Molecular sex determination of 20 bird species protected in the Republic of Serbia

Molecular sex determination of five varieties of Indonesian papaya were investigated using RAPD (Random Amplified Polymorphism DNA) markers. Overall, 12 of 14 primers produced polymorphic bands on either several or all tested varieties. The OPC04 and RAPD2 markers could be used determined sex types on all varieties, whereas others primers are only on certain varieties. The Tangkai Ungu variety can be differentiate by markers: OPA11, OPA14, OPC14, RAPD2, RAPD3, and RAPD5; the Lokal Sumani can be determine using markers: OPA01, OPA11, OPA14, OPC01, OPC04, RAPD2, RAPD3, RAPD5, and RAPD6; the Merah Delima could be determined using OPC04, OPN09, RAPD2, and RAPD5; the Dampit could be determined using OPC01, OPC04, RAPD2, and RAPD6; whereas the Sicincing Panjang could be determined using OPA04, OPA11, OPA14, OPC04, RAPD2, and RAPD3.

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Hormone Mass Fingerprinting: Novel Molecular Sex Determination of Ancient Human Skeletal Remains

Proceedings of the 37th International Symposium on Archaeometry, 13th - 16th May 2008, Siena, Italy ◽

10.1007/978-3-642-14678-7_59 ◽

2010 ◽

pp. 411-415 ◽

Cited By ~ 1

Author(s):

L. Mark ◽

Z. Patonai ◽

A. Vaczy ◽

N. Kajsza ◽

A. Marcsik

Keyword(s):

Sex Determination ◽

Skeletal Remains ◽

Human Skeletal Remains ◽

Molecular Sex Determination

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Morphological Characterisation And Molecular Sex Determination Of Human Remains From The 15th–17th Centuries In Latvia

Proceedings of the Latvian Academy of Sciences Section B Natural Exact and Applied Sciences ◽

10.2478/prolas-2014-0013 ◽

2015 ◽

Vol 69 (1-2) ◽

pp. 8-13 ◽

Cited By ~ 1

Author(s):

Asta Ščėsnaitė-Jerdiakova ◽

Liāna Pliss ◽

Guntis Gerhards ◽

Elīna Pētersone Gordina ◽

Agnija Gustiņa ◽

...

Keyword(s):

Sex Determination ◽

Dna Analysis ◽

Molecular Genetic ◽

Skeletal Remains ◽

Human Skeletal Remains ◽

Full Agreement ◽

Archaeological Material ◽

Molecular Approaches ◽

Molecular Sex Determination

Abstract Sex determination is one of the most important and initial steps in human profile identification from archaeological material. The aim of the current study was to evaluate the application of molecular approaches alongside morphological methods for sex determination in archaeological human skeletal remains. Human skeletal remains were excavated from three cemeteries: St Gertrude Old Church, Dom Square and St Peter’s Church, of 15th–17th century burials in Rīga, Latvia. Morphological and molecular genetic methods, including amplification of genes AMELX/Y and SRY were used to analyse seven skeletal remains. The conducted analyses of morphological features identified sex in all seven cases (two females and five males). By molecular analyses of mediaeval DNA it was possible to determine sex in five of seven (71%) samples. In all positive cases full agreement between morphological estimation and molecular genetic methods was observed. To conclude, DNA analysis can be considered for sex identification in cases with no signs of sexual dimorphism (juvenile skeletons) or partially preserved skeletons.

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DNA sexing for gender determination of Changeable Hawk-Eagle (Nisaetus cirrhatus, Gmelin, 1788)

E3S Web of Conferences ◽

10.1051/e3sconf/202124903012 ◽

2021 ◽

Vol 249 ◽

pp. 03012

Author(s):

Annisa ◽

Mariana Fikriyanti ◽

Susanti Withaningsih

Keyword(s):

Sex Ratio ◽

Sex Determination ◽

Natural Habitat ◽

Bird Species ◽

Morphometric Data ◽

Breeding Programs ◽

Blood Samples ◽

Gender Determination ◽

Natural Behaviour

The Changeable hawk-eagle (Brontok eagle) is a protected bird species. It is one of the most frequently hunted and traded birds in Indonesia. The processes of being traded changes this bird natural behaviour. Therefore, a rehabilitation effort to return the eagle’s behaviour to conform to its natural habits is needed. The ultimate goal of rehabilitation is to release the changeable hawk-eagle back into its natural habitat. In conservation and breeding programs, efforts to determine the sex of eagles to be released are very important to help increase the population of changeable hawk-eagles in their habitat by looking at the sex ratio. At the present, sex determination at the Kamojang Conservation Eagle Center (Pusat Konservasi Elang Kamojang or PKEK) uses the morphometric method. This research used the DNA sexing method with primers 2550F and 2718R to determine the sex of Changeable hawk-eagles in PKEK by extracting DNA from blood samples of 30 eagles. Comparison of DNA sexing results and morphometric data showed differences. This proves that DNA sexing, is suitable in determining changeable hawk-eagles’ sex.

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Time and Latitude in South Africa

International Astronomical Union Colloquium ◽

10.1017/s0252921100026816 ◽

1972 ◽

Vol 1 ◽

pp. 27-38

Author(s):

J. Hers

Keyword(s):

South Africa ◽

Cape Town ◽

Astronomical Observations ◽

Royal Observatory ◽

The Republic ◽

Astronomical Determination ◽

Limited Accuracy ◽

Better Than

In South Africa the modern outlook towards time may be said to have started in 1948. Both the two major observatories, The Royal Observatory in Cape Town and the Union Observatory (now known as the Republic Observatory) in Johannesburg had, of course, been involved in the astronomical determination of time almost from their inception, and the Johannesburg Observatory has been responsible for the official time of South Africa since 1908. However the pendulum clocks then in use could not be relied on to provide an accuracy better than about 1/10 second, which was of the same order as that of the astronomical observations. It is doubtful if much use was made of even this limited accuracy outside the two observatories, and although there may – occasionally have been a demand for more accurate time, it was certainly not voiced.

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