scholarly journals DNA sexing for gender determination of Changeable Hawk-Eagle (Nisaetus cirrhatus, Gmelin, 1788)

2021 ◽  
Vol 249 ◽  
pp. 03012
Author(s):  
Annisa ◽  
Mariana Fikriyanti ◽  
Susanti Withaningsih
Keyword(s):  
Sex Ratio ◽  
Sex Determination ◽  
Natural Habitat ◽  
Bird Species ◽  
Morphometric Data ◽  
Breeding Programs ◽  
Blood Samples ◽  
Gender Determination ◽  
Natural Behaviour

The Changeable hawk-eagle (Brontok eagle) is a protected bird species. It is one of the most frequently hunted and traded birds in Indonesia. The processes of being traded changes this bird natural behaviour. Therefore, a rehabilitation effort to return the eagle’s behaviour to conform to its natural habits is needed. The ultimate goal of rehabilitation is to release the changeable hawk-eagle back into its natural habitat. In conservation and breeding programs, efforts to determine the sex of eagles to be released are very important to help increase the population of changeable hawk-eagles in their habitat by looking at the sex ratio. At the present, sex determination at the Kamojang Conservation Eagle Center (Pusat Konservasi Elang Kamojang or PKEK) uses the morphometric method. This research used the DNA sexing method with primers 2550F and 2718R to determine the sex of Changeable hawk-eagles in PKEK by extracting DNA from blood samples of 30 eagles. Comparison of DNA sexing results and morphometric data showed differences. This proves that DNA sexing, is suitable in determining changeable hawk-eagles’ sex.

The reproductive habits and sex-determination of the nematode Rhabditis pellio Bütschli, with a note on its taxonomy and nomenclature

Parasitology ◽  
1953 ◽  
Vol 43 (1-2) ◽  
pp. 94-101 ◽  
Author(s):  
D. A. T. New
Keyword(s):  
Sex Ratio ◽  
Sex Determination ◽  
Natural Conditions ◽  
Separate Species ◽  
Chromosomal Arrangement ◽  
Parthenogenetic Reproduction ◽  
Rhabditis Pellio

1. The nematode Rhabditis pellio Bütschli may be cultured indefinitely on earthworm extract.2. Although the females of this nematode are usually capable of parthenogenetic reproduction, males are produced in the offspring only if copulation has taken place.3. Occasionally non-parthenogenetic generations appear but these do not represent a separate species.4. An explanation is given for the sex ratio found under natural conditions and it is suggested that sex-determination works on a chromosomal arrangement of XO = male and XX = female.5. The nematode named R. terrestris Stephenson 1942 is probably the same as that named R. pellio Bütschli by Johnson 1913.6. The nomenclature of R. pellio Bütschli is discussed.

scholarly journals PENENTUAN JENIS KELAMIN BURUNG KENARI (Serinus canaria) BERDASARKAN GEN Chromodomain Helicase DNA-Binding 1 (CHD1)

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Afif Muhammad Akhrom ◽  
Indarjulianto Soedarmanto ◽  
Yanuartono Yanuartono ◽  
Trini Susmiati ◽  
Alfarisa Nururrozi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Sex Determination ◽  
Mature Female ◽  
Mature Male ◽  
Chain Reaction ◽  
Blood Samples ◽  
Pcr Method ◽  
Polymerase Chain ◽  
Female Canaries

Phenotype determination of sex in young canaries is very low in accuracy. This study aimed to develop a genotypic sexing method in canaries. This study used 12 canaries consisting of 3 mature males, 3 mature females and 6 one-month-old canaries. Phenotypic sexing by cloacal observation was done on all birds, continued by genotypic sexing to identification CHD1 gene using polymerase chain reaction (PCR). The PCR used blood samples for mature canaries, and feather for mature and one-month-old canaries. The results of phenotypic observations showed that all mature male canaries had prominent and pointed cloaca forms, all mature females had flat and wide, whereas all one-month-old birds had a flat cloaca. The result of PCR showed a single band (500 bp) for mature male and double bands (500 bp and 300 bp) for mature female canaries. The PCR results of one-month-old canaries showed that there were one male and five females. Based on this study, it was concluded that genotypic sexing using the PCR method is effective in the sex determination of canaries.Keywords: canary, CHD1, genotype, PCR, sexing ABSTRAKPenentuan jenis kelamin burung kenari muda secara fenotip akurasinya sangat rendah. Penelitian ini bertujuan untuk menentukan jenis kelamin burung kenari secara genotip. Penelitian ini menggunakan 12 ekor burung kenari, terdiri dari 6 ekor dewasa (3 jantan, 3 betina) serta 6 ekor umur 1 bulan. Semua burung ditentukan jenis kelaminnya dengan mengamati kloaka dan identifikasi gen CHD1 menggunakan teknik polymerase chain reaction (PCR). Sampel DNA berasal dari darah dan bulu untuk burung dewasa serta bulu untuk burung umur 1 bulan. Pengamatan fenotip menunjukkan bahwa burung kenari dewasa jantan mempunyai bentuk kloaka menonjol dan runcing, dewasa betina berbentuk datar dan lebar, sedangkan semua burung umur 1 bulan mempunya bentuk kloaka datar. Hasil identifikasi gen CHD1 diperoleh adanya 1 pita gen sekitar 500 bp dari sampel darah dan bulu semua burung kenari dewasa jantan, dan 2 pita gen sekitar 500 bp dan 300 bp dari sampel semua burung kenari betina dewasa. Hasil PCR pada sampel burung umur 1 bulan menunjukkan bahwa 1 ekor jantan dan 5 ekor betina. Berdasarkan penelitian ini dapat disimpulkan bahwa penentuan jenis kelamin secara genotip menggunakan gen CHD1 dapat dilakukan pada burung kenari.

