scholarly journals Genetic diversity of Turkish lathyrus L. landraces using ISSR markers

Genetika ◽  
2018 ◽  
Vol 50 (2) ◽  
pp. 395-402
Author(s):  
Hüseyin Uysal ◽  
Zeki Acar ◽  
İlknur Ayan ◽  
Orhan Kurt
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Genetic Relationships ◽  
Issr Markers ◽  
The Other ◽  
High Similarity ◽  
Pairwise Genetic Distance ◽  
Dna Fragment ◽  
Lathyrus Sativus L ◽  
Issr Primers

Fifty-one Lathyrus sativus L. landraces and one L. clymenum L. landrace collected from Turkey and one L. sativus cultivar, G?rb?z, were evaluated with ISSR markers in this study to molecular characterization. Three ISSR primers were used and 45 DNA fragment were evaluated, of which 44 bands were polymorphic. The frequencies of scored bands ranged from 0.009 to 0.888 and averaged 0.363. The genotypes MLT04 and NEV02 had high similarity with 0.825 according to pairwise grouping. The furthest pairwise group was G?rb?z and MLT02 with 0.244. The nearest genotype to G?rb?z was CNR03 with 0.577. The pairwise genetic distance between L. clymenum and L. sativus ranged from 0.353 (accession NEV01) to 0.637 (accession DEN04) and pairwise genetic distance to the cultivar G?rb?z was 0.375. Assessment of genetic relationships among Lathyrus genotypes made two main groups. One of them covered only G?rb?z variety and the other covered 52 Lathyrus landrace. L. sativus and L. clymenum separated prominently in the second group.

scholarly journals Genetic Diversity Based on ISSR Markers of Apple Genotypes in Ardahan/Turkey

2018 ◽  
Vol 10 (4) ◽  
pp. 554-558
Author(s):  
Emre SEVİNDİK ◽  
Hüseyin UYSAL ◽  
Zehra Tuğba MURATHAN
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Distance ◽  
Uv Light ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Genetic Distances ◽  
The Matrix ◽  
Commercial Kits ◽  
Issr Primers

Within the present study, it was conducted a genetic diversity analysis using ISSR markers for some apple genotypes grown in Ardahan region, Turkey. Total genomic DNA (gDNA) isolation from apple leaves was performed using commercial kits. Five ISSR primers were used to determine the genetic diversity among the genotypes studied. Polymerase Chain Reaction (PCR) was performed with all gDNA samples to produce bands to score. PCR products were run in agarose gel and visualized under UV light. Bands on the gels were scored as “1”, while no bands at the corresponding positions were scored as “0”, to generate the matrix file. Five ISSR primers produced a total of 35 bands, and 20 of them were polymorphic. The polymorphic bands rated approximately 57%. Phylogenetic relationships and genetic distances between the genotypes were calculated by using the PAUP [Phylogenetic Analysis Using Parsimony (and Other Methods)] program.  According to the PAUP data, the closest genetic distance was 0.03704 between ‘Kaburga’ and ‘Japon Apple’ genotypes, while the furthest genetic distance was 0.48148 between ‘Karanfil Apple’ and ‘Sisli Uruset’. The phylogenetic analysis obtained using UPGMA algorithm produced a phylogenetic tree with two clades. The results suggest that ISSR markers are useful tools for determining genetic relationships among apple genotypes.

scholarly journals Analysis of genetic relationships of genotypes of the genus Rosa L. from the collection of Nikita Botanical Gardens using ISSR and IRAP DNA markers

2020 ◽  
Vol 24 (5) ◽  
pp. 474-480
Author(s):  
I. I. Suprun ◽  
S. A. Plugatar ◽  
I. V. Stepanov ◽  
T. S. Naumenko
Keyword(s):  
Genetic Diversity ◽  
Bayesian Methods ◽  
Dna Markers ◽  
Wild Species ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
The Other ◽  
Clustering Methods ◽  
Botanical Gardens

