scholarly journals Cd(II) extraction in PEG(1550)-(NH4)2SO4 aqueous two-phase systems using halide extractants

2008 ◽  
Vol 73 (3) ◽  
pp. 341-350 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Distribution Coefficients ◽  
Water Soluble ◽  
Phase System ◽  
Halide Ions ◽  
Two Phase ◽  
Water Soluble Polymer ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene

The extraction of Cd(II) was studied in an aqueous PEG-(NH4)2SO4 two-phase system, formed from a water-soluble polymer (poly(ethylene glycol), PEG) and an inorganic salt ((NH4)2SO4), in the presence of halide ions. In the absence of a suitable extracting agent, Cd(II) remains predominantly in the salt-rich phase of the extraction system. By addition of halide ions as extractants, Cd(II) is extracted into the PEG-rich phase due to the formation of cadmium halide species. The efficiency of the extractants increased in the order: Cl- < Br- < I-. From the distribution coefficients determined as a function of the concentration of the halide ions, the compositions of the extracted species were assumed and the "conditional" extractions constants calculated. The experimental results indicate that the extractability of Cd(II) in such extraction systems depends on the type of Cd(II) halide species (which is mainly determined by the acidity of salt stock solution) and of their stability.

scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

scholarly journals Protein Partitioning In A Droplet-Based Aqueous-Two Phase System Microfluidic Device

2021 ◽  
Author(s):  
David Hernández Cid ◽  
Roberto Carlos Gallo-Villanueva ◽  
José González-Valdez ◽  
Victor Hugo Pérez González ◽  
Marco Arnulfo Mata-Gómez
Keyword(s):  
Microfluidic Device ◽  
Rnase A ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Biological Entities ◽  
Phosphate Salts ◽  
Pegylated Form

Abstract Aqueous-Two Phase Systems (ATPS) is an important tool for the separation of biological entities as proteins, membranes, enzymes, among others. On the other hand, microfluidics is an emerging technology that studies and manipulates liquids either one single phase or dispersed fluids such as droplets at the micro or smaller scales. Applications of microfluidics in different areas such as molecular biology, biochemical analysis and bioprocess have increased in the last years. In this work, we proposed a droplet-based microfluidic approach to generate ATPS systems and to observe how two model proteins, native ribonuclease A (RNase A) and its PEGylated form (PEG-RNase A), behave and partition on these systems. Using polyethylenglycol (PEG) and potasium phosphate salts as the phase-forming chemicals, we were able to form ATPS systems inside the microfluidic device as commonly performed in conventional ATPS macrosystems. Even more, formation of ATPS systems in which one of the fluids was present as a droplet was also achieved. As expected, model proteins exhibited the same behavior as they do in a macrosystem, that is, they displaced to a particular phase according to their affinity for them. When native RNase A was placed in the salt-rich phase, it remained there, and migrated from the PEG-rich phase to the former. On its part, PEGylated RNase A remained in the PEGrich phase or migrated from salt-rich phase to the PEG-rich phase. These results open the possibility for a prospect of micro bioprocess to separate interest biomolecules.

scholarly journals Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiwen Bai ◽  
Yanhong Chao ◽  
Meiling Zhang ◽  
Changri Han ◽  
Wenshuai Zhu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Efficiency ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

scholarly journals Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning

1999 ◽  
Vol 30 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Maria Estela da Silva ◽  
Telma Teixeira Franco
Keyword(s):  
Partition Coefficient ◽  
Second Step ◽  
Step Method ◽  
Kluyveromyces Fragilis ◽  
Two Phase ◽  
Rich Phase ◽  
Extraction And Purification ◽  
Peg 4000 ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

This work investigated the partitioning of b-galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where b-galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of b-galactosidase (K = 2 x 10-5) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase.

The synthesis of water-soluble polymers of β-cyclodextrin and their use in aqueous two-phase systems

1989 ◽  
Vol 10 (3) ◽  
pp. 183-188 ◽  
Author(s):  
Björn Ekberg ◽  
Börje Sellergren ◽  
Lisabeth Olsson ◽  
Klaus Mosbach
Keyword(s):  
Water Soluble ◽  
Two Phase ◽  
Water Soluble Polymers ◽  
Soluble Polymers ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

Measurement and correlation of liquid-liquid equilibria for dextranpoly(ethylene glycol)water aqueous two-phase systems at 20°C

1995 ◽  
Vol 103 (1) ◽  
pp. 119-141 ◽  
Author(s):  
Takeshi Furuya ◽  
Yoshio Iwai ◽  
Yoshiaki Tanaka ◽  
Hirohisa Uchida ◽  
Sunao Yamada ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene
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