Accumulation of Calcium in the Antennal Gland during the Molting Cycle of the Freshwater Crayfish Procambarus clarkii

1997 ◽  
Vol 116 (3) ◽  
pp. 248 ◽  
Author(s):  
James V. Rogers ◽  
Michele G. Wheatly
Keyword(s):  
Procambarus Clarkii ◽  
Freshwater Crayfish ◽  
Molting Cycle ◽  
Antennal Gland

Isolation, visualization, characterization, and osmotic reactivity of crayfish BLMV

1998 ◽  
Vol 274 (3) ◽  
pp. R725-R734
Author(s):  
Michele G. Wheatly ◽  
Jennifer R. Weil ◽  
Phyllis B. Douglas
Keyword(s):  
Procambarus Clarkii ◽  
Basolateral Membrane ◽  
Membrane Vesicles ◽  
Freshwater Crayfish ◽  
Basolateral Membrane Vesicles ◽  
Marine Crustaceans ◽  
Antennal Gland ◽  
Discontinuous Sucrose Gradient ◽  
Uptake Studies ◽  
Inside Out

Procedures were developed to isolate basolateral membrane vesicles (BLMV) from gill, hepatopancreas, and antennal gland of intermolt freshwater crayfish, Procambarus clarkii. Individual procedures involved a discontinuous sucrose gradient (gill), a 65% sucrose cushion (hepatopancreas), or differential centrifugation (antennal gland). BLMV were visualized, characterized (37°C), and tested for osmotic reactivity with a view to using them for Ca2+ uptake studies. Mean diameters of BLMV were 159 nm (gill), 363 nm (hepatopancreas), and 226 nm (antennal gland). Enrichments of basolateral membranes and mitochondria in BLMV were, respectively, 18- and 1.7-fold for gill, 9- and 0.4-fold for hepatopancreas, and 10- and 1-fold for antennal gland. Apical contamination was negligible in BLMV. Percentages of resealing of vesicles as inside out, right side out, or leaky/sheets were 17:27:56% (gill), 14:26:60% (hepatopancreas), and 21:39:40% (antennal gland). Vesicles exhibited osmotic reactivity, as indicated by a linear relationship between vesicular45Ca2+uptake and osmolality. Nonspecific45Ca2+binding was 20% in gill, 39% in hepatopancreas, and 31% in antennal gland. Data were compared with published values for marine crustaceans.

Iinfectious hypodermal and haematopoietic necrosis virus (IHHNV) infection in freshwater crayfish Procambarus clarkii

Aquaculture ◽  
2017 ◽  
Vol 477 ◽  
pp. 76-79 ◽  
Author(s):  
Bo-Kun Chen ◽  
Zhen Dong ◽  
Da-Peng Liu ◽  
Yong-Bin Yan ◽  
Neng-Yuan Pang ◽  
...  
Keyword(s):  
Procambarus Clarkii ◽  
Freshwater Crayfish ◽  
Necrosis Virus

Characterization of Oscillatory Olfactory Interneurones in the Protocerebrum of the Crayfish

1992 ◽  
Vol 167 (1) ◽  
pp. 15-38
Author(s):  
DEFOREST MELLON ◽  
DAVID C. SANDEMAN ◽  
RENATE E. SANDEMAN
Keyword(s):  
Procambarus Clarkii ◽  
Lucifer Yellow ◽  
Freshwater Crayfish ◽  
University Of Virginia ◽  
Olfactory Pathway ◽  
Electrophysiological Recordings ◽  
Burst Period ◽  
Electron Microscopical ◽  
The Brain

1. We obtained intracellular electrophysiological recordings from local interneurones within the hemi-ellipsoid neuropile of the brain in the freshwater crayfish Cherax destructor and Procambarus clarkii. The recordings were made from perfused, isolated head preparations that provided several indications of a healthy physiological condition. 2. The hemi-ellipsoid interneurones are spontaneously active, generating bursts of action potentials at regular intervals. The inter-burst period differs among neurones, varying from about 1.0 s at the shortest periods to around 30 s for the longest periods. 3. Evidence from both electrophysiological recordings and from injection of Lucifer Yellow and Neurobiotin dyes into hemi-ellipsoid interneurones suggests that some of the cells in the populations are electrically coupled to one another. 4. Hemi-ellipsoid interneurones are driven postsynaptically by axons within the lateral protocerebral tract. Experiments with focal electrical stimulation strongly suggest that the pathways responsible include axons of the olfactory-globular tract. These findings support our previous electron microscopical data showing that olfactory-globular tract axons are presynaptic to the hemi-ellipsoid interneurones. 5. These findings support the conclusion that hemi-ellipsoid interneurones are an integral link in the central olfactory pathway of the crayfish. Note: Present address and address for reprint requests: Department of Biology, Gilmer Hall, University of Virginia, Charlottesville, VA 22901, USA.

Cadmium-induced oxidative stress, histopathology, and transcriptome changes in the hepatopancreas of freshwater crayfish (Procambarus clarkii)

2019 ◽  
Vol 666 ◽  
pp. 944-955 ◽  
Author(s):  
Yu Zhang ◽  
Zheyu Li ◽  
Sergey Kholodkevich ◽  
Andrey Sharov ◽  
Yujie Feng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Procambarus Clarkii ◽  
Freshwater Crayfish

Mercury effects on glutathione in the freshwater crayfish(Procambarus clarkii). In vivoandin vitrostudy

1990 ◽  
Vol 29 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Francisco J. Romero ◽  
Elena Monsalve ◽  
Carlos Hermenegildo ◽  
Francisco J. Puertas ◽  
Mar M. Almar ◽  
...  
Keyword(s):  
Procambarus Clarkii ◽  
Freshwater Crayfish
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