scholarly journals Confined placental mosaicism in short term culture

Genetika ◽  
2016 ◽  
Vol 48 (3) ◽  
pp. 955-961
Author(s):  
Bojana Petrovic ◽  
Jelena Dukanac-Stamenkovic
Keyword(s):  
Genetic Counseling ◽  
Perinatal Care ◽  
Chromosome Analysis ◽  
Chromosomal Mosaicism ◽  
Special Importance ◽  
Short Term ◽  
Chorionic Villi ◽  
Additional Chromosome ◽  
Placental Mosaicism ◽  
Short Term Culture

Finding of fetal chromosomal mosaicism complicates genetic counseling, as well as pregnancy management. The aim of this study was to determine the risk of confined placental mosaicism in short term culture of chorionic villous samples. We conducted a retrospective review of karyotype analysis results obtained after chorionic villous sampling (CVS) in two years period. A 420 samples of chorionic villi were taken transabdominally and obtained by a semidirect method (overnight incubating culture). All fetuses with CVS mosaicism were under the intensive perinatal care. In all cases of chromosome mosaicism the additional karyotyping was performed from fetal blood samples after 22nd gestational week in order to exclude true fetal mosaicism. After delivery newborns were examined by experienced pediatrician. From 420 analyzed samples in 11 (2,6%) cases we found placental mosaicism. No anomalies were seen in genetic sonogram of this fetuses and mosaicism was confirmed only in one case. Confined placental mosaicism (CPM) was found in 2,1% (9/420) of all analyzed cases, and it made 90% of all placental mosaicism. In 60% (6/10) of placental mosaicism cases we found mosaicism with single aberrant cell. Trisomy 21 mosaicism was the most frequent aberration found in 30% of cases. Finding of mosaicism in chorionic villi sample is at special importance for genetic counseling, because every case has to be reveled individually regarding the type and level of mosaicism. Anyway, in every case of placental mosaicism intensive antenatal monitoring is necessary, with additional chromosome analysis from different tissue in consideration of previous findings.

scholarly journals Overview of Chromosome Abnormalities in First Trimester Miscarriages: A Series of 1,011 Consecutive Chorionic Villi Sample Karyotypes

2017 ◽  
Vol 152 (2) ◽  
pp. 81-89 ◽  
Author(s):  
Anna Soler ◽  
Carme Morales ◽  
Irene Mademont-Soler ◽  
Ester Margarit ◽  
Antoni Borrell ◽  
...  
Keyword(s):  
Maternal Age ◽  
First Trimester ◽  
Chromosome Abnormalities ◽  
Chorionic Villi ◽  
Autosomal Trisomy ◽  
Different Types ◽  
Placental Mosaicism ◽  
Short Term Culture ◽  
Maternal Contamination

In order to contribute to the knowledge of type and frequency of chromosome abnormalities in early pregnancy losses, we analyzed the cytogenetic results from a large series of first trimester miscarriages, using a diagnostic approach with a high success rate and no maternal contamination. A total of 1,119 consecutive chorionic villi samples were obtained before evacuation, and karyotypes were prepared after short-term culture (STC). In 603 samples, a long-term culture (LTC) was also performed. The overall and individual frequencies of the different types of chromosome abnormalities were established, including placental mosaicisms, and their relationship with maternal age and gestational weeks was assessed. An abnormal karyotype was detected in 70.3% of the samples. Single autosomal trisomy was the most frequent abnormality (64.6% of the abnormal cases), followed by triploidy (13.1%) and monosomy X (10.4%). Chromosome rearrangements were found in 5.2%, combined abnormalities in 8.9%, and placental mosaicism in 3.5% of the cases with STC and LTC performed. Individual trisomies behaved differently with respect to maternal age and intrauterine survival. Due to the combination of STC and LTC, our study offers reliable information on the incidence and type of chromosome abnormalities and placental mosaicism in miscarriages and contributes to define the cytogenetic implication in their etiology.