In connection with the development of breeding and the creation of new plant varieties, the problem of their genotyping and identification is becoming increasingly important, therefore the use of molecular methods to identify genetic originality and assess plant genetic diversity appears to be relevant. As part of the work performed, informative ISSR and IRAP DNA markers promising for the study of genetic diversity of the Rosa L. genus were sought and applied to analysis of genetic relationships among 26 accessions of the genus Rosa L. from the gene pool collection of Nikita Botanical Gardens. They included 18 cultivated varieties and 8 accessions of wild species. The species sample included representatives of two subgenera, Rosa and Platyrhodon. The subgenus Platyrhodon was represented by one accession of the species R. roxburghii Tratt. Cultivated roses were represented by varieties of garden groups hybrid tea, floribunda, and grandiflora. The tested markers included 32 ISSRs and 13 IRAPs. Five ISSR markers (UBC 824, ASSR29, 3A21, UBC 864, and UBC 843) and three IRAPs (TDK 2R, Сass1, and Сass2) were chosen as the most promising. They were used for genotyping the studied sample of genotypes. In general, they appeared to be suitable for further use in studying the genetic diversity of the genus Rosa L. The numbers of polymorphic fragments ranged from 12 to 31, averaging 19.25 fragments per marker. For markers UBC 864 and UBC 843, unique fingerprints were identified in each accession studied. The genetic relationships of the studied species and varieties of roses analyzed by the UPGMA, PCoA, and Bayesian methods performed on the basis of IRAP and ISSR genotyping are consistent with their taxonomic positions. The genotype of the species R. roxburghii of the subgenus Platyrhodon was determined genetically as the most distant. According to clustering methods, the representative of the species R. bengalensis did not stand out from the group of cultivated varieties. When assessing the level of genetic similarity among the cultivated varieties of garden roses, the most genetically isolated varieties were ‘Flamingo’, ‘Queen Elizabeth’, and ‘Kordes Sondermeldung’; for most of the other varieties, groups of the greatest genetic similarity were identified. This assessment reflects general trends in phylogenetic relationships, both among the studied species of the genus and among cultivated varieties.

scholarly journals Study of genetic differences among Slovak Tsigai populations using microsatellite markers

2009 ◽  
Vol 54 (No. 10) ◽  
pp. 468-474 ◽  
Author(s):  
S. Kusza ◽  
E. Gyarmathy ◽  
J. Dubravska ◽  
I. Nagy ◽  
A. Jávor ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Mating System ◽  
Population Differentiation ◽  
Genetic Relationships ◽  
The Other ◽  
Potential Risks ◽  
Substantial Degree

In this study genetic diversity, population structure and genetic relationships of Tsigai populations in Slovakia were investigated using microsatellite markers. Altogether 195 animals from 12 populations were genotyped for 16 microsatellites. 212 alleles were detected on the loci. The number of identified alleles per locus ranged from 11 to 35. In the majority of the populations heterozygosity deficiency and potential risks of inbreeding could be determined. High values of <I>F</I><sub>ST</sub> (0.133) across all the loci revealed a substantial degree of population differentiation. The estimation of genetic distance value showed that the Slovak Vojin population was the most different from the other populations. The 12 examined populations were able to group into 4 clusters. With this result our aim is to help the Slovak sheep breeders to establish their own mating system, to avoid genetic loss and to prevent diversity of Tsigai breed in Slovakia.

scholarly journals Genetic Diversity of Elaeagnus angustifolia L. (Elaeagnaceae) Populations in İzmir (Turkey)

2021 ◽  
Vol 78 (1) ◽  
pp. 35
Author(s):  
Erengül SOFYALIOĞLU ◽  
Emre SEVİNDİK ◽  
Hüseyin UYSAL
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Trees ◽  
Uv Light ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Genetic Distances ◽  
Elaeagnus Angustifolia ◽  
A Value ◽  
Pcr Products ◽  
Issr Primers

This study was performed out genetic diversity of some Elaeagnus angustifolia L. populations growing in İzmir province by using ISSR markers. In the study, PCR was performed using 15 ISSR primers. PCR products were run in agarose gel and visualized under UV light. Amplified products were scored as follows. A total of 46 bands were produced from 15 ISSR primers, of which 27 were polymorphic. The proportion of polymorphic bands was evaluated as approximately 58.7%. Genetic distances between phylogenetic trees and genotypes were calculated using the PAUP program. The phylogenetic tree consists of two large clades. The longest distance between populations was between Gümüldür-Özdere and Çeşme-Alaçatı population with a value of 0.50, while the closest distance was between Çeşme-Ayayorgi and Konak-Hatay populations with a value of 0.06. The results show that ISSR markers are useful tools for determining genetic relationships between E. angustifolia populations

Genetic relationships of Chinese prickly ash as revealed by ISSR markers

Biologia ◽  
2015 ◽  
Vol 70 (1) ◽  
Author(s):  
Shijing Feng ◽  
Tuxi Yang ◽  
Xiao Li ◽  
Lv Chen ◽  
Zhenshan Liu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationship ◽  
Genetic Divergence ◽  
Genetic Similarity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Chinese Prickly Ash ◽  
New Cultivars ◽  
Issr Primers