scholarly journals Assessment of seed quality and sex determination in hop seedlings using molecular markers

2020 ◽  
Vol 42 ◽  
pp. e45
Author(s):  
Marília Pereira Machado ◽  
Andreza Cerioni Belniaki ◽  
André Felipe Bernert ◽  
Erik Nunes Gomes ◽  
João Carlos Bespalhok Filho ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Seed Quality ◽  
Germination Percentage ◽  
Brewing Industry ◽  
Breeding Programs ◽  
Normal Seedling ◽  
Physiological Quality ◽  
Male Specific

Brazil is the world's third largest beer consumer and currently imports all of its hops for the brewing industry. Such a fact justifies the selection of hop genotypes adapted for cultivation locally, which requires high quality seeds and efficient sex determination of the seedlings. The objectives of this study were to develop a methodology to assess hop seed quality and to efficiently determine hop seedling sex through the use of male-specific molecular markers. Freshly harvested hop seeds were germinated with and without pre-chilling (3-5 ° C) for 3, 6 and 12 weeks and then germinated at 20 or 25 ° C in the presence or absence of light, evaluating germination percentage and germination speed index. F1 progenies were obtained from after seed germination in a greenhouse and seedlings sex was determined using male-specific molecular markers. The best conditions for physiological quality assessment of hop seeds used in the present study were pre-chilling for 12 weeks, followed by germination at 25 ° C, and normal seedling counts at 7 and 15 days. The progeny submitted to molecular marker sexing was composed of 61.3% female plants. The established methodologies presented here can be considered efficient and may contribute to expedite hops breeding programs.

Identification and sex determination of Bicknell's Thrushes using morphometric data

2008 ◽  
Vol 79 (4) ◽  
pp. 408-420 ◽  
Author(s):  
Sarah J. K. Frey ◽  
Christopher C. Rimmer ◽  
Kent P. McFarland ◽  
Stéphane Menu
Keyword(s):  
Sex Determination ◽  
Morphometric Data

Sex Ratio and Sex Determination of Immature Brown-Headed Cowbirds

Bird-Banding ◽  
1976 ◽  
Vol 47 (2) ◽  
pp. 112 ◽  
Author(s):  
Richard A. Hill
Keyword(s):  
Sex Ratio ◽  
Sex Determination

scholarly journals Molecular sex determination of 20 bird species protected in the Republic of Serbia

2013 ◽  
Vol 63 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Marija Stevanov-Pavlovic ◽  
M. Vucicevic ◽  
Jasna Bosnjak ◽  
Jevrosima Stevanovic ◽  
V. Dimitrijevic ◽  
...  
Keyword(s):  
Sex Determination ◽  
Bird Species ◽  
The Republic ◽  
Molecular Sex Determination

scholarly journals Response of Superovulation by Using FSH (Follicle Stimulating Hormone) and Sex Determination of Embryos Using PCR in Pesisir Cows of West Sumatra

2019 ◽  
Vol 20 (1) ◽  
pp. 7
Author(s):  
Tinda Afriani ◽  
Fery Lismanto Sayful ◽  
Sumedi Sumedi ◽  
Dino Eka Putra ◽  
Endang Purwati
Keyword(s):  
Drug Release ◽  
Sex Ratio ◽  
Sex Determination ◽  
Corpus Luteum ◽  
Follicle Stimulating Hormone ◽  
West Sumatra ◽  
Natural Mating ◽  
Stimulating Hormone

This study  was conducted to determine the response of superovulation by giving 16 ml dosage  of FSH hormone to female Pesisir cattle.  The estrus schedule of 15 Pesisir cows was set by inserting CIDR (Controlled Internal Drug Release) into the vagina for 12 days. At day 10, all cattles were injected with FSH for three consequent days but with decreasing dosage. On the 3rd day, FSH injection was accompanied by PGF2α injection and CIDR was removed. The detection of estrus was performed at  day 13. Natural mating was proceeded after the estrus signs visible. Collection of donor embryos was done on the 6th and 8th day after mating. The variables  measured were the response of superovulation, total number of corpus luteum, number of embryos and sex ratio. The results obtained were all  Pesisir cows responded to superovulation. The  average number of of corpus luteum and embryoes per cow were  5.93±3.17 and -----, respectively,  while the total of transferable embryoes were 90, with an average of 6.00 or 61.64%. The sexing of embryoes obtained in this study from 146 embryoes awere 76.03% males (111 embryoes) and 23.97% females (35 embryoes). Based on total of transferable embryoes, there were 51.37% male embryos and 11.28% of females embryos. The result of this study showed that the sex ratio of male embryos was higher than female embryos.

scholarly journals The Application of UTY and SRY Molecular Markers for Determination of Unknown Sex Samples in Bali Cattle

2020 ◽  
Vol 21 (1) ◽  
pp. 55
Author(s):  
Indriawati Indriawati ◽  
Slamet Diah Volkandari ◽  
Endang Tri Margawati
Keyword(s):  
Molecular Markers ◽  
Sex Determination ◽  
Accurate Information ◽  
Blood Samples ◽  
Unknown Sample ◽  
Pooled Dna ◽  
Pcr Method ◽  
Bali Cattle ◽  
Dna Template

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.

SEX DETERMINATION OF FETUS PRENATAL FROM MATERNAL PLASMA BY USING DUPLEX PCR TECHNIQUE IN GOAT

2014 ◽  
Vol 13 (1) ◽  
pp. 50-59
Author(s):  
A NisreenYasirJasim ◽  
Tahir A. Fahid ◽  
Talib Ahmed Jaayid
Keyword(s):  
Sex Determination ◽  
Maternal Plasma ◽  
Duplex Pcr
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