Specific Antibody Responses and Generation of Antigenic Variants in Chickens Immunized against a Virulent Avian Influenza Virus

1990 ◽  
Vol 34 (1) ◽  
pp. 80 ◽  
Author(s):  
Virginia S. Hinshaw ◽  
Martha G. Sheerar ◽  
Diane Larsen
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Specific Antibody ◽  
Antibody Responses

scholarly journals Specific antibody response of mice after immunization with COS-7 cell derived avian influenza virus (H5N1) recombinant proteins

2007 ◽  
Vol 5 (1) ◽  
pp. 10 ◽  
Author(s):  
Navin Horthongkham ◽  
Tananun Srihtrakul ◽  
Niracha Athipanyasilp ◽  
Sontana Siritantikorn ◽  
Wannee Kantakamalakul ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Antibody Response ◽  
Avian Influenza Virus ◽  
Recombinant Proteins ◽  
Specific Antibody ◽  
Specific Antibody Response ◽  
Virus H5n1 ◽  
Avian Influenza Virus H5n1

scholarly journals Avian Influenza H6 Viruses Productively Infect and Cause Illness in Mice and Ferrets

2008 ◽  
Vol 82 (21) ◽  
pp. 10854-10863 ◽  
Author(s):  
Laura Gillim-Ross ◽  
Celia Santos ◽  
Zhongying Chen ◽  
Amy Aspelund ◽  
Chin-Fen Yang ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Protective Immunity ◽  
Influenza Pandemic ◽  
Cross Reactivity ◽  
Influenza Viruses ◽  
Antibody Responses ◽  
High Rate ◽  
Pandemic Preparedness

ABSTRACT Influenza pandemic preparedness has focused on influenza virus H5 and H7 subtypes. However, it is not possible to predict with certainty which subtype of avian influenza virus will cause the next pandemic, and it is prudent to include other avian influenza virus subtypes in pandemic preparedness efforts. An H6 influenza virus was identified as a potential progenitor of the H5N1 viruses that emerged in Hong Kong in 1997. This virus continues to circulate in the bird population in Asia, and other H6 viruses are prevalent in birds in North America and Asia. The high rate of reassortment observed in influenza viruses and the prevalence of H6 viruses in birds suggest that this subtype may pose a pandemic risk. Very little is known about the replicative capacity, immunogenicity, and correlates of protective immunity for low-pathogenicity H6 influenza viruses in mammals. We evaluated the antigenic and genetic relatedness of 14 H6 influenza viruses and their abilities to replicate and induce a cross-reactive immune response in two animal models: mice and ferrets. The different H6 viruses replicated to different levels in the respiratory tracts of mice and ferrets, causing varied degrees of morbidity and mortality in these two models. H6 virus infection induced similar patterns of neutralizing antibody responses in mice and ferrets; however, species-specific differences in the cross-reactivity of the antibody responses were observed. Overall, cross-reactivity of neutralizing antibodies in H6 virus-infected mice did not correlate well with protection against heterologous wild-type H6 viruses. However, we have identified an H6 virus that induces protective immunity against viruses in the North American and Eurasian lineages.

scholarly journals Immunogenicity of Fowlpox Virus Expressing the Avian Influenza Virus H5 Gene (TROVAC AIV-H5) in Cats

2005 ◽  
Vol 12 (11) ◽  
pp. 1340-1342 ◽  
Author(s):  
Kemal Karaca ◽  
David E. Swayne ◽  
Deborah Grosenbaugh ◽  
Michel Bublot ◽  
Amy Robles ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Hemagglutination Inhibition ◽  
Virus Antigen ◽  
Antibody Responses ◽  
Morbidity And Mortality ◽  
Fowlpox Virus ◽  
Hemagglutinin Gene

ABSTRACT Vaccination of cats with fowlpox virus expressing the avian influenza (AI) virus H5 hemagglutinin gene (TROVAC AI) resulted in detectable hemagglutination inhibition (HI) antibody responses to the homologous A/Turkey/Ireland/1378/83 (H5N8) (A/tky/Ire/83) AI virus antigen. The HI antibody responses to heterologous A/Chicken/Indonesia/7/03 (H5N1) (A/ck/Indonesia/03) AI virus antigen were also detected in all vaccinated cats, but only after booster vaccinations. The vaccine described in this study and other poxvirus-vectored vaccines may be of value for the prophylaxis of AI virus-associated morbidity and mortality in mammals.