scholarly journals P243: False negative result in chorionic villi short term culture missing a translocation trisomy 21

2003 ◽  
Vol 22 (S1) ◽  
pp. 135-135
Author(s):  
M. Entezami ◽  
S. Yildirim ◽  
S. Hese ◽  
M. Stumm ◽  
R. D. Wegner
Keyword(s):  
Trisomy 21 ◽  
False Negative ◽  
False Negative Result ◽  
Short Term ◽  
Chorionic Villi ◽  
Short Term Culture

Direct and short-term culture preparation of chorionic villi. Is any one method best?

1988 ◽  
Vol 8 (7) ◽  
pp. 501-509 ◽  
Author(s):  
D. S. Holmes ◽  
A. M. Fifer ◽  
W. E. Mackenzie ◽  
M. J. Griffiths ◽  
J. R. Newton
Keyword(s):  
Short Term ◽  
Chorionic Villi ◽  
Short Term Culture

The proportion of erythroid mitoses in normal human bone marrow in short-term culture systems

Pathology ◽  
1989 ◽  
Vol 21 (3) ◽  
pp. 185-188 ◽  
Author(s):  
Christina Rudduck ◽  
O. Margaret Garson
Keyword(s):  
Bone Marrow ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Short Term ◽  
Culture Systems ◽  
Normal Human ◽  
Normal Human Bone Marrow ◽  
Short Term Culture

Evaluation of individual sensitivity of head and neck squamous cell carcinoma to cetuximab by short-term culture of tumor slices

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E911-E915 ◽  
Author(s):  
Marine Peria ◽  
Jérôme Donnadieu ◽  
Caroline Racz ◽  
Jean-Fortuné Ikoli ◽  
Antoine Galmiche ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Neck Squamous Cell Carcinoma ◽  
Individual Sensitivity ◽  
Short Term ◽  
Short Term Culture

scholarly journals Growth inhibition of follicular small-cleaved-cell lymphoma cells in short-term culture by interleukin-3

1994 ◽  
Vol 5 (3) ◽  
pp. 265-268 ◽  
Author(s):  
A. Younes ◽  
J. Drach ◽  
R. Katz ◽  
D. Jendiroba ◽  
M.H. Sabourian ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cell Lymphoma ◽  
Short Term ◽  
Lymphoma Cells ◽  
Interleukin 3 ◽  
Short Term Culture

Morphologic and physiologic studies of canine ileal enteroglucagon-containing cells in short-term culture

1987 ◽  
Vol 93 (4) ◽  
pp. 791-800 ◽  
Author(s):  
A.M.J. Buchan ◽  
D.L. Barber ◽  
M. Gregor ◽  
A.H. Soll
Keyword(s):  
Short Term ◽  
Short Term Culture

Short-term Culture of Epithelial Cells from Urine of Adults

1979 ◽  
Vol 161 (2) ◽  
pp. 153-157 ◽  
Author(s):  
F. Herz ◽  
A. Schermer ◽  
L. G. Koss
Keyword(s):  
Epithelial Cells ◽  
Short Term ◽  
Short Term Culture

scholarly journals PECULIARITIES OF ALLOGENIC INTERACTIONS IN THE SHORT-TERM CULTURE OF LYMPHOCYTES OF SPOUSES WHO HAVE CHILDREN WITH CONGENITAL HEART DISEASES OR EARLY REPRODUCTIVE LOSSES

2019 ◽  
Vol 21 (2) ◽  
pp. 279-292 ◽  
Author(s):  
A. V. Shabaldin ◽  
S. A. Shmulevich ◽  
G. N. Chistyakova ◽  
I. I. Remizova ◽  
E. B. Lukoyanycheva ◽  
...  
Keyword(s):  
Immune Response ◽  
Mixed Culture ◽  
Congenital Heart Defects ◽  
Congenital Heart ◽  
Heart Defects ◽  
Married Couples ◽  
Healthy Children ◽  
Short Term ◽  
Reproductive Losses ◽  
Short Term Culture

