Acute Radiation Effects on the Content and Release of Plasminogen Activator Activity in Cultured Aortic Endothelial Cells

1985 ◽  
Vol 101 (2) ◽  
pp. 394 ◽  
Author(s):  
Chung-hsin Ts'ao ◽  
William F. Ward
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Radiation Effects ◽  
Acute Radiation ◽  
Aortic Endothelial Cells ◽  
Plasminogen Activator Activity ◽  
Aortic Endothelial

The Dexamethasone-Induced Inhibitor of Plasminogen Activator in Hepatoma Cells Is Antigenically-Related to an Inhibitor Produced by Bovine Aortic Endothelial Cells

1986 ◽  
Vol 55 (01) ◽  
pp. 008-011 ◽  
Author(s):  
David J Loskutoff ◽  
Karen Roegner ◽  
Larry A Erickson ◽  
Raymond R Schleef ◽  
Anna Huttenlocher ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasminogen Activator ◽  
Specific Inhibitor ◽  
Hepatoma Cells ◽  
Biochemical Properties ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Immunological Relationship ◽  
Relationship Of ◽  
Aortic Endothelial

SummaryGlucocorticoids decrease plasminogen activator (PA) activity in HTC rat hepatoma cells by inducing a specific inhibitor of PA activity (PAI). This inhibitor is similar in several biochemical properties to the PAI purified from bovine aortic endothelial cells (BAEs). We have used reverse fibrin autography and antiserum against BAE PAI to establish more fully the biochemical and immunological relationship of these inhibitors. Both inhibitors migrated with an apparent Mr of approximately 50,000, and the activity of both PAIs was stimulated by treatment with SDS suggesting that each of these molecules exists in both an active and a latent form. Antiserum to the BAE PAI immunoprecipi-tated all of the HTC PAI demonstrable by reverse fibrin autography. Finally, using this antiserum in a functional immunoassay, we have demonstrated that dexamethasone increases both active and latent PAI made by HTC cells. These results, indicate that HTC PAI and BAE PAI are antigenically as well as biochemically related molecules.

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