scholarly journals The extraction of Zn(II) in aqueous PEG (1550)-(NH4)2SO4 two-phase system using Cl- ions as extracting agent

2007 ◽  
Vol 72 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Aqueous Solutions ◽  
Extraction Efficiency ◽  
Phase Variation ◽  
Chloride Ions ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Experimental Parameters ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

The extraction of Zn(II) in an aqueous PEG (1550) - (NH4)2SO4 two-phase system as a function of several experimental parameters was studied. PEG-based aqueous two-phase systems are composed of two immiscible phases: a polymer-rich phase and a salt-rich phase, which can be used for extraction experiments. In the absence of a suitable extracting agent, for the system consisting of a mixture of equal volumes of 40 mass% PEG and 40 mass% (NH4)2SO4 aqueous solutions, Zn(II) remained predominantly in the salt-rich phase. Variation of the pH of the salt stock solution did not change very much the extraction efficiency. By adding chloride ions, an enhancement of the Zn(II) extraction was observed. The Zn(II) extraction efficiency in presence of Cl- depends on the acidity of the salt stock solution and on the concentration of chloride ions added into the system.

scholarly journals Partitioning Behavior of Papain in Ionic Liquids-Based Aqueous Two-Phase Systems

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Zhiwen Bai ◽  
Yanhong Chao ◽  
Meiling Zhang ◽  
Changri Han ◽  
Wenshuai Zhu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Extraction Efficiency ◽  
Phase System ◽  
Optimum Conditions ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Partitioning Behavior

This paper attempts to study and optimize the affinity partitioning conditions of papain in an aqueous two-phase system (ATPS). The effect of the amount of ionic liquids (ILs), the concentration of K2HPO4, temperature, pH, and the volume of papain solution were discussed concretely. The optimum conditions were determined as ionic liquid was 1.4 g and K2HPO4was 1.4 g, the extraction efficiency of papain could reach 98.33% with pH unadjusted. The temperature and the pH of the solution are major parameters that influence the partitioning of protein in ILs-based ATPSs. The partition of papain to the IL-rich phase was enhanced by increasing the amount of ILs, the concentration of K2HPO4, and temperature, especially at its isoelectric point.

Extraction and stability of selected proteins in ionic liquid based aqueous two phase systems

2014 ◽  
Vol 16 (5) ◽  
pp. 2670-2679 ◽  
Author(s):  
Rupali K. Desai ◽  
Mathieu Streefland ◽  
Rene H. Wijffels ◽  
Michel H. M. Eppink
Keyword(s):  
Ionic Liquid ◽  
Aqueous Solutions ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
The Stability

This article discusses the extraction of Rubisco in an ionic liquid (IL) based aqueous two phase system and the stability of proteins in aqueous solutions of IL.

scholarly journals Protein Partitioning In A Droplet-Based Aqueous-Two Phase System Microfluidic Device

2021 ◽  
Author(s):  
David Hernández Cid ◽  
Roberto Carlos Gallo-Villanueva ◽  
José González-Valdez ◽  
Victor Hugo Pérez González ◽  
Marco Arnulfo Mata-Gómez
Keyword(s):  
Microfluidic Device ◽  
Rnase A ◽  
Phase System ◽  
Two Phase ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Biological Entities ◽  
Phosphate Salts ◽  
Pegylated Form

Abstract Aqueous-Two Phase Systems (ATPS) is an important tool for the separation of biological entities as proteins, membranes, enzymes, among others. On the other hand, microfluidics is an emerging technology that studies and manipulates liquids either one single phase or dispersed fluids such as droplets at the micro or smaller scales. Applications of microfluidics in different areas such as molecular biology, biochemical analysis and bioprocess have increased in the last years. In this work, we proposed a droplet-based microfluidic approach to generate ATPS systems and to observe how two model proteins, native ribonuclease A (RNase A) and its PEGylated form (PEG-RNase A), behave and partition on these systems. Using polyethylenglycol (PEG) and potasium phosphate salts as the phase-forming chemicals, we were able to form ATPS systems inside the microfluidic device as commonly performed in conventional ATPS macrosystems. Even more, formation of ATPS systems in which one of the fluids was present as a droplet was also achieved. As expected, model proteins exhibited the same behavior as they do in a macrosystem, that is, they displaced to a particular phase according to their affinity for them. When native RNase A was placed in the salt-rich phase, it remained there, and migrated from the PEG-rich phase to the former. On its part, PEGylated RNase A remained in the PEGrich phase or migrated from salt-rich phase to the PEG-rich phase. These results open the possibility for a prospect of micro bioprocess to separate interest biomolecules.

scholarly journals Cd(II) extraction in PEG(1550)-(NH4)2SO4 aqueous two-phase systems using halide extractants

2008 ◽  
Vol 73 (3) ◽  
pp. 341-350 ◽  
Author(s):  
Laura Bulgariu ◽  
Dumitru Bulgariu
Keyword(s):  
Distribution Coefficients ◽  
Water Soluble ◽  
Phase System ◽  
Halide Ions ◽  
Two Phase ◽  
Water Soluble Polymer ◽  
Rich Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Poly Ethylene

The extraction of Cd(II) was studied in an aqueous PEG-(NH4)2SO4 two-phase system, formed from a water-soluble polymer (poly(ethylene glycol), PEG) and an inorganic salt ((NH4)2SO4), in the presence of halide ions. In the absence of a suitable extracting agent, Cd(II) remains predominantly in the salt-rich phase of the extraction system. By addition of halide ions as extractants, Cd(II) is extracted into the PEG-rich phase due to the formation of cadmium halide species. The efficiency of the extractants increased in the order: Cl- < Br- < I-. From the distribution coefficients determined as a function of the concentration of the halide ions, the compositions of the extracted species were assumed and the "conditional" extractions constants calculated. The experimental results indicate that the extractability of Cd(II) in such extraction systems depends on the type of Cd(II) halide species (which is mainly determined by the acidity of salt stock solution) and of their stability.

