scholarly journals Genetic polymorphism of β-Lactoglobulin gene in indigenous Nigerian goat breeds

2019 ◽  
Vol 64 (4) ◽  
pp. 413-423
Author(s):  
Anthony Ezewud ◽  
Rukayyat Abubakar ◽  
Acheneje Egena ◽  
John Alabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Frequency ◽  
Fragment Length Polymorphism ◽  
West African ◽  
Restriction Digestion ◽  
Chain Reaction ◽  
The West ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Β Lactoglobulin

Polymorphism at the ?-Lactoglobulin (?-LG) gene of three Nigerian goat breeds, namely: the West African Dwarf, Sahel and Red Sokoto goats, was investigated using the Polymerase Chain Reaction-Random Fragment Length Polymorphism (PCR-RFLP) method. The restriction endonucleases used in the study were Rsal and Mspl, respectively. The results revealed the existence of only one polymorphic variant (allele A) with a gene frequency of 1.0 in all the three goat breeds studied. The amplified products were observed at 120 bp and the restriction digestion with Rsal revealed just one genotype at the ?-LG locus. It was concluded that there was the absence of polymorphism at the ?-LG locus of the goats investigated.

scholarly journals Identifikasi Keragaman Gen DGAT1 serta Asosiasinya terhadap Karakteristik Karkas dan Sifat Perlemakan Domba

2019 ◽  
Vol 6 (2) ◽  
pp. 259
Author(s):  
Asep Gunawan ◽  
Ratna Sholatia Harahap ◽  
Kasita Listyarini ◽  
Cece Sumantri
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphism ◽  
Fragment Length Polymorphism ◽  
Nucleotide Polymorphism ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Dgat1 Gene ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length

ABSTRAK Karakteristik karkas dan sifat perlemakan pada daging domba dikontrol oleh banyak gen salah satunya gen DGAT1 (Diacylglycerol Acyltransferasel 1). Penelitian ini bertujuan mengidentifikasi SNP (Single Nucleotide Polymorphism) gen DGAT1 pada titik mutasi g.8539 C>T dan asosiasinya terhadap karakteristik karkas dan sifat perlemakan pada domba Indonesia. Total sampel domba yang digunakan sebanyak 150 buah terdiri dari 35 sampel domba compass agrinak (DCA), 36 sampel domba barbados cross (DBC), 41 sampel domba komposit garut (DKG), 20 sampel domba ekor gemuk (DEG), dan 18 sampel domba ekor tipis (DET). Karakteristik karkas dan sifat perlemakan diukur dari domba jantan berumur 10-12 bulan. Identifikasi keragaman DGAT1|ALuI dianalisis dengan metode PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). Hasil keragaman gen DGAT1 bersifat polimorfik dalam DET dan DEG, sedangkan DCA, DBC, dan DKG bersifat monomorfik. Dua genotipe disebut CC dan  CT ditemukan dalam DET dan DEG. Titik mutasi gen DGAT1 berasosiasi (P<0.05) dengan karakteristik karkas, yaitu bobot dan panjang karkas. Selain itu, keragaman gen DGAT1 juga berasosiasi signifikan (P<0.05) dengan asam lemak jenuh, yaitu asam stearat (C18:0) dan asam arakidat (C20:0) dan asam lemak tak jenuh tunggal, yaitu asam oleat (C18:1n9c). Gen DGAT1 memiliki kontribusi dalam karakteristik karkas dan komposisi asam lemak pada domba.Kata Kunci: domba, gen DGAT1, karakteristik karkas, PCR-RFLP, sifat perlemakan                                                              ABSTRACT            Characteristic of carcass and fatness traits of sheep is regulated by many genes such as DGAT1 (Diacylglycerol Acyltransferasel 1) gene. The research was aimed to investigate SNP (Single Nucleotide Polymorphism) of DGAT1 and its association with characteristic of carcass and fatness traits in Indonesian sheep. A total sample of sheeps used 150 rams of 10–12 months consisted 35 samples of compas agrinak sheep (CAS), 36 of barbados cross (BCS), 41 of garut composite (GCS), 20  of javanese fat tailed (JFT), and 18 of javanese thin tailed (JTT). Identification variant of DGAT1|ALuI were performed by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The results of polymorphism of DGAT1 were found in JTT and JFT. However, SNP of DGAT1 in CAS, BCS and GCS were monomorfic. Two genotype namely CC and CT were found in JTT and JFT populations. A SNP of the DGAT1 was associated (P<0.05) with characteristic of carcass, including weight and length of carcass. The variant of DGAT1 was associated too with saturated fatty acids (SFA) including stearic acid (C18:0) and arachidic acid (C20:0), and mono unsaturated fatty acid (MUFA) including oleic acid (C18:1n9c). The DGAT1 gene was contribute to characteristic carcass and fatty acid composition in sheep.Keywords: DGAT1 gene, characteristic carcass, fatness traits, PCR-RFLP, sheep

scholarly journals A Study on ACE, ACTN3, and VDR Genes Polymorphism in Thai Weightlifters

