickness ◽

Asymptomatic Patients

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Hyperhomocysteinemia Measured by Immunoassay: A Valid Measure of Coronary Artery Atherosclerosis

Archives of Pathology & Laboratory Medicine ◽

10.5858/2004-128-1263-hmbiav ◽

2004 ◽

Vol 128 (11) ◽

pp. 1263-1266

Author(s):

Michele T. Stauffenberg ◽

Richard A. Lange ◽

L. David Hillis ◽

Joaquin Cigarroa ◽

Rebecca M. Hsu ◽

...

Keyword(s):

Cardiovascular Disease ◽

Coronary Artery ◽

Coronary Angiography ◽

High Performance ◽

Coronary Artery Stenosis ◽

Artery Stenosis ◽

Valid Measure ◽

Atherosclerotic Burden ◽

Coronary Artery Atherosclerosis ◽

Folate And Vitamin B12

Abstract Context.—Homocysteine is emerging as a novel marker of atherothrombosis. Its role as an independent risk factor for cardiovascular disease is generally accepted. There is scanty data correlating homocysteine levels measured by immunoassay with cardiovascular disease. We previously validated a fluorescence polarization immunoassay for measuring homocysteine, which compared favorably with high performance liquid chromatography. Objective.—To determine if homocysteine levels measured by immunoassay correlate with extent of atherosclerotic burden, as represented by degree of coronary artery stenosis determined by coronary angiography. Design.—Fasting plasma samples were obtained from patients undergoing coronary angiography (N = 165). Homocysteine levels were measured by immunoassay and coronary artery stenosis was determined by coronary angiography. Results.—Median coronary artery stenosis for the 3 homocysteine subgroups, less than 1.35, 1.35 to 6.75, and greater than 6.75 mg/L (<10, 10–15, and >15 μmol/L), was 75%, 90%, and 99%, respectively (P = .01 for trend). Also, folate and vitamin B12 levels decreased with increasing homocysteine levels (P = .01 and .04, respectively, for trend). Spearman's correlation showed a significant association between homocysteine level and coronary artery stenosis (r = 0.20; P = .009). When men and women were examined separately, the correlation was significant only for women (r = 0.30; P = .01). Conclusion.—Homocysteine levels, as measured by immunoassay, show a positive correlation with cardiovascular disease in women. Thus, this is a valid measure of atherosclerotic burden and, therefore, a reliable addition to the established laboratory repertoire for the assessment of cardiovascular disease.

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Asymmetric dimethylarginine levels and diabetes duration: Relationship with measures of subclinical atherosclerosis and cardiac function in children and adolescents with Type 1 diabetes

Diabetes and Vascular Disease Research ◽

10.1177/1479164118757921 ◽

2018 ◽

Vol 15 (3) ◽

pp. 196-203 ◽

Cited By ~ 2

Author(s):

Betül Ersoy ◽

Nilgün Eroğlu ◽

Mecnun Çetin ◽

Ece Onur ◽

Mine Özkol ◽

...

Keyword(s):

Type 1 Diabetes ◽

Asymmetric Dimethylarginine ◽

Intima Media Thickness ◽

Carotid Intima Media Thickness ◽

Tissue Doppler ◽

Diabetes Duration ◽

Media Thickness ◽

Group 3 ◽

Isovolumic Relaxation

Aims: We aimed to evaluate asymmetric dimethylarginine levels in young patients with Type 1 diabetes mellitus according to diabetes duration and to examine the relationship between these levels and measures of atherosclerosis and myocardial function. Materials and methods: In total, 83 patients (8.5–22 years) with Type 1 diabetes mellitus were stratified by diabetes duration: 12–60 months (Group 1, n = 27), >60–120 months (Group 2, n = 29) and >120 months (Group 3, n = 27). Asymmetric dimethylarginine levels were assessed. Carotid intima–media thickness was measured. Myocardial function was assessed by M-mode, conventional Doppler and tissue Doppler echocardiography. Results: Asymmetric dimethylarginine level was significantly higher in Group 1, while carotid intima–media thickness was significantly greater in Group 3 ( p < 0.05). Tissue Doppler echocardiography showed the ratio of peak early to peak late diastolic myocardial annular velocity decreased significantly in Groups 2 and 3 with a negative correlation with duration (r: −0.310, p = 0.004) and HBA1c levels (r = −0.391, p < 0.001). Myocardial performance index in all groups and isovolumic relaxation time in Group 3 increased significantly. Asymmetric dimethylarginine levels were negatively correlated with carotid intima–media thickness and isovolumic relaxation time ( p < 0.05). Conclusion: In contrast to adult diabetics, asymmetric dimethylarginine concentration decreases as diabetes duration increases in young Type 1 diabetic patients and is associated with worsening measures of cardiovascular risk and poorer diastolic function.

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