scholarly journals Purification of microbial b-galactosidase from Kluyveromyces fragilis by bioaffinity partitioning

1999 ◽  
Vol 30 (4) ◽  
pp. 324-331 ◽  
Author(s):  
Maria Estela da Silva ◽  
Telma Teixeira Franco
Keyword(s):  
Partition Coefficient ◽  
Second Step ◽  
Step Method ◽  
Kluyveromyces Fragilis ◽  
Two Phase ◽  
Rich Phase ◽  
Extraction And Purification ◽  
Peg 4000 ◽  
Aqueous Two Phase Systems ◽  
Phase Systems

This work investigated the partitioning of b-galactosidase from Kluyveromyces fragilis in aqueous two-phase systems (ATPS) by bioaffinity. PEG 4000 was chemically activated with thresyl chloride, and the biospecific ligand p-aminophenyl 1-thio-b-D-galactopyranoside (APGP) was attached to the activated PEG 4000. A new two-step method for extraction and purification of the enzyme b-galactosidase from Kluyveromyces fragilis was developed. In the first step, a system composed of 6% PEG 4000-APGP and 8% dextran 505 was used, where b-galactosidase was strongly partitioned to the top phase (K = 2,330). In the second step, a system formed of 13% PEG-APGP and 9% phosphate salt was used to revert the value of the partition coefficient of b-galactosidase (K = 2 x 10-5) in order to provide the purification and recovery of 39% of the enzyme in the bottom salt-rich phase.

Microfluidic generation of aqueous two-phase system (ATPS) droplets by controlled pulsating inlet pressures

Lab on a Chip ◽  
2015 ◽  
Vol 15 (11) ◽  
pp. 2437-2444 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Steven G. Jones ◽  
Dae Kun Hwang ◽  
Scott S. H. Tsai
Keyword(s):  
Interfacial Tension ◽  
Phase System ◽  
Flow Focusing ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Microfluidic Flow ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ultralow Interfacial Tension

Simple microfluidic flow focusing generation of droplets from ultralow interfacial tension aqueous two phase systems (ATPS).

Purification and Concentration of C-Phycocyanin from Spirulina platensis Using Aqueous Two-Phase System

2011 ◽  
Vol 138-139 ◽  
pp. 995-1001 ◽  
Author(s):  
Wei Jie Wang ◽  
Xiao Le Zhang ◽  
Chang Bo Xu ◽  
Hong Yan Cheng
Keyword(s):  
High Purity ◽  
Separation Process ◽  
Phase System ◽  
Two Phase ◽  
Sodium Tartrate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Optimized Conditions ◽  
Potassium Sodium Tartrate ◽  
Partitioning Behavior

In this article, the partitioning behavior of C-phycocyanin (C-PC) in aqueous two-phase systems (ATPS) with different component was investigated to evaluate the applicability of ATPS for extracting high purity of C-PC without chromatography. Under the optimized conditions (pH = 6; PEG-2000, 16 %, w/w; potassium sodium tartrate 21 %, w/w), a maximum purity of 5.01 was achieved after the whole separation process and the yield of the C-PC was 76.94 %.

Gas Column/Mass Spectrometry Analysis of Bio Oil Treated by Aqueous Two Phase System

2011 ◽  
Vol 236-238 ◽  
pp. 54-58
Author(s):  
Shao Peng Liu ◽  
Zhi Min Zong ◽  
Shan Shan Zhang ◽  
Yue Xu ◽  
Gui Zhen Gong ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Analysis ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Spectrometry Analysis ◽  
Bio Oil ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Ethanol System

Bio oil aqueous two phase systems were formed with ethanol and the salts of (NH4)2SO4, K2HPO4 and NaH2PO4 in bio oil on the ratio of 10:5:10(by mass). After phase separation, both the upper and the lower layers were analyzed by gas column/mass spectrometry (GC/MS). As a result, both K2HPO4/ethanol and NaH2PO4/ethanol system can enrich phenols and levoglucosan in the upperand lower layers,respectly, while NaH2PO4/ethanol system seldom changes the components of bio oil; the (NH4)2SO4/ethanol system is quit different with the other two and we have detected some components with nitrogen. Hence, NaH2PO4/ethanol is an advantage aqueous two phase system for bio oil treatment.

scholarly journals Extraction of Fe (III) and Zn (II) by ionic liquids in a system based on polypropylene glycol 425 and sodium chloride.

2020 ◽  
Vol 11 (3-2020) ◽  
pp. 72-75
Author(s):  
I. V. Zinov’eva ◽  
◽  
M. I. Fedorova ◽  
Yu. A. Zakhodyaeva ◽  
A.A. Voshkin ◽  
...  
Keyword(s):  
Experimental Study ◽  
Ionic Liquids ◽  
Sodium Chloride ◽  
Phase System ◽  
Polypropylene Glycol ◽  
Two Phase ◽  
Study Results ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Extraction Of Metals

This work presents the experimental study results of Fe(III) and Zn(II) extraction by ionic liquids based on trioctylmethylammonium chloride and rhodanide in an aqueous two-phase system based on polypropylene glycol 425 and sodium chloride. Based on the results obtained, it was concluded that these extractants are effective for the extraction of metals from aqueous solutions, and aqueous two-phase systems represent a “green” alternative to traditional extraction systems.

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