scholarly journals Protein degradability of grassland forage under simulated rotational spring grazing

2019 ◽  
Vol 64 (3) ◽  
pp. 255-263
Author(s):  
Bojan Stojanovic ◽  
Aleksandar Simic ◽  
Goran Grubic ◽  
Nenad Djordjevic ◽  
Aleksa Bozickovic ◽  
...  
Keyword(s):  
Streptomyces Griseus ◽  
Rotational Spring ◽  
Permanent Pasture ◽  
Cutting Experiment ◽  
True Protein ◽  
Ruminal Protein Degradability ◽  
Protease Assay ◽  
Degradable Protein ◽  
Protein Degradability

A cutting experiment was conducted to analyze the changes in the crude protein (CP) fraction content and in the estimated ruminal protein degradability of forage, obtained in conditions of simulated rotational spring grazing on permanent grassland. The field trial was conducted on permanent pasture during 2015 and included three cuttings as a simulated rotational spring grazing. For determination of protein degradability of pasture forage, the fractionation of the CP according to Cornell Net Carbohydrate and Protein System (CNCPS v6.5) and the Streptomyces griseus protease assay were used. Relative to CP, no significant differences were found among cuts for ammonia N content (A1 fraction) and for protein fraction C which is completely unavailable to the animals. Values for soluble true protein (A2 fraction) and cell wall-associated protein, which is acid detergent soluble (B2), were significantly increased (p<0.05) while a significant reduction (p<0.05) of the moderately degradable protein (B1) content was determined during the growing season. The lower rumen degradable protein (RDP) content of grassland herbage was obtained in the second cut which was significant (p<0.05) according to the CNCPS procedure. Obtained high solubility and degradability of CP in pasture require adequate content of readily available carbohydrates in rations for grazing ruminants to provide efficient utilization of consumed protein.

In vitro determination of ruminal protein degradability of alfalfa and prairie hay via a commercial protease in the presence or absence of cellulase or driselase.

1997 ◽  
Vol 75 (8) ◽  
pp. 2215 ◽  
Author(s):  
I E Abdelgadir ◽  
R C Cochran ◽  
E C Titgemeyer ◽  
E S Vanzant
Keyword(s):  
Ruminal Protein Degradability ◽  
Protein Degradability

Effect of Different Cereal:Forage Ratio and Protein Degradability on The Performance and Blood Metabolites of Iranian Sangsarri Lambs

1998 ◽  
Vol 1998 ◽  
pp. 85-85
Author(s):  
M. Danesh Mesgaran ◽  
G.H. Ieeragian
Keyword(s):  
Energy Supply ◽  
Basal Diet ◽  
Animal Production ◽  
Blood Metabolites ◽  
Metabolisable Energy ◽  
Ruminal Protein Degradability ◽  
Degradable Protein ◽  
Protein Degradability

The effects of carbohydrate-rich supplements on animal production are dependent on the type and proportion of supplement to basal diet (Obara et al., 1991). This supplementation should optimise the effective rumen degradable protein and fermentable metabolisable energy supply to the rumen and provide sufficient metabolisable protein to balance animal requirement (AFRC, 1992). The objective of this study was to investigate the effect of the feeding increased amount of ruminal available carbohydrate (RAC) in different ruminal protein degradability (PD) on Iranian Sangsarri lamb performance and blood metabolites.

PROTOZOAL CONTRIBUTION TO NITROGEN DIGESTION IN SHEEP

1984 ◽  
Vol 64 (5) ◽  
pp. 20-21 ◽  
Author(s):  
K. USHIDA ◽  
J. P. JOUANY ◽  
BERNADETTE LASSALAS ◽  
P. THIVEND
Keyword(s):  
Key Words ◽  
Dietary Protein ◽  
Microbial Nitrogen ◽  
Ruminal Protein Degradability ◽  
Degradable Protein ◽  
Protein Degradability

Compared with defaunated sheep, the large protozoal population of faunated sheep fed a diet high in poorly degradable protein decreased both microbial and dietary protein flows into the duodenum which indicated that protozoa increased ruminal protein degradability. Protozoal nitrogen represented 20% of the total microbial nitrogen in the duodenum. Key words: Protozoa, microbial proteins, sheep, protein degradability

scholarly journals Yield and nutritional value of permanent grassland forage under simulated rotational grazing

2018 ◽  
Vol 34 (1) ◽  
pp. 21-31
Author(s):  
Bojan Stojanovic ◽  
Aleksandar Simic ◽  
Goran Grubic ◽  
Aleksa Bozickovic ◽  
Ivan Krga
Keyword(s):  
Crude Protein ◽  
Nutritional Value ◽  
Forage Production ◽  
Rotational Spring ◽  
Permanent Grassland ◽  
The Third ◽  
Permanent Pasture ◽  
Optimal Period ◽  
Cutting Experiment ◽  
Nutrition Value

A cutting experiment was conducted to test the changes in botanical composition, yield and nutritional value of forage, obtained in conditions of simulated rotational spring grazing on permanent grassland. The experiment was carried out on permanent pasture in vicinity of Sabac, Serbia in 2015 included three cuttings as a simulated rotational spring grazing. The highest share of grasses was noted in the first cut and decreased in the second and third cut, with increased forbs participation, and relatively constant percentage of legumes. The highest dry matter (DM) yield was obtained for the first harvest, followed by the second cut, and the lowest forage production was determined for the third harvest, with only 11.04 and 17.42% of the first and second cut yield. There were not found the significant differences between cuts for herbage DM and crude protein content. Markedly lower value for non-protein N concentration (p<0.05) was determined in the third cut. The fiber content increased during the grazing season (p<0.05), with the highest value determined in the herbage obtained in the second cut. The highest energy values (p<0.05) had the herbage produced in the first cut (DM basis), wherein the lowest values were found in the forage from the second harvest. These results indicate that especially herbage yield of analyzed grassland as chemical composition and nutrition value are highly variable during the growing season. The accurately defined optimal period for using is necessary to provide the high-quality forage for grazing animals.