AbstractChinese prickly ash, belonging to the genus Zanthoxylum L., has been one of the most important commerciallyexploited plants for its alimentary, industrial and medicinal applications. However, the breeding and promotion of Chinese prickly ash have been severely restricted due to its confusing classification. Therefore, we assessed genetic diversity and phylogenetic relationship among 45 Chinese prickly ash samples collected from 6 main cultivated regions using 11 ISSR primers. These eleven selected primers generated a total of 102 scorable bands ranging from 150 to 2000 bp, corresponding to an average of approximately 9.3 bands per primer. The percentage of polymorphic loci for all samples ranged from 75% to 100%, with an average of 84.3%. The genetic similarity coefficient across all samples varied from 0.460 to 0.919. Remarkably, UPGMA analysis showed that 45 samples were divided into six clusters with a genetic similarity of 0.7. The closest genetic relationship was observed between Dahongpao collected from Qin’an and Tianshui, and the greatest genetic divergence was found between Dahongpao collected from Hengshui and Jiuyeqing collected from Jiangjin. It could serve as a basis for identifying Chinese prickly ash cultivars, breeding new cultivars and protecting the Chinese prickly ash resources in main regions.

scholarly journals Determination of Camellia oleifera Abel. Germplasm Resources of Genetic Diversity in China using ISSR Markers

2018 ◽  
Vol 46 (2) ◽  
pp. 501-508
Author(s):  
Junyong CHENG ◽  
Dezhi JIANG ◽  
Hua CHENG ◽  
Xihua ZHOU ◽  
Yingyou FANG ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Issr Markers ◽  
Germplasm Management ◽  
Camellia Oleifera ◽  
High Genetic Diversity ◽  
Upgma Cluster Analysis ◽  
Close Genetic Relationship ◽  
Issr Primers

Camellia oleifera is one of the four woody oil plants in the world, which is widely cultivated in South China. To examine the genetic diversity of C. oleifera in China, the diversity and genetic relationships among and within major populations of 109 varieties of C. oleifera were analyzed using ISSR markers. Twenty-three ISSR primers out of 49 primers yielded approximately 487 legible bands. A total of 335 of these bands were polymorphic markers, and the ratio of polymorphism was 68.86%. From the results, Zhejiang province showed the highest populations genetic diversity (H value 0.18), while Guangxi population showed the lowest genetic diversity (H 0.0851). Base on the bands, the genetic similarity coefficient ranged from 0.61 to 0.93 using NTSYS2.10e software. When coefficient was 0.75, 109 cultivars were divided into 11 categories and categories I contain 79 varieties by UPGMA cluster analysis. The test varieties divided into 7 sub-groups when categories were 0.75, which show a close genetic relationship. Results advised that Hunan is the main producing area of C. oleifera, with enriched C. oleifera variety and complex topography, and therefore has a high genetic diversity. Meanwhile, the main varieties of C. oleifera in Hubei are imported from Hunan, which results in fewer varieties and reduces the genetic diversity of C. oleifera. The ISSR profiles can improve C. oleifera germplasm management and provide potential determine correlations between different varieties and its distribution in different province.

scholarly journals Intersimple Sequence Repeat Markers for Fingerprinting and Determining Genetic Relationships of Walnut (Juglans regia) Cultivars

2002 ◽  
Vol 127 (1) ◽  
pp. 75-81 ◽  
Author(s):  
Daniel Potter ◽  
Fangyou Gao ◽  
Giovanna Aiello ◽  
Charles Leslie ◽  
Gale McGranahan
Keyword(s):  
Genetic Relationships ◽  
Juglans Regia ◽  
Issr Markers ◽  
Genetic Distances ◽  
Sequence Repeat ◽  
Geographic Origins ◽  
Pcr Products ◽  
Extensive Exchange ◽  
The One ◽  
Issr Primers