scholarly journals Detection of Specific type-A Antigen and Specific Antibody against Avian Influenza virus in Native Duck at Netrokona district of Bangladesh

2016 ◽  
Vol 4 (2) ◽  
pp. 5-10
Author(s):  
MM Mafizul Islam ◽  
Mir Rowshan Akter ◽  
Md Mostafizer Rahman ◽  
Md Atiqul Haque ◽  
Md Karim Uddin ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Positive Result ◽  
Avian Influenza Virus ◽  
Antibody Titer ◽  
Specific Antibody ◽  
Type A ◽  
Aged Group ◽  
Influenza Type ◽  
Test Kit

The present study was conducted on unvaccinated native ducks of different age groups to determine specific antibody titer level against Avian Influenza virus (AIV) by indirect Enzyme Linked Immunosorbent Assay (iELISA) and to detect avian influenza type A virus antigen by rapid AIV antigen test kit at Netrokona district of Bangladesh. This study showed that AIV specific antibody positive cases were 78 out of 90 blood serum samples and the highest antibody titer was 2323 and lowest antibody titer was 256. The total 86.67% sera samples were showed positive result. The study showed that 66.66% sera sample were positive against AIV at 3-4 month of aged group and the highest, lowest and mean antibody titer were 1428, 256 and 906.3 respectively. On the other hand 78% sera sample were positive against AIV at 5-6 month aged group and the highest, lowest and mean antibody titer were 1675 , 451 and 1083.6 respectively. The sera sample collected from 7-8 month aged group showed 88.9% positive and the highest, lowest and mean antibody titer were 1857, 578 and 1285.5 respectively. The sera sample collected from 9-10 month of aged group showed 100% positive against AIV and the highest, lowest and mean antibody titer were 197l, 638 and 1571.5 respectively .The sera sample collected from duck of ?11 month aged group were 100% positive against AIV and the highest, lowest and mean antibody titer were 2323, 1423 and 1813.7 respectively. Tracheal and cloacal swabs from ducks with antibody titer more than 1813.778 were tested for the avian influenza type A antigen by Anigen Rapid AIV Ag test kit. The above sample showed 20% positive result. In conclusion it is evident that Avian influenza virus-specific antibody was successfully detected through commercially available Avian influenza virus antibody test kit (ELISA Kit) and the virus induced a significant antibody titer indicating the affecting virus was absolutely AIV.

scholarly journals Evaluation of a Subunit H5 Vaccine and an Inactivated H5N2 Avian Influenza Marker Vaccine in Ducks Challenged with Vietnamese H5N1 Highly Pathogenic Avian Influenza Virus

2010 ◽  
Vol 2010 ◽  
pp. 1-10 ◽  
Author(s):  
Tze-Hoong Chua ◽  
Connie Y. H. Leung ◽  
H. E. Fang ◽  
Chun-Kin Chow ◽  
Siu-Kit Ma ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Influenza Vaccine ◽  
Avian Influenza Virus ◽  
Recombinant Baculovirus ◽  
Antibody Responses ◽  
Small Scale ◽  
Virus Shedding ◽  
Marker Vaccine ◽  
A Subunit

The protective efficacy of a subunit avian influenza virus H5 vaccine based on recombinant baculovirus expressed H5 haemagglutinin antigen and an inactivated H5N2 avian influenza vaccine combined with a marker antigen (tetanus toxoid) was compared with commercially available inactivated H5N2 avian influenza vaccine in young ducks. Antibody responses, morbidity, mortality, and virus shedding were evaluated after challenge with a Vietnamese clade 1 H5N1 HPAI virus [A/VN/1203/04 (H5N1)] that was known to cause a high mortality rate in ducks. All three vaccines, administered with water-in-oil adjuvant, provided significant protection and dramatically reduced the duration and titer of virus shedding in the vaccinated challenged ducks compared with unvaccinated controls. The H5 subunit vaccine was shown to provide equivalent protection to the other two vaccines despite the H5 antibody responses in subunit vaccinated ducks being significantly lower prior to challenge. Ducks vaccinated with the H5N2 marker vaccine consistently produced antitetanus toxoid antibody. The two novel vaccines have attributes that would enhance H5N1 avian influenza surveillance and control by vaccination in small scale and village poultry systems.

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