2017 ◽  
Vol 15 (9) ◽  
pp. 609-626
Author(s):  
Luckhana PIMJAN ◽  
Chalermporn ONGVARRASOPONE ◽  
Wasun CHANTRATITA ◽  
Chumpol POLPRAMOOL ◽  
Pipat CHERDRUNGSI ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Elite Athlete ◽  
Fragment Length Polymorphism ◽  
Muscle Performance ◽  
Chain Reaction ◽  
Male And Female ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Genetic Combination ◽  
Genotype And Allele Frequencies

In order to excel in sport, apart from the correct training, the genetic combination of an individual may also be a factor in making an elite athlete. Weightlifting, as a popular sport, has a unique bio-mechanism dealing with muscle performance. The current study investigated the polymorphisms of the angiotensin-converting enzyme (ACE), the a-actinin-3 (ACTN3), and the vitamin D receptor (VDR) genes (individually or in combination) in Thai weightlifters. A total of 117 male and female national and junior Thai weightlifters, and 99 healthy sedentary people were recruited for this study. Genotyping was analyzed by Polymerase Chain Reaction (PCR) and Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). When compared to the junior and non-athletes group, the genotype and allele frequencies of ACE (DD), ACTN3 (RR), and VDR (ff) were more frequent in both male and female national weightlifters. In addition, the genotype combinations between ACE (DD) + VDR (ff), ACE (DD) + ACTN3 RR, and ACTN3 RR + VDR ff presented highly in both genders of national weightlifters. Taken together, our results suggest that the ACTN3 and VDR genotype, individually or in combination, may influence muscle performance in weightlifters, appearing to significantly contribute to better weightlifting performance.

scholarly journals Polymorphism of the kappa-casein gene in two Bosnian autochthonous cattle breeds

2010 ◽  
Vol 53 (3) ◽  
pp. 277-282
Author(s):  
M. Brka ◽  
A. Hodžić ◽  
N. Reinsch ◽  
E. P. Zečević ◽  
A. Dokso ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Small Population ◽  
Chain Reaction ◽  
Casein Gene ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Related Breed ◽  
Restriction Fragment Length

Abstract. Buša is an old endangered autochthonous breed of the western Balkan, especially Bosnia-Herzegovina, Kosovo and Albania. A related breed is Gatačko, derived from Buša × Tirolean Grey crossbreds. Fifteen purebred Buša cattle and thirteen Gatačko animals were genotyped for polymorphisms at the kappa-casein gene by a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) essay. The alleles A, B and C were found and the allelic frequencies were 0.46 (A), 0.46 (B) and 0.08 (C) in Buša cattle and 0.58 (A) and 0.42 (B) in Gatačko. Only AA, AB, BB and BC genotypes occurred. Further alleles were not detected and are therefore either absent in both populations or rare. The allele »B« found in this small population will be useful for a sire selection program in the future.

scholarly journals Análise de polimorfismo do gene da somatotropina em vacas Nelore e seu efeito sobre o peso à desmama de suas progênies

1999 ◽  
Vol 51 (6) ◽  
pp. 565-570
Author(s):  
F.J.C. Faria ◽  
S.E.F. Guimarães ◽  
R.M.G. Lima ◽  
G.B. Mourão ◽  
L.E.L. Pinheiro
Keyword(s):  
Polymerase Chain Reaction ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Pcr Rflp ◽  
Polymerase Chain ◽  
Restriction Fragment Length

Informações sobre peso à desmama de um rebanho Nelore foram utilizadas após ajuste para idade padrão de 205 dias, sexo da cria, idade da mãe, touro e mês de desmama, para separar as reprodutrizes em dois grupos, cujos filhos diferiam nesse peso. As médias ajustadas pelo método dos quadrados mínimos foram para os grupos pesado (P) e leve (L) de 163,21± 2,18kg e 134,44± 2,18kg, respectivamente, com 41 animais em cada grupo. Essas reprodutrizes foram submetidas à coleta de sangue para estudo de polimorfismos do gene da somatotropina bovina, pela técnica de PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). A amplificação de uma região entre o éxon III e V do gene da somatotropina permitiu analisar dois sítios de restrição. Para o sítio do éxon V, todos os animais foram identificados como monomórficos (Leu-Leu). Quanto ao sítio do íntron 3, foi possível identificar os seguintes genótipos 21 (+/-) e 60 (-/-), com as freqüências de 0,13 e 0,87 para os alelos (+) e (-), respectivamente. O peso dos filhos dos animais com o genótipo +/- foi de 152,42± 4,41kg e os -/- 147,60± 2,61kg. Os grupos P e L não diferiram entre si quanto às freqüências alélicas apresentadas. O genótipo das reprodutrizes não afetou o peso à desmama de suas crias, existindo portanto outros efeitos genéticos e não genéticos de maior magnitude.

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