The utility of intersimple sequence repeat (ISSR) markers for identification of English or Persian walnut (Juglans regia L.) cultivars was explored. Four cultivars were screened with 47 ISSR primers; eight of these primers, which generated reproducible and informative data, were selected for further study. Two individuals from each of 48 cultivars, including many currently important in the California walnut industry as well as accessions from Europe and Asia, were then examined with the eight ISSR primers. Polymerase chain reaction (PCR) products were separated on agarose gels and stained with ethidium bromide. Fifty-four bands were scored as present or absent in each cultivar; of these, 31 (57%) were polymorphic among the 48 cultivars. Combined data from the eight ISSR primers provided a unique fingerprint for each of the cultivars tested. Fifteen of the cultivars could be distinguished from all others with just one primer, 31 with a minimum of two primers, and two required three primers. Pairwise genetic distances between the cultivars were calculated and a dendrogram was generated using the neighbor-joining algorithm. Some of the groupings in the dendrogram corresponded to groups which, based on known pedigrees, are genealogically closely related. Others included accessions from diverse genetic and/or geographic origins. These results can be attributed to a combination of the limitations of the ISSR method for inferring genetic relationships, on the one hand, and the complex history of walnut cultivar development involving extensive exchange and breeding of germplasm from different geographic regions, on the other.

scholarly journals Structure and Genetic Diversity of Nine Important Landraces of Capsicum Species Cultivated in the Yucatan Peninsula, Mexico

Agronomy ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 376 ◽  
Author(s):  
Lucero del C. López Castilla ◽  
René Garruña Hernández ◽  
Crescencio de la Cruz Castillo Aguilar ◽  
Aida Martínez-Hernández ◽  
Matilde Margarita Ortiz-García ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Yucatan Peninsula ◽  
Conservation Status ◽  
Sustainable Use ◽  
Issr Markers ◽  
Southern Mexico ◽  
Yucatán Peninsula ◽  
Capsicum Spp ◽  
Capsicum Species

Mexico has a wealth of Capsicum species, which has led to the development of a large number of chili pepper landraces. A great wealth of Capsicum germplasm can be found in southern Mexico in the Yucatan Peninsula, an important area of diversification of Capsicum annuum. Specifically, in the western Yucatan Peninsula, three of the five domesticated species of Capsicum (C. annuum, C. chinense and C. frutescens) have been reported. However, information on their genetic diversity, conservation status and potential use is lacking. To generate useful information toward the sustainable use, management and conservation of these species, we evaluated the structure, diversity and genetic relationships of nine accessions of Capsicum spp., of major importance cultivated in the western Yucatan Peninsula using 42 ISSR loci. The results indicated that these accessions consisted of three genetic groups that were defined by the respective species of each accession. The level of genetic diversity was moderate and distributed mainly among accessions. The ISSR markers detected a high level of polymorphism and allowed the genetic differentiation of the C. annuum complex. The results indicated that the accessions collected in the western Yucatan Peninsula constitute a valuable genetic resource that can be used in genetic improvement and conservation programs.

Assessment of the genetic diversity and genetic relationships of Lilium in China using ISSR markers

2014 ◽  
Vol 55 ◽  
pp. 184-189 ◽  
Author(s):  
Lihua Zhao ◽  
Huamin Liu ◽  
Guangze Cai ◽  
Mingzhong Xia
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Issr Markers

Genetic Diversity of an Alien Invasive Plant Mexican Sunflower (Tithonia diversifolia) in China

Weed Science ◽  
2012 ◽  
Vol 60 (4) ◽  
pp. 552-557 ◽  
Author(s):  
Jing Yang ◽  
Ling Tang ◽  
Ya-Li Guan ◽  
Wei-Bang Sun
Keyword(s):  
Genetic Diversity ◽  
Native Species ◽  
Invasive Plant ◽  
Genetic Relationships ◽  
Geographic Distance ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Chromosome Count ◽  
Group Method ◽  
Arithmetic Means

Mexican sunflower is a native species of North and Central America that was introduced into China early last century, but it has widely naturalized and become a harmful invasive plant in tropical and subtropical regions in South China. Inter-simple sequence repeat (ISSR) markers were employed to assess genetic diversity and variation in Mexican sunflower populations from China and neighboring regions. The karyotypes of populations were also studied. Our research showed high levels of genetic diversity in all populations. The lowest genetic diversity estimates were represented in two populations in Laos, suggesting prevention of new introductions into Laos is critical. Partitioning of genetic variance revealed that genetic variation was mostly found within populations, and unweighted pair group method with arithmetic means (UPGMA) analysis showed that the introductions into China and Laos were independent. There were no obvious correlations between genetic relationships and geographic distance of populations in China, consistent with the human associated dispersal history of Mexican sunflower. Previous cytological data and our chromosome count (2n = 34) and karyotype analysis showed chromosome stability among populations. The high levels of genetic diversity within invasive Mexican sunflower populations could be challenging for its management in China, and further expansion and potential negative effects on ecological systems of this plant should be monitored.

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