Evaluations of Cellular Proliferation and Chromosome Breakages after in Vitro Exposure of Human Lymphocytes to Calcium or Zinc DTPA

1984 ◽  
Vol 99 (1) ◽  
pp. 129 ◽  
Author(s):  
L. Gayle Littlefield ◽  
E. E. Joiner ◽  
Shirley P. Colyer ◽  
R. J. DuFrain ◽  
L. C. Washburn
Keyword(s):  
Cellular Proliferation ◽  
Human Lymphocytes ◽  
In Vitro Exposure ◽  
Chromosome Breakages

Occupational and in vitro exposure to styrene assessed by unscheduled DNA synthesis in resting human lymphocytes

1982 ◽  
Vol 3 (6) ◽  
pp. 681-685 ◽  
Author(s):  
Ronald W. Pero ◽  
Tomas Bryngelsson ◽  
Benkt Högstedt ◽  
Bengt Åkesson
Keyword(s):  
Dna Synthesis ◽  
Human Lymphocytes ◽  
Unscheduled Dna Synthesis ◽  
In Vitro Exposure

scholarly journals Micronucleus Assay as a Biological Indicator for In Vitro Exposure of Human Lymphocytes to Gamma Rays

2012 ◽  
Vol 6 (1) ◽  
pp. 51-55
Author(s):  
Abbas N. Balasem
Keyword(s):  
Gamma Rays ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Micronucleus Assay ◽  
Biological Indicator ◽  
Peripheral Blood Lymphocytes ◽  
Radiation Accidents ◽  
In Vitro Exposure ◽  
Different Doses

Micronucleus Assay was employed to detect the effects of acute exposure of human peripheral blood lymphocytes in vitro to Cs -137 gamma rays. Human whole blood samples were irradiated with different doses of gamma rays namely ) 0.1, 0.2, 0.3, 0.4, 0.5, 1.00) Gy, respectively in addition to a control non-irradiated sample. The samples were tissue cultured and cytokinesis blocked method was used to investigate the frequency of micronuclei. In vitro exposure of lymphocytes to this doses led to elevation of micronuclei in comparison with non –irradiated samples However, inclusion of mono-, tri-,and quadrinucleated cells in micronucleus assay probably gives more satisfying result than restriction the test on binucleated cells. Computed programmed were employed to establish dose – response relationships to be used as biological dosimeter during radiation accidents.

High susceptibility of chromosome 16 to radiation-induced chromosome rearrangements in human lymphocytes under in vivo and in vitro exposure

2004 ◽  
Vol 108 (4) ◽  
pp. 287-292 ◽  
Author(s):  
M.L. Camparoto ◽  
S.A. Takahashi-Hyodo ◽  
J.G. Dauwerse ◽  
A.T. Natarajan ◽  
E.T. Sakamoto-Hojo
Keyword(s):  
Human Lymphocytes ◽  
Chromosome Rearrangements ◽  
Chromosome 16 ◽  
High Susceptibility ◽  
In Vitro Exposure ◽  
Radiation Induced

Correlation Between Cellular Functions and Morphological Changes of Human Trophoblast in Vitro

1975 ◽  
Vol 33 ◽  
pp. 600-601
Author(s):  
John C. Garancis ◽  
Robert O. Hussa ◽  
Michael T. Story ◽  
Donald Yorde ◽  
Roland A. Pattillo
Keyword(s):  
Electron Microscopy ◽  
Cellular Proliferation ◽  
Morphological Changes ◽  
Culture Fluid ◽  
Cellular Functions ◽  
Human Trophoblast ◽  
Transmission Electron ◽  
Marked Activity ◽  
Malignant Trophoblast

Human malignant trophoblast cells in continuous culture were incubated for 3 days in medium containing 1 mM N6-O2'-dibutyryl cyclic adenosine 3':5'-monophosphate (dibutyryl cyclic AMP) and 1 mM theophylline. The culture fluid was replenished daily. Stimulated cultures secreted many times more chorionic gonadotropin and estrogens than did control cultures in the absence of increased cellular proliferation. Scanning electron microscopy revealed remarkable surface changes of stimulated cells. Control cells (not stimulated) were smooth or provided with varying numbers of microvilli (Fig. 1). The latter, usually, were short and thin. The surface features of stimulated cells were considerably different. There was marked increase of microvilli which appeared elongated and thick. Many cells were covered with confluent polypoid projections (Fig. 2). Transmission electron microscopy demonstrated marked activity of cytoplasmic organelles. Mitochondria were increased in number and size; some giant forms with numerous cristae were observed.

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