We have studied HLA allogeneic interactions in short-term cultures of lymphocytes from the parents having children with congenital heart defects (CHD), or subject to early reproductive losses. Twentyone married couples (CHD as the main group) who had children with sporadic CHD (interventricular septal defect) without chromosomal diseases were observed. Fifty married couples (a comparison group) had two or more reproductive losses in early gestation (up to 9 weeks), denoted as PNPs (miscarriages, missed abortions, habitual miscarriages. Forty-one families with three or more healthy children represented a control group. Immune response in cell cultures was evaluated by increasing expression of HLA-DR in a mixed culture, as compared to spontaneous lymphocyte cultures. Initial labeling of female and male lymphocytes with monoclonal antibodies to CD45 conjugated to different fluorescent dyes (PC-5 and PC-7) allowed us to evaluate the immune response of female lymphocytes to males and vice versa. The suppressor effect of autologous female serum upon the mixed culture of the lymphocytes of the spouses was also evaluated. Results of the present study showed a difference in HLA allogeneic interactions in the short-term culture of lymphocytes registered for spouses with reproductive losses and children with congenital heart defects. Reproductive losses were associated with a low blocking effect of female auto-serum upon allogeneic HLA interactions in the short-term culture of the lymphocytes of the spouses. Congenital heart defects were associated with high activity of female B-lymphocytes (CD3-/HL-DR+) in short-term mixed culture of lymphocytes from the spouses.

scholarly journals A unique pattern of central nervous system leukemia in acute myelomonocytic leukemia associated with inv(16)(p13q22)

Blood ◽  
1985 ◽  
Vol 65 (5) ◽  
pp. 1071-1078
Author(s):  
R Holmes ◽  
MJ Keating ◽  
A Cork ◽  
Y Broach ◽  
J Trujillo ◽  
...  
Keyword(s):  
Median Duration ◽  
Myeloid Leukemia ◽  
Chromosome Analysis ◽  
Leptomeningeal Disease ◽  
Brain Scan ◽  
Fab Classification ◽  
Additional Chromosome ◽  
Acute Myelomonocytic Leukemia ◽  
Immature Cells ◽  
Myelomonocytic Leukemia

Twenty-six patients with inv(16)(p13q22) or del(16)(q22) in association with acute myelomonocytic leukemia (AMML-M4, FAB classification), and abnormal marrow eosinophils have been treated at this institute. Initial bone marrow eosinophilia (greater than or equal to 4%) was observed in 22 of 26 patients (85%), and abnormal eosinophil morphology, characterized by immature cells with some interspersed basophilic granules, was evident in 26 of 26 (100%). Giemsa-banded chromosome analysis performed in all patients revealed 16 cases with inv(16)(p13q22) alone, and ten cases with additional chromosome changes. Twenty-five patients received combination induction chemotherapy, and 23 (92%) achieved complete remission (CR). The median duration of remission was 18 months (range, six to 72 + months), and the median duration of survival was 34 months (range, 0.5 to 133 months). Nine patients (35%) relapsed in the CNS at a median time of 19 months (range, six to 133 months) from first marrow CR. All patients had leptomeningeal disease, and in addition, six of nine (66%) demonstrated two or more enhancing lesions on computed tomography brain scan, consistent with intracerebral myeloblastomas. Review of 384 Giemsa-banded patients with acute myeloid leukemia revealed no other morphologic or cytogenetic subgroup with either an equivalent incidence of CNS leukemia or documented intracerebral myeloblastomas. This series of inv(16)(p13q22)/del(16)(q22) AMML reports a favorable prognosis for such patients and associates a specific clonal cytogenetic subgroup of acute leukemia with a distinct propensity for CNS relapse, manifesting as leptomeningeal disease and intracerebral myeloblastomas.

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