Influence of the concentration of the protease from Streptomyces griseus relative to ruminal protein degradability

1999 ◽  
Vol 77 (1-2) ◽  
pp. 99-113 ◽  
Author(s):  
G Licitra ◽  
P.J Van Soest ◽  
I Schadt ◽  
S Carpino ◽  
C.J Sniffen
Keyword(s):  
Streptomyces Griseus ◽  
Ruminal Protein Degradability ◽  
Protein Degradability

Effect of Different Cereal:Forage Ratio and Protein Degradability on The Performance and Blood Metabolites of Iranian Sangsarri Lambs

1998 ◽  
Vol 1998 ◽  
pp. 85-85
Author(s):  
M. Danesh Mesgaran ◽  
G.H. Ieeragian
Keyword(s):  
Energy Supply ◽  
Basal Diet ◽  
Animal Production ◽  
Blood Metabolites ◽  
Metabolisable Energy ◽  
Ruminal Protein Degradability ◽  
Degradable Protein ◽  
Protein Degradability

The effects of carbohydrate-rich supplements on animal production are dependent on the type and proportion of supplement to basal diet (Obara et al., 1991). This supplementation should optimise the effective rumen degradable protein and fermentable metabolisable energy supply to the rumen and provide sufficient metabolisable protein to balance animal requirement (AFRC, 1992). The objective of this study was to investigate the effect of the feeding increased amount of ruminal available carbohydrate (RAC) in different ruminal protein degradability (PD) on Iranian Sangsarri lamb performance and blood metabolites.

Determination of Protein Degradability of Some Subtropical Protein Sources byin situ Technique

2003 ◽  
Vol 23 (2) ◽  
pp. 209-215 ◽  
Author(s):  
M. Görgülü ◽  
L. Baykal ◽  
H. R. Kutlu ◽  
A. R. Tasdemir
Keyword(s):  
Protein Sources ◽  
Protein Degradability

The ability of the ficin enzyme technique to predict the in situ degradability of lucerne N

1999 ◽  
Vol 1999 ◽  
pp. 156-156
Author(s):  
G. Gizzi ◽  
E.R. Deaville ◽  
D.I. Givens
Keyword(s):  
Protein Degradation ◽  
Microbial Population ◽  
Reasonable Accuracy ◽  
Complex Process ◽  
Ruminal Protein Degradability ◽  
Protein Degradability

The assessment of protein degradability in the rumen is a complex process. The infinite combination of interaction between the rumen microbial population and the nature of the protein fed to the animal makes the estimation of ruminal protein degradability very arduous. At present the in situ technique is the most popular means of predicting ruminal nitrogen (N) degradation. However this procedure is slow, expensive and relies on the use of numerous surgically prepared animals. A number of studies (Assoumani et al., 1992; Aufrère and Cartailler, 1988) have shown that the use of in vitro methods using proteases can predict with reasonable accuracy the extent of protein degradation. The objective of this experiment was to examine the possibility of replacing the in situ technique with an in vitro procedure based on the use of the ficin protease to predict the extent of N degradation.

scholarly journals Application of FT near spectroscopy for determination of true protein and casein in milk

2008 ◽  
Vol 52 (No. 9) ◽  
pp. 284-291 ◽  
Author(s):  
K. Šustová ◽  
J. Růžičková ◽  
J. Kuchtík
Keyword(s):  
Cross Validation ◽  
Reference Data ◽  
Correlation Coefficients ◽  
Nir Spectroscopy ◽  
Rapid Analysis ◽  
Validation Method ◽  
Goat’S Milk ◽  
Goat's Milk ◽  
True Protein

Our study deals with a possibility of determining true protein and casein in cow’s, ewe’s and goat’s milk and in ewe’s colostrums by FT NIR spectroscopy. Samples of milk were analysed by FT NIR in the reflectance mode with the transflectance cuvette. The values of correlation coefficients of calibration were as follows: cow’s protein 0.943; cow’s casein 0.964; ewe’s protein 0.997; ewe’s casein 0.977; goat’s protein 0.989; goat’s casein 0.890; ewe’s colostrum protein 0.983. Calibration was tested using the same set of samples by the cross validation method. The values of correlation coefficients of validation were as follows: cow’s protein 0.923; cow’s casein 0.910; ewe’s protein 0.994; ewe’s casein 0.963; goat’s protein 0.972; goat’s casein 0.814; ewe’s colostrum protein 0.871. The NIRS results were compared with reference data and no significant differences between them were found (<i>P</i> = 0.05). Results of this study indicate that FT NIR spectroscopy can be used for a rapid analysis of protein and casein in cow’s, ewe’s and goat’s milk and ewe’s colostrum.

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